021096P1 Sabouraud Dextrose Broth

Introduction

Usages:
For cultivations of yeasts , moulds and acidophilic bacteria.

Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.

Formulation（per liter）:
Peptone 5g
Tryptone 5g
Glucose 20g
Final pH 5.7 ± 0.1

How to use：
1.Suspend 30g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.

Quality control：
　　

Item	The name and number of strain	Growth	Colony Color
1	Aspergillus niger ATCC16404	Good	Spore surface growth
2	Candida albicans ATCC10231	Good	Cloudy broth
3	Escherichia coli ATCC25922	Inhibition	—

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle

Features	Specifications
Main Functions	Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent
Applications	RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation
Purification method	Mini spin column
Purification technology	Silica technology, DNA filtration technology
Process method	Manual (centrifugation or vacuum)
Sample type	Simple plant, petal, peel, seed
Sample amount	50-150mg
Elution volume	≥300μl
Time per run	≤15 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Contents	R401402	D401403
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
PlantZol Reagent	60 ml	200 ml
Buffer GXP	30 ml	150 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Kit Specifications
Maximum Column Binding Capacity	10 μg
Size of RNA Purified	All sizes, including small RNA (<200 nt)
Maximum Amount of Starting Material	10 μg of RNA
Minimum Elution Volume	8 μL
Time to Complete 10 Purifications	20 minutes
Average Recovery	≥ 90%

Component	Cat. 23600 (50 preps)	Cat. 43200 (100 preps)	Cat. 25100 (192 preps)	Cat. 61000 (50 preps)
Buffer RL	40 mL	2 x 40 mL	2 x 40 mL	40 mL
Wash Solution A	38 mL	2 x 38 mL	2 x 38 mL	38 mL
Elution Solution A	6 mL	2 x 6 mL	2 x 20 mL	6 mL
Column Activation Solution	–	–	–	30 mL
Micro Spin Columns	50	100	–	–
Micro-Elute RNA Spin Columns	–	–	–	50
96-Well Plate	–	–	2	–
Adhesive Tape	–	–	4	–
Collection Tubes	50	100	–	50
96-Well Collection Plate	–	–	2	–
Elution Tubes (1.7 mL)	50	100	–	50
96-Well Elution Plate	–	–	2	–
Product Insert	1	1	1	1

021096P1 Sabouraud Dextrose Broth

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