Usages： For determination of coliform confirmatory test for multi-tube fermentation.
Principle: Peptone provide carbon and nitrogen sources; lactose as fermentable sugars; ox bile powder and brilliant green inhibit the non-Enterobacteriaceae.
Formulation (per liter): Peptone: 10g Lactose: 10g Ox bile powder: 20g Brilliant green: 0.0133g Final pH7.2 ± 0.2
How to use: 1.Suspend 40g of the product, add 1 L of distilled water, stirring heated to boiling until completely dissolved, packed in a test tube with a small inverted tube, autoclave at 121 ℃ for 15min, cool to room temperature and set aside. 2.Sample handling and dilution. Slightly. 3.Transplanted LST broth tube to brilliant green lactose bile broth tube by using a inoculating loop. 4.the tube was placed in an incubator culture at 36 ± 1 ℃ for 24h. 5.Observe the results. Press production trachea number, check table report MPN MPN value per gram (mL) sample of coliform bacteria.
Quality control: Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows: Bacterial Name Bacterial No. Growth Status Gassing Escherichia coli ATCC25922 good +
Salmonella typhimurium CMCC (B) 50115 good —
Staphylococcus aureus ATCC6538 inhibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Specifications: 250g/bottle
Other Products
TruScript Reverse Transcriptase and Kits
Product Info
Document
Product Info
Overview
Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
Time Savings – Set up RT reactions in a shorter time since less pipetting steps are required
Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility
TruScript Reverse Transcriptase is Available in Three Convenient Formats:
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
Which TruScript Kit is Best for Your Needs?
TruScript Reverse Transcriptase
TruScript First Strand cDNA Synthesis Kit for mRNA
TruScript First Strand cDNA Synthesis Kit
Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components)
Your template is enriched mRNA
Your template is total RNA (poly A OR non-poly A-containing transcripts)
Kit contains oligo dT
Kit contains both oligo-dT primer and Random Hexamers
Kit contains reaction buffer with nucleotides and primer
with oligo-dT
with oligo-dT and random hexamers
You have your own first strand synthesis primer
Your template is microRNA (using your own primers)
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Available Carbohydrates, Dietary Fiber
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Limit of Detection:
0.5 g/100 g
Reaction Time (min):
~ 78 min
Application examples:
Food ingredients, food products and other materials.
The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.
All reagents stable for > 2 years after preparation
High purity / standardised enzymes employed
Only kit available
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Simple format
Document
The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
α-Amylase
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
400
Limit of Detection:
0.05 U/mL
Reproducibility (%):
~ 3%
Reaction Time (min):
~ 30 min
High purity α-Amylase HR Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
A new amylase assay reagent containing p-nitrophenyl α-D-maltoheptaoside (blocked) plus a thermostable α-glucosidase. The incorporation of this enzyme allows assays to be performed at temperatures up to 60oC and over the pH range 5.2-7.5.
High purity α-Amylase HR Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
A new amylase assay reagent containing p-nitrophenyl α-D-maltoheptaoside (blocked) plus a thermostable α-glucosidase. The incorporation of this enzyme allows assays to be performed at temperatures up to 60oC and over the pH range 5.2-7.5.
