For isolation and cultivation of Listeria monocytogenes.
Principle:
Peptone provide carbon and nitrogen sources; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride maintains osmotic equilibrium; glucose carbon source; agar as medium coagulant.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
HCM023 Fraser Broth Base
Product Info
Document
Product Info
Introduction
Intended Use
For selective enrichment culture of Listeria monocytogenes in food.
Principle and Interpretation
Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; esculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.
Formulation
Ingredients
/liter
Enzymatic digest of animal tissues ( Proteose peptone)
5.0g
Enzymatic digest of casein ( Tryptone)
5.0g
Meat extract
5.0g
Yeast extract
5.0g
Sodium chloride
20.0g
Disodium hydrogen phosphate dihydrate
12.0g
Potassium dihydrogen phosphate
1.35g
Aesculin
1.0g
Lithium chloride
3.0g
pH7.2±0.2 at 25°C
Preparation
Suspend 57.4g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃,aseptically add the contents of 1 vial of Fraser Selective Supplement (SR0120) to each 225 mL of base to prepare FB1 solution; Add one reagent (SR0130) to each100 mL of FB2 culture medium to prepare FB2 enrichment solution.
Quality Control
Cultural characteristics observed after incubation at 35-37°C for 46~50 hours
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Revision
On June 14, 2024
References
ISO 11290
Document
Intended Use For selective enrichment culture of Listeria monocytogenes in food. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen ……
CP0301J Triple Wrapped Irradiated Plate-Sabouraud Dextrose Agar
Product Info
Document
Product Info
Introduction
Usages: For monitoring and detection of surface of equipment and personnel hygiene.
Advantage: 1.This series of products was filling at A level of environment, and final sterilization by Gamma ray irradiation, triple packaging insure sterility and long shelf life. 2.Each dish was marking label product name, batch number, expiration date .Information is available of traceability. 3.Inner additional desiccant, reducing formation of condensation water, while inner additional sterile paper and plastic bags for easier transfer and cultivation. 4.Triple packing dense bags to avoid the penetration of hydrogen peroxide; clean gas was filled as a buffer to reduce broken bags and dish in transit.. 5.This series of products is available to store at (2-25 ℃), shelf life of up to 6 months.
Storage: Store in a cool (2-25 ℃), dry place, away from bright light. Storage period of 6 months.
Tumor-Associated Glycoprotein 72 (TAG-72) is a glycoprotein found on the surface of many cancer pathologies. Anti-TAG-72 can be useful for detecting some adenocarcinomas and non-neoplastic tissues. This diagnostic grade TAG-72 IVD antibody is useful for identifying adenocarcinomas in positive staining, but in mesotheliomas no staining is observed.
