027340 PYG Broth Base

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Purification and enrichment of intact cell culture media exosomes for functional studies
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents nor overnight incubation required
• Pure exosomes are purified and are free-from any other RNA-binding proteins
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Sample Type	Cell-Free Cell Culture Media
Sample Volume Range	5 ml to 10 mL
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Product Description

High Sensitivity Isothermal PCR Kit NFO | Specificity, Short Reaction Time | 30-37℃ | 10-100 CFU/reaction

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

TD5B Large Capacity Low Speed Centrifuge

Features:

1. Brushless motor with simpler construction, more reliable performance, longer life and quietly running.
2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.
4. Microprocessor control, TD5B with digital display which indicate the speed, time, RCF in operation, speed raising and reducing quick, operate simply.
5.10 kinds of accelerating and decelerating speed for your choice.

TD5B Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×800ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	685×500×385mm
Net Weight(Kg)	67KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for TD5B

Order No	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(×g)
5B-1	Swing Rotor	4000	4×800ml	3450
5B-2	Swing Rotor	4000	4×750ml	3530
5B-3	Swing Rotor	4000	4×500ml	3380
5B-4	Swing Rotor	4000	4×4×96(well)	2940
5B-5	Microplate rotor	4000	2×4×96(well)	3210
5B-6			2×3×48(well)	2300
5B-7	Swing rotor	5000	4×1×50ml	4730
			4×1×100ml	4730
		4000	4×2×100ml	3020
		4000	4×2×50ml	3200
		4000	4×4×15ml	3200
		4000	4×6×15ml	3200
5B-8	Swing rotor	4000	4×8×15ml	3200
		4000	4×12×5ml vacuum tube	2480
		4000	4×12×7ml vacuum tube	2760
		4000	4×12×10ml vacuum tube	2880
		4000	4×8×10/7/5ml vacuum tube	2790
		4000	4×6×10/7/5ml vacuum tube	2790
		4000	4×4×10/7/5ml vacuum tube	2790
5B-9	Swing rotor	4000	4×24×5ml vacuum tube	2480
		4000	4×31×5ml Khan tubes	2480
5B-10	Swing rotor	4000	4×24×7ml vacuum tube	3140
		4000	4×31×7ml Khan tubes	3140
		4000	4×30×7ml vacuum tube	3140
		4000	4×30×5ml vacuum tube	3140
		4000	4×40×7ml vacuum tube	3270
		4000	4×18×10ml vacuum tube	3140
5B-11	Swing rotor	4000	Dairy bottle	3830
5B-12	Fixed rotor	5000	6×15ml	2540
5B-13		5000	12×15ml	3080
5B-14		5000	24×15ml	3500
5B-15		5000	30×15ml	3830
5B-16		5000	4×50ml	2520
5B-17		5000	6×50ml	2850
5B-18		5000	12×50ml	3860
5B-19		4000	24×50ml	2970
5B-20		5000	4×100ml	2630
5B-21		5000	6×100ml	3130
5B-22		4000	12×100ml	2970

Adapter for 800ml and 500ml bucket

VolumeCup	5ml	7ml	10ml	15ml	50ml
800ml（Round Cup）	23pcs/cup	23	19	14	5
500ml（Round Cup）	18pcs/cup	18	14	9	3
500ml（Square Cup）	20pcs/cup	20	16	12	5

TD5B large capacity centrifuge max capacity can be reached to 4x800ml, can with different adapters for small tubes, 50ml, 15ml, 10ml, 7ml, 5ml, 2ml and so on.