Biotin-PEG3-alkyne is a biotin PEG reagent with an alkyne group that enables click reaction with azido molecules in the presence of Cu catalyst. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.

Biotin-PEG3-alkyne is a biotin PEG reagent with an alkyne group that enables click reaction with azido molecules in the presence of Cu catalyst. The hydrophilic PEG spacer arm imparts water solubility that is transferred to the biotinylated molecule.

DBCO-PEG4-Desthiobiotin is a PEG linker containing a desthiobiotin group and a DBCO functional group. Desthiobiotin is used for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while DBCO is a click chemistry handle that quickly reacts with azide groups on target molecules. Desthiobiotin is a sulfur-free analogue of biotin which binds streptavidin with slightly less strength than biotin, which provides it with a soft-release characteristic that is useful for in pull-down assays by minimizing co-elution with endogenous biotinylated molecules. The inclusion of a PEG linker in this molecule improves its aqueous solubility.

DBCO-PEG4-Desthiobiotin is a PEG linker containing a desthiobiotin group and a DBCO functional group. Desthiobiotin is used for affinity-based applications such as pull-down assays or for ligating with streptavidin proteins while DBCO is a click chemistry handle that quickly reacts with azide groups on target molecules. Desthiobiotin is a sulfur-free analogue of biotin which binds streptavidin with slightly less strength than biotin, which provides it with a soft-release characteristic that is useful for in pull-down assays by minimizing co-elution with endogenous biotinylated molecules. The inclusion of a PEG linker in this molecule improves its aqueous solubility.

Introduction

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.

Details

Specifications

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.

Advantages

Kit Contents

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.