Purchase high purity Ceralpha: α-Amylase Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
p-Nitrophenyl α-D-maltoheptaoside (blocked), plus excess α-glucosidase and glucoamylase. For the measurement of cereal, fungal and bacterial α-amylase.
View our full range of reagent mixtures.
Detail
R-CAAR4
SKU: 700005034
200 assays per kit (4 vials)
Content:
200 assays per kit (4 vials)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
α-Amylase
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
400
Limit of Detection:
0.05 U/mL
Reproducibility (%):
~ 3%
Reaction Time (min):
~ 30 min
Purchase high purity Ceralpha: α-Amylase Reagent – 4 vials for the measurement of α-amylase for research, biochemical enzyme assays and in vitro diagnostic analysis.
p-Nitrophenyl α-D-maltoheptaoside (blocked), plus excess α-glucosidase and glucoamylase. For the measurement of cereal, fungal and bacterial α-amylase.
Human Immunodeficiency Virus (HIV) TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for HIV
Available in TaqMan format for analysis
Human Immunodeficiency Virus (HIV) is a retrovirus which destroys essential cells in the human immune system, such as CD4+ lymphocytes (a type of T cell), macrophages and dendritic cells. Infection with HIV therefore leads to an impaired immune system and increased susceptibility to opportunistic cancers and infections. Due to the mutating nature of HIV and damage to the T cells, the immune system is unable to fight off infections and disease. Without treatment, HIV can progress to Acquired Immunodeficiency Syndrome (AIDS). The rate of HIV progression to AIDS depends on a number of different factors including viral, host and environmental factors. However, with adherence to antiretroviral therapy (ART), many individuals with HIV will never progress to AIDS, having near normal life expectancy. Transmission of HIV is through direct contact with infected bodily fluids, either sexually; by the sharing of blood-contaminated needles or instruments; maternally through childbirth or breastfeeding; or via blood transfusion/organ transplant.
HIV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HIV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.
All reagents stable for > 12 months after preparation
Simple format
Very specific
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Document
The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Products
RNA, miRNA
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Clinical tissues, cells, lymphocytes
Sample amount
Tissue: <20 mgCells: <5 x 106
Yield
2-50μg
Elution volume
≥30μl
Time per run
≤25 minutes
Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.
Advantages
Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – the whole extraction only takes 15-25 minutes
Nontoxic – no toxic phenol chloroform extraction is required in the extraction
Kit Contents
Contents
IVD4121
Purification Times
50 Preps
HiPure DNA Mini Column Ⅱ
50
HiPure RNA Mini Columns
50
2ml Collection Tubes
150
Proteinase K
24 mg
Protease Dissolve Buffer
1.8 ml
DNase I
600 μl
DNase Buffer
6 ml
Buffer RTL
40 ml
RNA Digestion Buffer
15 ml
Buffer RWC*
20 ml
Buffer RW2*
20 ml
Nuclease Free Water
10 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.
