Principle: Beef extract powder and casein hydrolyzate provide nitrogen, vitamins and amino acids; soluble starch absorption of toxic metabolites.
Formulation(per liter): Beef extract 2g Soluble starch 1.5g Acid hydrolysis of casein 17.5g Final pH7.3 ± 0.2
How to use: 1.Suspend 21g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
D6311-F-96 MagPure Blood DNA Precast Kit (96 channel machine)
Product Info
Document
Product Info
Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 200μl whole blood
Applications
PCR, southern bolt and virus detection, etc
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount
200μl
Elution volume
≥50μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
Fast – polydisperse magnetic beads, fast magnetic response and short extraction time
High purity – the obtained DNA can be directly used for second-generation sequencing, PCR based detection, gene bank, etc.
Automatic – saving time andlabor and safer
Kit Contents
Contents
D631101
D631102
D631103
Purification Times
48
96
480
MagPure Particles
1.2 ml
2.5 ml
11 ml
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
1.8 ml
5 ml
15 ml
Buffer AL
15 ml
30 ml
120 ml
Buffer BD
5 ml
10 ml
50 ml
Buffer GW1
22 ml
53 ml
220 ml
Elution Buffer
15 ml
30 ml
100 ml
Storage and Stability
Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage(up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Document
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Relevant reagents were pre-packed in 96-well plates in accordance with the optimal protocol.
CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Available for sale in Canada (Dx17200, Dx24300)
Ideal for use in in vitro diagnostic workflows
Extract high quality & quantity total RNA including miRNA
No phenol step required
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Convenient & fast spin column format
Available in 96-Well and 96-Well (Deep Well) formats for high throughput automation
Isolate from a wide variety of specimens
Purified RNA is suitable for a variety of downstream applications
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
Total RNA Purification Kit Dx (Spin Column)
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)
Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Intended Use
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
NOTE: This product is not available for sale in the United States.
Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)
15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue
100 µL150 µL1 x 109 cells1 x 108 cells 40 mg 40 mg
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
ProbeSure Master Mix combines class-leading performance with the most competitive prices in the market. ProbeSure Master Mix has provided users with accurate, robust, and consistent performance for many years. It has been carefully optimised to work over a wide range of reaction volumes and sample template qualities.
ProbeSure Master Mix is supplied at 2x concentration for convenience and is supplied with the ROX normalising dye at either high level (500 nM final concentration), low level (25 nM final concentration) or without ROX.
