Usages: For cultivating of bacteria.And sterility test of drugs and biological products.
Principle: Tryptone, peptone and yeast extract multivalent powder provides a nitrogen source, vitamins, and growth factors; sodium chloride to maintain osmotic balance; glucose carbon source; dipotassium hydrogen phosphate as a buffering agent.
Formulation（per liter）: Pancreatic Digest of Casein 17g Papaic Digest of Soybean 3g Sodium chloride 5g Dipotassium hydrogen phosphate 2.5g Glucose Monohydrate 2.5g Final pH 7.3±0.2
How to use： 1.Suspend 30g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121℃ for 15 minutes. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Principle: Peptone provide carbon and nitrogen sources to meet the needs of bacterial growth; lactose are fermentable sugars; selenite, sodium hydrogen inhibit Gram-positive bacteria and gram-negative enterobacteria most non-Salmonella; phosphate-buffered agent; L- cystine as a reducing agent.
How to use: 1. Weigh 23g of the product , adding 1 L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing flask, cooled to room temperature . 2. Pipette 10mL of pre-enrichment sample broth or 25mL and transferred species in the liquid sample flask in a sterile environment. 3.Place into incubator, cultured at 36 ± 1 for 18-24h. 4. Observe the results.
Quality control: Quality control strains were inoculated ,and cultured at 36 ± 1 for 18-24h ,results show as follows: strain name strain code growth feature Salmonella typhi CMCC (B) 50071 good red, cloudy Salmonella typhimurium CMCC (B) 50115 good red, cloudy Escherichia coli ATCC25922 — remain unchanged
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
Solid Phase Reversible Immobilization magnetic beads are often used for DNA purification because they are simple, fast, and effective. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to nucleic acid. However, Standard SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered by SPRI beads. We have developed Magnetic Beads(microRNA & Oligo Purification) to solve the problem.
With our proprietary beads technology, the beads overcomes the hurdle of the short DNA/RNA recovery problem. The magnetic beads is ideal for microRNA purification, oligo purification, short DNA/RNA purification, and removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants effectively. The magnetic bead reagents are RNase-free and can be used for both DNA and RNA applications.
Our magnetic beads are optimized for microRNA purification, oligo purification, and DNA/RNA purification. The fragments can be as short as 20 bases, such as microRNA, tRNA, dsDNA fragments 20 bp or longer, ssDNA fragments 20 nt or longer, RNA fragments 20 nt or longer, DNA/RNA hybrid fragments 20 bp or longer, and oligos and chimeric oligos 20 nt or longer. Purified short DNA and RNA fragments are ideal for applications requiring high-quality fragments, as the fragments are free of impurities and contaminants.
Comparison of short DNA fragments recovery. BioDynami 20 bp DNA ladder (Cat. # 10002) was used as DNA input. Ampure XP beads, BioDynami beads (DNA & RNA purification) and BioDynami beads (microRNA & Oligo purification) were tested. Only BioDynami beads (microRNA & Oligo purification) successfully recovered DNA fragments between 20-100 bp.
Recovery of DNA and RNA oligos with BioDynami magnetic Beads (microRNA & Oligo Purification). 20 nt of DNA oligos and RNA oligos were used as input. Input and recovered oligos were quantified with BioDynami ssDNA Quantification kit (Cat. # 40043) and BioDynami RNA Quantification kit (Cat. # 40044).
Features
Effective purification of short DNA and RNA samples
microRNA
tRNA
dsDNA fragments 20 bp or longer
ssDNA fragments 20 nt or longer
RNA fragments 20 nt or longer
DNA/RNA hybrid fragments 20 bp or longer
Oligo and chimeric oligo 20 nt or longer
Removal of impurities and unwanted reaction components
Compatibility: Works with both manual and automated procedures
