022210P1 Lauryl Sulfate Tryptose Broth

Introduction

Usages：
Determination of coliform and fecal coliform for multiple tube fermentation.

Principle:
Tryptone provide carbon and nitrogen sources to meet the needs of bacterial growth; sodium chloride osmotic pressure balance can be maintained; Lactose is a coliform fermentable sugars; potassium dihydrogen phosphate and dipotassium phosphate is a buffer; lauryl sodium can inhibit the growth of non-coliform bacteria.

Formulation (per liter):
Trypticase:20g
Sodium chloride: 5g
Lactose:5g
Potassium dihydrogen phosphate: 2.75g
Dipotassium hydrogen phosphate :2.75g
Sodium lauryl sulfate 0.1g
Final pH6.8 ± 0.2

How to use:
1. Suspend 35.6g of the product, adding 1 L of distilled or deionized water, heated to boiling stirring until completely dissolved, packed in a test tube with a small down tube, 121 ℃ autoclave 15min, leave to cool to room temperature, standby .
2.Sample handling and dilution.
3.Selected three consecutive dilution, each dilution was inoculated three LST broth tubes, each tube was inoculated 1mL.
4. Put the tubes in an incubator 36 ± 1 ℃ cultured for 48 ± 2h.
5. Observe the results. if all LST broth don’t pruduse gas, can be reported as negative for E. coli, if gas production then will have to make further confirmed by experiments.

Quality control:
Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows:
Bacterial Name                 Bacterial No.          Growth Status       Gassing
Escherichia coli                ATCC25922               good              +

Salmonella typhimurium       CMCC (B) 50115            good             —

Staphylococcus aureus          ATCC6538                inhibited          —

Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.

Specifications: 250g/bottle

Kit Specifications
Maximum Blood Input	200 μL
Column Binding Capacity	> 50 μL
Average Yield (200 μL of blood)	2-8 μg*
Time to Complete 96 Purifications	45 minutes

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	–	–	–	–	–	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	–	–	–	12	–	–	–
Mini Spin Columns	50	100	–	–	–	–	–
Midi Spin Columns	–	–	20	–	–	–	–
96-Well Plate	–	–	–	–	2	–	2
Adhesive Tape	–	–	–	–	4	–	2
Collection Tubes	50	100	20	12	–	–	–
96-Well Collection Plate	–	–	–	–	2	–	–
Elution Tubes	50	100	20	12	–	50	–
96-Well Elution Plate	–	–	–	–	2	–	2
Product Insert	1	1	1	1	1	1	1

Clone	IHC659
Source	Mouse Monoclonal
Positive Control	Seminoma, Dysgerminoma
Dilution Range	1:200

Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications	30 minutes
Average Yield HeLa Cells (1 x 10⁶ Cells) E. coli (1 x 10⁹ cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)	15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue	100 µL150 µL1 x 10⁹ cells1 x 10⁸cells 40 mg 40 mg

Component	Cat. Dx17200 (50 preps)	Cat. Dx24300 (192 preps)	Cat. Dx24350 (192 preps)	Cat. Dx24380 (576 preps)
Lysis Solution	40 mL	–	–	–
Buffer RL	–	2 x 40 mL	2 x 40 mL	350 mL
Wash Solution	22 mL	–	–	–
Wash Solution A	22 mL	2 x 38 mL	2 x 38 mL	1 x 148 mL 1 x 74 mL
Elution Solution	6 mL	–	–	–
Elution Solution A	–	2 x 20 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	–	–	–
96-Well Isolation Plate	–	2	2	–
96-Well Isolation Plate (Deep Well)	–	–	–	6
Collection Tubes	50	–	–	–
96-Well Collection Plate	–	2	2	–
96-Well Collection Plate (Deep Well)	–	–	–	6
Elution Tubes (1.7 mL)	50	–	–	–
96-Well Elution Plate	–	2	2	–
96-Well Elution Plate (Deep Well)	–	–	–	6
Adhesive Tape	–	4	4	12
Product Insert	1	1	1	1

022210P1 Lauryl Sulfate Tryptose Broth

Detail

Introduction

Other Products

Blood DNA Isolation Kits

Overview

Details

Supporting Data

GeneAb™ SALL4

Description

Specifications

Total RNA Purification Kits Dx

Overview

Details

Supporting Data

A3P Scientific

A3P Scientific Head Office

Follow us