Suspend 111 g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121°C for 15 minutes. Cool to 50°C and pour into sterile Petri dishes.
Formula (per liter)
Pancreatic digest of casein—————5g Pancreatic digest of animal tissue——5g Beef extract————————————1g D-mannitol————————————-10g Sodium chloride—————————–75g Agar———————————————15g Phenolred——————————–0.025g Final pH———————————–7.4±0.2
Storage
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
Saliva/Swab RNA Purification Kits
Product Info
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Product Info
Overview
Fast and easy processing using either a rapid spin column or automation friendly high-throughput 96-well plate format
Isolate total RNA, from large rRNA down to microRNA (miRNA)
Bind & elute all RNA irrespective of size or GC content, without bias
Highly sensitive & linear down to a few cells/viral copies without the need for carrier RNA
Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.
Saliva/Swab RNA Purification Kit (Spin Column)
This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.
High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
