For enrichment of bacteria of cosmetics and disposable hygiene products.
Principle:
Casein peptone and soybean peptone provide the nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; dipotassium hydrogenphosphate as a buffer; lecithin and Tween 80 can neutralize preservatives and can play a dispersed material in the emulsion features, lecithin can neutralize quaternary ammonium salt, Tween 80 neutralizes phenols, hexachlorophene, formalin, a combination that can neutralize and ethanol.
Formulation(per liter):
Casein peptone 17g
Soy peptone 3g
Sodium chloride 5g
Dipotassium hydrogen phosphate 2.5g
Glucose 2.5g
Lecithin 1g
Tween 80 7g
Final pH7.2 ± 0.2
How to use:
1.Suspend 38g in 1 L of distilled water , stirring heated to boiling until completely dissolved, distribute into flask,autoclave at 121 for 15 minutes.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
FFPE DNA Purification Kit
Product Info
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Product Info
Overview
Fast and easy processing using rapid and convenient spin-columns
Isolate high quality and high yield DNA
DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping
This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping. The protocol can be completed in as little as 1 hour.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.
Permagen’s 0.2 mL PCR Strip Magnetic rack is designed for magnetic bead separations from Individual tubes, 8-strip, or 12-strip PCR tubes
Accommodates any common PCR strips or individual PCR tubes
Tubes are angled and beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile plasma/serum input ranges
No phenol extractions
No carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Concentrate circulating RNA and exosomal RNA into a flexible elution volume
High quality, purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Compatible with Streck Cell-Free RNA BCT® Tubes
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a convenient spin column method. These kits can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications such as RT-PCR. The purified plasma/serum RNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.
Background
Plasma/Serum cell-free circulating RNA or exosomal RNA has the potential to provide biomarkers for certain cancers and disease states. Exosomes are 40 – 150 nm membrane vesicles, which are secreted by most cell types. Exosomes can be found in saliva, blood, urine, amniotic fluid and malignant ascitic fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. For this reason exosomal RNAs may serve as biomarkers for various diseases including cancer. As the RNA molecules encapsulated within exosomes are protected from degradation by RNAses they can be efficiently recovered from biological fluids, such as plasma or serum.
Plasma/Serum RNA Purification Mini Kit
This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 50 µL to 200 µL. The purified plasma/serum RNA is eluted in a flexible final volume of 10 µL to 25 µL.
Plasma/Serum RNA Purification Midi Kit
This utilizes a two column method, and can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 250 µL to 1.5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.
Plasma/Serum RNA Purification Maxi Kit
This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL.
*This kit is suitable for the isolation of RNA from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.
**Please check page 7 for Average Plasma/Serum Yields and Common RNA Quantification Methods.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
