Overview

Clear adhesive film which uses a strong adhesive. This seal is peelable and suitable for PCR and optical applications.

• Our PCR Seal is a durable transparent polyester film with a strong adhesive layer
• The seal enables high integrity and efficiently prevents sample evaporation
• It is recommended for PCR, and other optical applications (e.g. fluorescence or colourimetric measurements) as well as sample storage
• For all adhesive seals, the best sealing results are achieved using our Hand Roller or QuickSeal A2000 Auto Sealer

Clear adhesive film which uses a strong adhesive. This seal is peelable and suitable for PCR and optical applications.

K-TDFC

SKU: 700004345

Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF

Content:	Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: Ambient, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:

Dietary Fiber

For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.

See more of our starch and dietary fiber products. 

For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.

Introduction

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 10⁷) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from yeast cultures
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Yeast culture
Sample amount	Bacterial culture: 1-1.5 ml
Elution volume	≥30μl
Time per run	≤60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).

Advantages

• Fast – several samples can be extracted in 40 minutes (after digestion)
• High purity – purified DNA can be directly used in various downstream applications
• Good repeatability – silica technology can obtain ideal results every time
• High recovery – DNA can be recovered at the level of PG
• Sufficient components – lysozyme, protease K, RNase A and glass beads

Kit Contents

Contents	D314702	D314703
Purification Times	50 Preps	250 Preps
Hipure DNA Mini Columns I	50	250
2ml Collection Tubes	50	250
Glass Beads (0.4~0.6mm)	20 g	90 g
Buffer SE	30 ml	150 ml
Lyticase	1.8 ml	5 x 1.8 ml
Buffer ATL	30 ml	150 ml
ReagentDX	500 μl	1500 μl
Buffer DL	30 ml	150 ml
Buffer GW1*	13 ml	66 ml
Buffer GW2*	20 ml	2 x 50 ml
Proteinase K	12 mg	60 mg
Protease Dissolve Buffer	1.8 ml	5 ml
Buffer AE	15 ml	30 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (