CRM007 Chromogenic E. coli O157

Overview

• Milk samples are stable for 1 month at room temperature (or 1 week at 37°C) in the Preservation Solution
• Fast and easy processing using a rapid spin-column format
• DNA can be isolated and detected from as little as 100 µL of milk
• Isolate high quality genomic DNA

Norgen’s Milk DNA Preservation and Isolation Kit is an all-in-one solution designed for 1) preservation of DNA in milk samples at ambient temperature; and 2) isolation of high quality DNA within a laboratory setting. The kit is provided with a Preservation Solution that allows for either immediate processing of milk samples, or allows for the storage of the milk samples for up to 1 month at room temperature prior to DNA isolation. Genomic DNA extracted from somatic cells and bacteria found in milk can be used in various applications. The isolated DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Milk DNA can also be used to diagnose particular types of infections. Milk DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis.

Milk DNA Preservative

Norgen’s Milk DNA Preservation Solution is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the preservative allow samples to be stored for 1 month without any detectable DNA degradation.

Details

Supporting Data

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Kit Specifications
Maximum Milk Input	0.5 mL
Time to Complete 10 Purifications	80 minutes (20 minutes hands on)

Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Storage in Preservation Buffer
1) Storage at -20°C is recommended for archival samples and will provide optimal preservation. The preservation buffer will freeze at -20°C. Samples can be stored indefinitely at -70°C.
2) Samples can be stored at room temperature (25°C) for up to 1 month without significant loss of DNA quality.
3) DNA has also been successfully isolated from samples stored at 37°C 1 for one week.

Kit Components	Cat. 44800 (25 preps)
Preservation Solution E	30 mL
Proteinase K	0.6 mL
Purification Additive	0.5 mL
Buffer SK	40 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Mini Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1

K-ETOH

SKU: 700004279

60 assays (manual) / 600 assays (microplate) / 600 assays (auto-analyser)

Content:	60 assays (manual) / 600 assays (microplate) / 600 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Ethanol
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	0.25 to 12 µg of ethanol per assay
Limit of Detection:	0.093 mg/L
Reaction Time (min):	~ 5 min
Application examples:	Wine, beer, cider, alcoholic fruit juices, spirits, liqueurs, low-alcoholic / non-alcoholic beverages, pickles, fruit and fruit juice, chocolate products, vinegar, jam, bread and bakery products, honey, soy sauce, dairy products, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by AOAC (AOAC Method 2019.08, First Action), IFU, EBC Method 9.3.1, MEBAK and ASBC Method Beer 4-F

The Ethanol test kit is a simple, reliable and accurate method for the measurement and analysis of ethanol in beverages and foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View our full range of alcohol assay kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• Simple format – aldehyde dehydrogenase supplied as stable suspension 
• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included
• Suitable for manual, microplate and auto-analyser formats

The Ethanol test kit is a simple, reliable and accurate method for the measurement and analysis of ethanol in beverages and foodstuffs.

NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free

Conforms to SBS and ANSI requirements

Centrifugal strength up to 3000g

Square well

50 count

Labware definition is available for immediate use in Opentron’s Labware Library

NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free

Conforms to SBS and ANSI requirements

Centrifugal strength up to 3000g

Square well

50 count

Labware definition is available for immediate use in Opentron’s Labware Library