【IT1100】EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP), 50 RXN
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The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP (Ψ) Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.
Detail
Description
The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP (Ψ) Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit (Ψ-UTP) allows for the attainment of approximately up to 150 µg RNA yield within 2 hours at 37°C.
Features
High yield
Versatile- suitable for short and long transcripts
NTP premixed- Minimal pipetting and setup time
Compatible with CleanCap® Reagent AG
Lithium chloride included for RNA purification
Application
Generation of RNA from T7 promoter-driven DNA sequences
Suitable for subsequent cap-0 and cap-1 modification
Storage
-20°C for 12 months
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IST-129 QuickSeal Foil PCR UltraTM Self Adhesive Sealing Film
Product Info
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Product Info
Overview
Strong adhesive aluminium PCR foil seal which is pierceable, peelable and suitable for high temperature applications.
This aluminium foil seal has a strong acrylic adhesive which produces a seal of high integrity
It was developed for PCR and other high temperature applications due to its effectiveness in preventing sample evaporation
The QuickSeal Foil PCR Ultra Seal is pierceable; when pierced, the foil tears in an irregular manner which prevents the formation of a vacuum
It also has perforated end tabs for easy application and removal by peeling
For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer
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Strong adhesive aluminium PCR foil seal which is pierceable, peelable and suitable for high temperature applications.
DBCO-PEG9-DBCO is a click chemistry linker with two DBCO groups and a hydrophilic PEG spacer arm. DBCO is reactive with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG9 arm increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-PEG9-DBCO is a click chemistry linker with two DBCO groups and a hydrophilic PEG spacer arm. DBCO is reactive with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG9 arm increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed
Time Savings
Cost Efficient
High Sensitivity and Yield
Robust Enzyme
Available in 12 or 50 reaction size
Norgen’s microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.
The workflow of Norgen’s microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.
