α-Amylase HR Reagent

 Kit Storage and Term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months 

Isothermal Nucleic Acid Principle Summary

The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit Basic
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal Nucleic Acid Applications

Suitable for DNA isothermal rapid amplification kit(Basic type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

Colloidal gold probe:Require a sequence of 46-52nt in length

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Overview

• Isolate high quality, high yield plasmid DNA
• Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
• Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep

These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.

Plasmid MiniPrep Kit

This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.

Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)

Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.

Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Plasmid MaxiPrep Kit

This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.

Details

Supporting Data

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Kit Specifications
Column Binding Capacity	1.5 mg
Average Yield from 100 mL Culture*	0.4 – 1.0 mg
Final Endotoxin Levels	< 0.1 EU/µg DNA
Time to Complete 4 Purifications	1.5 hours
Size of Plasmids Purified	Up to 13,000 bp

 * Depends on high-copy DNA and low copy DNA

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.

Component	Cat. 13300 (50 preps)	Cat. 46400 (250 preps)	Cat. 46500 (4 preps)	Cat. 46600 (20 preps)	Cat. 60300 (50 preps)	Cat. 63000 (192 preps)
Resuspension Solution AZ	12 mL	60 mL	20 mL	100 mL	12 mL	2 x 20 mL
Lysis Buffer N	40 mL	80 mL	40 mL	2 x 80 mL	40 mL	2 x 40 mL
Buffer TN	20 mL	130 mL	55 mL	2 x 130 mL	20 mL	1 x 55 mL 1 x 20 mL
Wash Solution E	12 mL	2 x 18 mL	–	–	–	–
Elution Buffer K	8 mL	30 mL	–	–	8 mL	2 x 8 mL
Wash Solution J	–	–	25 mL	3 x 25 mL	–	–
Elution Buffer J	–	–	24 mL	120 mL	–	–
RNase A	1 vial	1 vial	1 vial	1 vial	1 vial	1 vial
Magnetic Bead Suspension	–	–	–	–	1 x 1.1 mL	4 x 1.1 mL
Spin Columns	50	250	–	–	–	–
Collection Tubes	50	250	–	–	–	–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)	–	–	4	20	–	–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)	–	–	4	20	–	–
96-Well Plate	–	–	–	–	–	2
Elution Tubes (1.7 mL)	50	250	–	–	50	–
Elution Tubes (50 mL)	–	–	4	20
96-Well Elution Plate	–	–	–	–	–	2
Adhesive Tape	–	–	–	–	–	2
Product Insert	1	1	1	1	1	1

K-GLULQR

SKU: 700007404

50 assays (manual) / 500 assays (auto-analyzer)

Content:	50 assays (manual) / 500 assays (auto-analyzer)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels

Analyte:	D-Glucose
Assay Format:	Spectrophotometer, Auto-analyzer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	1 to 150 µg of D-glucose per assay
Limit of Detection:	2 mg/L
Limit of Quantification:	6 mg/L
Reproducibility (%):	< 5%
Reaction Time (min):	~ 5 min

The D-Glucose (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of D-Glucose in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Browse more of our monosaccharide and oligosaccharide assay kits.

Advantages

• “Ready to use” liquid stable formulation – no reconstitution needed
• Rapid reaction (~ 5 min)
• PVP incorporated to prevent tannin inhibition
• Standard included
• Suitable for manual and auto-analyzer formats
• Quick Reference Guide available
• Mega-Calc™ software tool is available for hassle-free raw data processing 

The D-Glucose (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of D-Glucose in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.