0.2 mL PCR Strip / 1.5 mL Microcentrifuge Tube Separator
Facebook
X
Pinterest
Email
Permagen’s 0.2 mL PCR / 1.5 mL Microcentrifuge tube separation rack is designed for labs with both PCR strip and 1.5 mL separation protocols. On the top, hold up to two of either 8 or 12 strips, or up to 24 individual tubes. Flip over and hold up to twelve 1.5 mL Microcentrifuge Tubes
Detail
Permagen’s 0.2 mL PCR / 1.5 mL Microcentrifuge tube separation rack is designed for labs with both PCR strip and 1.5 mL separation protocols. On the top, hold up to two of either 8 or 12 strips, or up to 24 individual tubes. Flip over and hold up to twelve 1.5 mL Microcentrifuge Tubes
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet on both sides to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic bead
[DL1000] ExcelDye™ 6X DNA Loading Dye, Orange, 5 ml x 2
Product Info
Document
Product Info
Description
The ExcelDye™ 6× DNA Loading Dye (Orange) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration. The Orange G migrates at approximately 30 bp on a standard 2% TAE agarose gel (50 bp on 1% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.15% Orange G
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months -20°C for 36 months
Document
The ExcelDye™ 6× DNA Loading Dye (Orange) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration. The Orange G migrates at approximately 30 bp on a standard 2% TAE agarose gel (50 bp on 1% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
The iST Scientific extra volume 384-well full-skirted plate enables post-PCR applications such as enzymatic digestions and ethanol precipitation to be carried out in the wells due to the increased well volume.
Clear in colour
Polypropylene
For use in post-PCR applications such as enzymatic digestions and ethanol precipitation
384 wells
Full skirt with cut corner: A24
Packaged 10 plates per pack, 10 packs per case (100 plates total)
Document
The iST Scientific extra volume 384-well full-skirted plate enables post-PCR applications such as enzymatic digestions and ethanol precipitation to be carried out in the wells due to the increased well volume.
Efficient depletion of bovine exosomes from Fetal Bovine Serum
Deplete exosome-sized vesicles from versatile FBS volumes
No protease treatment required
No time-consuming ultracentrifugation
No precipitation reagents required
No overnight incubation required
Exosome depletion confirmed by reduction of bovine miRNAs below detectable levels
The depleted FBS provides the same cellular growth rates as the standard FBS
Purification is based on Norgen’s proprietary Silicon Carbide resin matrix
Norgen’s FBS Exosome Depletion Kits provides a quick and easy protocol for the depletion of bovine exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen’s kits allow for the processing of different FBS volumes. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, any special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for exosome depletion from FBS, as compared to the expensive current ready-to-use exosome-depleted media available on the market.
Background
Most culture medium used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth supplement to media. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of bovine exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies.
Up to 140 mL (FBS Exosome Depletion Kit I (Slurry Format) Up to 280 mL (FBS Exosome Depletion Kit II (Slurry Format)
Depletion
Deplete exosome-sized vesicle
Bovine miRNA
No detectable bovine miRNA
Time to Complete 6 Purifications
40 minutes
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
