022021P1 Nutrient Agar

250g

Detail

Introduction

Usages:
For cultivating, enumerating and preserving nonfastidious bacteria.

Principle:
Peptone and beef extract powder to provide nitrogen, vitamins, amino acids and carbon; agar as medium coagulant.

Formulation(per liter):
Peptone: 5g
Beef extract :3g
Agar :15g
Final pH 6.8 ± 0.2

How to use:
1. Suspend 23g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min, set aside.
2.Diluted and treated samples.

Quality control：
　　

Item	The name and number of strain	Growth	Colony Color
1	Escherichia coli ATCC25922	Good	Dark red
2	Aerogenes CMCC (B) 45103	Good	red
3	Staphylococcus aureus ATCC6538	Suppressed	—

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Specifications: 250g/bottle

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Blood DNA Isolation Kits

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Overview

High yield and high purity DNA ready for any application
Available in a variety of formats to properly suit your needs
Compatible with blood collected on a variety of commercially available tubes

These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.

Blood DNA Isolation Mini Kit

Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.

Blood DNA Isolation Midi Kit

Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.

Blood DNA Isolation Maxi Kit

This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.

Blood DNA Isolation 96-Well Kit (High Throughput)

This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.

Blood DNA Isolation Kit (Magnetic Bead System)

Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.

Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 19

Figure 1. The difference of Ct values obtained from a Taqman qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants

Figure 1. The difference of Ct values obtained from a Taqman qPCR reaction performed on DNA isolated from blood collected on Citrate, EDTA, and Heparin anticoagulants

Figure 2. Resolution of DNA isolated from buffy coats using Norgen's Blood Genomic DNA Isolation Mini Kit

Figure 3. The difference in Ct values between DNA isolated using Norgen's Blood Genomic DNA Isolation Mini Kit (Norgen), Competitor A's DNA Blood Mini Kit (A), Competitor B's Blood Kit (B), and Competitor B's Dx Blood (B Dx), in a Taqman qPCR reaction

Figure 4. The difference in yield isolated from different volumes of blood using Norgen's vs Qiagen's kit.

Figure 5. High Yields of DNA

Figure 6. High Quality DNA

Figure 7. High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood

Figure 8. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 9. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 10. High Yields of DNA Isolated from 2 mL of Whole Blood

Figure 11. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 12. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 13. High Yields of DNA Isolated from 200 µL of Whole Blood

Figure 14. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction

Figure 15. DNA Isolated from Blood Preserved in 3 Different Anticoagulants

Figure 16. High Yield of Blood DNA

Figure 17. Detection of GAPDH from blood samples preserved in three different anticoagulants (Heparin: red, EDTA: green and Na Citrate: blue)

Figure 18. DNA Isolated from Blood Preserved in EDTA Anticoagulants

Figure 19. Detection of GAPDH From Blood Samples Preserved in EDTA Anticoagulants

Click for expanded view

Kit Specifications
Maximum Blood Input	200 µL
Average Yield (200 µL blood)*	4-12 µg
Average Purity (OD260/280)	1.8 – 1.9
Time to Complete Purifications	40 minutes (hands-on time Cat. 59800) 50 minutes (hands-on time Cat. 62600)

* Average DNA yield will vary depending on the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Component	Cat. 46300 (50 preps)	Cat. 46380 (100 preps)	Cat. 51400 (20 preps)	Cat. 31200 (12 preps)	Cat. 46350 (192 preps)	Cat. 59800 (50 preps)	Cat. 62600 (192 preps)
Lysis Buffer B	20 mL	2 x 20 mL	2 x 40 mL	2 x 110 mL	2 x 40 mL	20 mL	1 x 40 mL 1 x 20 mL
Solution WN	18 mL	2 x 18 mL	55 mL	55 mL	55 mL	18 mL	55 mL
Wash Solution A	18 mL	2 x 18 mL	2 x 38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	30 mL	2 x 30 mL	30 mL	30 mL	2 x 30 mL	15 mL	1 x 30 mL 1 x 15 mL
Proteinase K in Storage Buffer	1.2 mL	2 x 1.2 mL	4.4 mL	8 mL	4.5 mL	1.2 mL	4 mL
Magnetic Bead Suspension	–	–	–	–	–	2 x 1.1 mL	8.5 mL
Maxi Spin Columns	–	–	–	12	–	–	–
Mini Spin Columns	50	100	–	–	–	–	–
Midi Spin Columns	–	–	20	–	–	–	–
96-Well Plate	–	–	–	–	2	–	2
Adhesive Tape	–	–	–	–	4	–	2
Collection Tubes	50	100	20	12	–	–	–
96-Well Collection Plate	–	–	–	–	2	–	–
Elution Tubes	50	100	20	12	–	50	–
96-Well Elution Plate	–	–	–	–	2	–	2
Product Insert	1	1	1	1	1	1	1

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