【IT1200】EzRNA™ T7 High Yield RNA Synthesis Kit (me1Ψ-UTP), 50 RXN
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The EzRNA™ T7 High Yield RNA Synthesis Kit (me1Ψ-UTP) is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP (me1Ψ) Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit (me1Ψ-UTP) allows for the attainment of approximately up to 130 µg RNA yield within 2 hours at 37°C.
Detail
Description
The EzRNA™ T7 High Yield RNA Synthesis Kit (me1Ψ-UTP) is a user-friendly product for enzymatic RNA production. The enzyme mix contains adequate amount of T7 RNA polymerase, pyrophosphatase, and RNase inhibitors for in vitro transcription (IVT). Along with 10X Transcription Buffer and NTP (me1Ψ) Premix, users can swiftly assemble IVT reactions without compromising RNA yield. The EzRNA™ T7 High Yield RNA Synthesis Kit (me1Ψ-UTP) allows for the attainment of approximately up to 130 µg RNA yield within 2 hours at 37°C.
Features
High yieldVersatile- suitable for short and long transcriptsNTP premixed- Minimal pipetting and setup timeCompatible with CleanCap® Reagent AGLithium chloride included for RNA purification
Application
Generation of RNA from T7 promoter-driven DNA sequences
Suitable for subsequent cap-0 and cap-1 modification
Storage
-20°C for 12 months
Other Products
Urine Total RNA Purification Maxi Kit Dx (Slurry Format)
Product Info
Document
Product Info
Overview
Isolate high quality total RNA and all sizes of circulating and exosomal RNA, including microRNA
No phenol extractions
Very sensitive and linear without the need for carrier RNA
Bind and elute all RNA irrespective of size or GC content, without bias
Isolate inhibitor-free urinary RNA
Slurry/Spin column and Slurry/96-well format available
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) provides a fast, reliable and simple procedure for isolating total RNA from urine for subsequent in vitro diagnostic use. Purification is based on the use of Norgen’s proprietary resin as the separation matrix, and the kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to small RNAs.
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Urine Total RNA Purification Maxi Kit Dx (Slurry Format) in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used.
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques including RNA isolation.
Norgen’s Urine Total RNA Purification Maxi Kit Dx (Slurry Format) does not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable until the expiration date specified on the label.
Features
1.Maintenance -free brushless motor
2.LCD display of Speed, RCF and time.
3.Adjustable speed from 300r/min to 5000r/min
4.Timer from 1s to 99min or continuous operation
5.For 6x15ml centrifuge tube &10ml/7ml/5ml/3ml blood collection tube or 4x50ml centrifuge tube
6.Lid automatically open at the end of run to revent sample warming and to reduce handling time.
7.10 Program stored in memory and editable by ender user.
8.10 Acceleration/ 10 Deceleration profiles ,with soft brake system to get the good separation effect.
9.Electric cover lock, compact design, super speed and imbalance protection.
