Usages: For determination of total bacterial count.
Principle: Tryptone provide carbon and nitrogen; yeast extract powder provides B vitamins; glucose to provide energy, agar is the solidifying agent.
Formulation（per liter）: Tryptone 5.0g Yeast extract powder 2.5g Glucose 1.0g Agar 15.0g Final pH 7.0 ± 0.2
How to use： 1.Suspend 23.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Quality control：
Item
The name and number of strain
Growth
Colony
1
E.Coli ATCC8739
Good
white
2
Staphylococcus aureus ATCC6538
Good
yellow
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Goat Anti-Rabbit (GAR) Conjugated Colloidal Gold for Lateral Flow (1mL of 10OD)
Product image shows functional testing of Goat Anti Rabbit 40nm colloidal gold on a lateral flow test.
Our products are produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
Document
Goat Anti-Rabbit (GAR) Conjugated Colloidal Gold for Lateral Flow (1mL of 10OD)
Product image shows functional testing of Goat Anti Rabbit 40nm colloidal gold on a lateral flow test.
Our products are produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
The HiPure Plasmid EF Mini Kit combine the power of HiPure technology with Magen’s innovative endotoxin removal technology to deliver high-quality plasmid DNA with low endotoxin levels foruse in eukaryotic transfection, and in vitro experiments. The HiPure plasmid endo-free system uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiPure matrix. Endotoxin contamination lowers transfection efficiencies for endotoxin sensitive celllines. For gene therapy, endotoxin contamination should be of major concernsince endotoxins have the potential to cause fever, endotoxin shock syndrome, and interfere with in vitro transfection into immune cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 80µg endotoxin-free plasmid DNA from 5-15ml bacterial culture. Recommend for low copy vector, Thoroughly remove RNA
Applications
Cell transfection, animal injection, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Low copy plasmid vector
Sample amount
5-15ml LB
Yield
10-70μg
Elution volume
≥75μl
Time per run
≤40 minutes
Liquid carrying volume per column
800μl
Binding yield of column
70μg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High yield – up to 70μg plasmid can be binded in one column
Low endotoxin content – the obtained plasmid can be directly used in cell transfection and animal injection
Kit Contents
Contents
P115402
P115403
Purification Times
50 Preps
250 Preps
RNase A
5 mg
20 mg
Buffer P1
30 ml
140 ml
Buffer P2
30 ml
140 ml
Buffer LEN3
15 ml
70 ml
Buffer LN4
50 ml
250 ml
Buffer LN5
30 ml
140 ml
Buffer PW1
30 ml
140 ml
Buffer PW2
12 ml
50 ml
Elution Buffer
15 ml
30 ml
HiPure DNA Mini Columns III
50
250
2 ml Collection Tubes
50
250
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.
The HiPure Plasmid EF Mini Kit combine the power of HiPure technology with Magen’s innovative endotoxin removal technology to deliver high-quality plasmid DNA with low endotoxin levels foruse in eukaryotic transfection, and in vitro experiments. The HiPure plasmid endo-free system uses a specially formulated buffer that prevents endotoxin molecules from binding to the surface of the HiPure matrix. Endotoxin contamination lowers transfection efficiencies for endotoxin sensitive celllines. For gene therapy, endotoxin contamination should be of major concernsince endotoxins have the potential to cause fever, endotoxin shock syndrome, and interfere with in vitro transfection into immune cells.
Universal RNA Extraction Kit For High Throughput Analysis
Product Info
Document
Product Info
Product Description
Universal RNA Extraction Kit for High-Throughput Analysis
Product Description:
Amp-future Bio’s Nucleic Acid Extraction Reagent is a RNA extraction kit that provides the highest quality nucleic acid extraction. The RNA extraction kit is an automated nucleic acid extractor which allows for quick and easy RNA isolation. The reagent is in liquid form and has a shelf life of 12 months, making it a reliable and durable product. With the Nucleic Acid Extraction Reagent, Amp-future Bio provides the accuracy and reliability of a professionally designed RNA extraction kit.
Features:
Product Name: RNA Nucleic Acid Extraction Reagent
Brand: Amp-future Bio
Form: Liquid
Shelf Life: 12 Months
Application: RNA Extract
Keywords: RNA Extraction Kit
Technical Parameters:
Property
Value
Form
Liquid
Product Name
RNA Nucleic Acid Extraction Reagent
Application
RNA Extraction
Brand
Amp-future Bio
Shelf Life
12 Months
Composition
5ml*4 tubes
Applications:
Amp-future Bio’s Nucleic Acid Reagent is an advanced automated nucleic acid extraction solution designed to efficiently extract RNA from biological samples while maintaining quality and accuracy. It is made in China and has a 12-month shelf life. It comes in a liquid form and is suitable for use in automated nucleic acid extractors, allowing for automatic and highly efficient extraction of nucleic acid from various sample types. The reagent is also designed to be easy to use, making it an ideal solution for laboratories that require fast and reliable results. This reagent is a great choice for any research or clinical laboratory that needs efficient and accurate nucleic acid extraction.
Support and Services:
Technical Support and Services for Nucleic Acid Reagent We provide technical support and services for Nucleic Acid Reagents. Our experts are available to help you identify the right reagents for your application, answer any questions, and provide assistance on the use and optimization of these reagents. Our experts are also available to provide advice on troubleshooting and protocol optimization. We offer a range of services to make ordering and stocking of reagents easy and convenient.
We provide:
Expert advice on the selection of the right reagents for your application
Free samples for evaluation
Bulk and custom orders
Technical support and troubleshooting assistance
Secure and reliable online ordering
Fast and convenient shipping options
Please contact us for more information about our technical support and services for nucleic acid reagents.
Document
Amp-future Bio’s Nucleic Acid Extraction Reagent is a RNA extraction kit that provides the highest quality nucleic acid extraction. The RNA extraction kit is an automated nucleic acid extractor which allows for quick and easy RNA isolation. The reagent is in liquid form and has a shelf life of 12 months, making it a reliable and durable product. With the Nucleic Acid Extraction Reagent, Amp-future Bio provides the accuracy and reliability of a professionally designed RNA extraction kit.
