Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Other Products
EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits
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Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact cell culture media exosomes for functional studies
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
5-20mins High Sensitivity Isothermal PCR Kit NFO Short Reaction Time
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Product Info
Product Description
High Sensitivity Isothermal PCR Kit NFO | Specificity, Short Reaction Time | 30-37℃ | 10-100 CFU/reaction
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit NFO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
1. Brushless motor with simpler construction, more reliable performance, longer life and quietly running. 2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable. 3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable. 4. Microprocessor control, TD5B with digital display which indicate the speed, time, RCF in operation, speed raising and reducing quick, operate simply. 5.10 kinds of accelerating and decelerating speed for your choice.
TD5B Technical Parameter:
Max. Speed
5000rpm
Max. RCF
4730×g
Max. Capacity
4×800ml
Time Range
0~99min
RPM/RCF Convert
Yes
Noise (dB)
≤ 58
Temperature
Normal
Acc/Dec
10 Kinds
Speed Accuracy
±20r/min
Temperature Accuracy
/
Voltage(V/Hz)
AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)
685×500×385mm
Net Weight(Kg)
67KG
Certificates
CE,ISO & Calibration report are available
Matched Rotors for TD5B
Order No
Rotor
Max Speed(r/min)
Volume(ml)
Max RCF(×g)
5B-1
Swing Rotor
4000
4×800ml
3450
5B-2
Swing Rotor
4000
4×750ml
3530
5B-3
Swing Rotor
4000
4×500ml
3380
5B-4
Swing Rotor
4000
4×4×96(well)
2940
5B-5
Microplate rotor
4000
2×4×96(well)
3210
5B-6
2×3×48(well)
2300
5B-7
Swing rotor
5000
4×1×50ml
4730
4×1×100ml
4730
4000
4×2×100ml
3020
4000
4×2×50ml
3200
4000
4×4×15ml
3200
4000
4×6×15ml
3200
5B-8
Swing rotor
4000
4×8×15ml
3200
4000
4×12×5ml vacuum tube
2480
4000
4×12×7ml vacuum tube
2760
4000
4×12×10ml vacuum tube
2880
4000
4×8×10/7/5ml vacuum tube
2790
4000
4×6×10/7/5ml vacuum tube
2790
4000
4×4×10/7/5ml vacuum tube
2790
5B-9
Swing rotor
4000
4×24×5ml vacuum tube
2480
4000
4×31×5ml Khan tubes
2480
5B-10
Swing rotor
4000
4×24×7ml vacuum tube
3140
4000
4×31×7ml Khan tubes
3140
4000
4×30×7ml vacuum tube
3140
4000
4×30×5ml vacuum tube
3140
4000
4×40×7ml vacuum tube
3270
4000
4×18×10ml vacuum tube
3140
5B-11
Swing rotor
4000
Dairy bottle
3830
5B-12
Fixed rotor
5000
6×15ml
2540
5B-13
5000
12×15ml
3080
5B-14
5000
24×15ml
3500
5B-15
5000
30×15ml
3830
5B-16
5000
4×50ml
2520
5B-17
5000
6×50ml
2850
5B-18
5000
12×50ml
3860
5B-19
4000
24×50ml
2970
5B-20
5000
4×100ml
2630
5B-21
5000
6×100ml
3130
5B-22
4000
12×100ml
2970
Adapter for 800ml and 500ml bucket
VolumeCup
5ml
7ml
10ml
15ml
50ml
800ml(Round Cup)
23pcs/cup
23
19
14
5
500ml(Round Cup)
18pcs/cup
18
14
9
3
500ml(Square Cup)
20pcs/cup
20
16
12
5
Document
TD5B large capacity centrifuge max capacity can be reached to 4x800ml, can with different adapters for small tubes, 50ml, 15ml, 10ml, 7ml, 5ml, 2ml and so on.