The Permagen 0.2 mL PCR Tube Stand was designed to fit our X96 or X396 Magnet plate, however, it may also be used as a stand-alone plate for use in manual and automation applications for 0.2 mL PCR strips & tubes
SKU: X9602 (for X96)
The Permagen 0.2 mL PCR Tube Stand was designed to fit our X96 or X396 Magnet plate, however, it may also be used as a stand-alone plate for use in manual and automation applications for 0.2 mL PCR strips & tubes
Alkyne-PEG2-iodide is a crosslinker containing a propargyl group and an iodine group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. Iodine (I) is a very good leaving group for nucleophilic substitution reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
Alkyne-PEG2-iodide is a crosslinker containing a propargyl group and an iodine group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. Iodine (I) is a very good leaving group for nucleophilic substitution reactions. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research use only.
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Co-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample) |
| Applications | SDS-PAGE electrophoresis and western blot, etc |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples |
| Sample amount | Cultured cells: < 10^7Animal tissue: ≤ 20 mgPlant samples: ≤ 150 mgYeast cells: 2 x 10^6 – 5 x 10^7 |
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
Advantages
Kit Contents
| Contents | R521102 | R521103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer GW1* | 22 ml | 66 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 50 ml | 3 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
| Elution Buffer | 10 ml | 30 ml |
| Buffer ALO(5%SDS) | 10 ml | 30 ml |
Storage and Stability
HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).
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| Kit Specifications | |
| Column Binding Capacity | 25 µg |
| Average Yield:* HeLa Cells (1 x 106) Tissue (from 10 mg kidney) | 8 µg 10 µg |
| Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension | 20 mg 3 x 106 cells 150 µL 150 µL |
| Time to Complete 10 Purifications | 80 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 24700 (50 preps) | Cat. 24750 (100 preps) | Cat. 24770 (250 preps) |
|---|---|---|---|
| Digestion Buffer A | 25 mL | 2 x 25 mL | 5 x 25 mL |
| Buffer SK | 30 mL | 2 x 30 mL | 5 x 30 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 5 x 18 mL |
| Elution Buffer B | 30 mL | 2 x 30 mL | 5 x 30 mL |
| Proteinase K | 12 mg | 2 x 12 mg | 5 x 12 mg |
| Spin Columns | 50 | 100 | 250 |
| Collection Tubes | 50 | 100 | 250 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 |
| Product Insert | 1 | 1 | 1 |
