Introduction

The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 0.25ml body fluids using column and MagZol LS reagent
Applications	qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method	Mini spin column
Purification technology	Silica technology, acidphenol / guanidine extraction technology (MagZol pretreatment technology)
Process method	Manual (centrifugation or vacuum)
Sample type	Blood, serum, plasma
Sample amount	250μl
Elution volume	≥20μl
Liquid carrying volume per column	800µl
Binding yield of column	100μg

Principle

0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100 µg) binds to the membrane and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.

Advantages

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – isolation of several samples can be completed in 40 minutes by using column purification method
• Safety – no phenol chloroform extraction required
• Sensitive – direct lysis of blood, plasma and other samples without separation of leukocytes

Kit Contents

Contents	R416302	R416303
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	2 x 125
MagZol LS Reagent	60 ml	270 ml
Buffer RWC	20 ml	60 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability

MagZol LS Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The HiPure Liquid RNA & miRNA Kit integrates phenol/guanidine-based sample lysis and silica membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini spin column.

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

DD6 Floor Standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Application 

for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Widely used in the area of laboratory, pharmaceutical factory, biochemistry, biological products and so on. It is the ideal instruments for blood separation, protein precipitation and cell collection.

Features 

for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

1. Brushless DC motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.
2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.
3. Automatically electric lid lock, super speed, over temperature protection and imbalance protection.
4. Microprocessor control, speed, time, temperature and RCF in operation, speed rising and reducing quick, operate simply.
5.10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
6. There are many rotors for your choice, adapters are available by experiment requirements.
7.3 tiers protection steel cover, safe and reliable.

Technical Parameters 

for 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Max Speed: 6000r/min	Max.RCF: 6600xg
Max Volume: 4x1000ml	Power supply:AC220V,50HZ,10A
Timer: 1~99min	Noise:≤55dBA
Dimension:800×645×830mm	Net Weight: 170KG
Speed Accuracy: ±20r/min	Package(W*D*H): 920×800×1320mm
Temperature range:	Inapplicable

Matched Rotors for DD6 laboratory centrifuge

Order No.	Rotor type	Max.Speed (rpm)	Max.Volume (ml)	Max.RCF (xg)
No33097	Swing out rotor	4,000	4x1000ml (round/square cup )	4,060
No30221	Angle rotor	6,000	4x300ml	5,390
No30222	Angle rotor	6,000	6x300ml	5,660
No30223	Angle rotor	6,000	6x500ml	6,600

Packing & Shipping_____________________

by Sea/ by Air / by Express Sevices DHL, Fedex, TNT…

DD6 is floor standing large capacity 4x1000ml, 6x300ml, 6x500ml laboratory centrifuge machine without refrigerated one. It is up to max.speed 6000rpm.