【IT1000】EzRNA™ T7 High Yield RNA Synthesis Kit, 50 RXN
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The EzRNA™ T7 High Yield RNA Synthesis Kit is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.
Detail
Description
The EzRNA™ T7 High Yield RNA Synthesis Kit is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.
Features
High yield
Versatile- suitable for short and long transcripts
NTP premixed- Minimal pipetting and setup time
Compatible with CleanCap® Reagent AG
Lithium chloride included for RNA purification
Application
Generation of RNA from T7 promoter-driven DNA sequences
Suitable for subsequent cap-0 and cap-1 modification
Storage
-20°C for 12 months
Other Products
D6364 MagPure Stool DNA Kit
Product Info
Document
Product Info
Introduction
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 100-150mg stool samples
Applications
PCR, southern blot and enzyme digestion, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Stool
Sample amount
100-150 mg
Elution volume
Time per run
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Fast – several samples can be extracted in 60 minutes (after digestion)
High purity – unique adsorbent can completely remove inhibitory factors
High recovery – DNA can be recovered at the level of PG
Kit Contents
Contents
D636401
D636402
D636404
Purification Times
48 Preps
96 Preps
400 Preps
MagPure Particles N
1.7 ml
3.4 ml
14 ml
2ml Bead Tubes
48
96
400
RNase A
10 mg
20 mg
75 mg
Proteinase K
24 mg
48 mg
180 mg
Protease Dissolve Buffer
3 ml
5 ml
20 ml
Buffer ATL
60 ml
110 ml
300 ml
PVP-10
1.2 g
2.2 g
6 g
Buffer PCI
50 ml
100 ml
300 ml
Buffer MLE
30 ml
60 ml
180 ml
Buffer GW1*
22 ml
44 ml
132 ml
Elution Buffer
20 ml
20 ml
60 ml
Storage and Stability
MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
The blood bag tube sealer is a compact equipment to seal the blood bags, infusion bags, urine bags by high frequency sealing system. It is widely used in blood bank, hospital, biological industry, etc.
Features
No Hemolysis
With suction feet to avoid moving, can be used in blood collecting vehicle.
Technical Parameters
Model
HG-BTS1
Sealing time
2-4S
Sealing Type
Automatic
Diameter of tube
φ2-6mm(Tube thickness≤1.5mm)
Powe supply
220V 0.7A
Consumption
150W
External Size
310*190*150mm
Package Size
405*290*240mm
Net/Gross Weight
6/7kgs
Document
The blood bag tube sealer is a compact equipment to seal the blood bags, infusion bags, urine bags by high frequency sealing system. It is widely used in blood bank, hospital, biological industry, etc.
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from blood, buffy coat, tissue and other samples
Applications
Second generation sequencing, PCR, real time PCR, etc.
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High purity – OD 260 / 280 : 1.7- 1.9, OD 260 / 230 : 1.5 – 2.0
Economy – less than 50% of the price of Qiagen and other imported products
High yield – most optimal process, ensuring the recovery up to 90%
Strong processing ability – samples including animal blood, cultured cells, animal tissues, etc.
Kit Contents
Contents
IVD3102
Purification Times
200
MagPure Particles
5 ml
Proteinase K
100 mg
Protease Dissolve Buffer
10 ml
Rnase A
40 mg
Buffer ATL
60 ml
Buffer AL
60 ml
Buffer BD*
20 ml
Buffer BW1*
110 ml
Elution Buffer
30 ml
Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
