DBCO-PEG10-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-PEG10-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
Details
Specifications
Features
Specifications
Main Functions
Extract total pathogen nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant.
Applications
RT-PCR，PCR
Products
Pathogen DNA / RNA
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant
Sample amount
200-300μl
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Fast – several samples can be extracted in 40 minutes by column method
High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
Safe – no phenol chloroform extraction required
Sensitive – DNA/RNA can be recovered at the level of PG
Kit Contents
Contents
IVD6672-50
IVD6672
Purification Times
50 Preps
200 Preps
Bead Tube C
50
4 x 50
MagPure Particle
1.6 ml
7.0 ml
Proteinase K
24 mg
100 mg
Protease Dissolve Buffer
1.8 ml
6 ml
Buffer MLBN
30 ml
120 ml
Buffer MW1*
22 ml
53 ml
Buffer MW2*
20 ml
50 ml
Buffer NFW
10 ml
30 ml
Storage and Stability
MagPure Particles and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
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This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
