029063 Protein Toxin Crystals Stain

Description 

Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels. 

Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.

Key Features

• User-friendly gel cassette:
  • Numbered and framed wells for sample loading
  • With cassette opener for easy use
• Enhanced gel performance:
  • Enhanced band sharpness
  • Better resolution of small proteins
  • Stable for shipping at ambient temperature
• Easy compatibility: 
  • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
  • Compatible with most popular protein electrophoresis systems 

Storage and stability

Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.

Do not freeze Q-PAGE™ Precast Gels Remove tape and comb before electrophoresis.

Keep Q-PAGE™ Precast Gels flat during storage.

Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.

Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.

Cytoprep-1 bench top cytology centrifuge

Features:

1.Widely used in the field of biochemistry、biological products、pharmaceutical factory and laboratory.

2. Brushless DC motor for model Cytoprep-1, free maintenance, no powder pollution, quick in speed up and down.

3. Smooth in operation, low noise and small vibration.

4. Frame is 3 tiers protection steel jacket, and with the stainless steel chamber, inside protective steel lagging which is simple-compact, light weight.

5. Microprocessor control, it can control rotate speed, and relative RCF, digital display which indicates the speed, time, RCF.

6. Automatically electric lid lock, super speed、over temperature protection and imbalance protection.

7. Power: AC 110V/220V, 50/60HZ, speed range:0-2800rpm, speed accuracy:± 20rpm

8. Emergency locks under the bottom of the centrifuge which help you open the lid when power off.

9. The centrifuge body is made of high-quality steel, safe and reliable.

Cytoprep-1 Cytology Technical Parameters:

Max speed：	4000rpm	Max RCF：	2170×g
Max volume	6×30ml	Noise：	≤55dBA
Timer	0～99min	Net weight：	28 KG
Dimension(HxDxW)：	280×528×370mm	Power supply	AC 220V 50HZ 2A
Speed accuracy：	±20rpm	Package	carton box or wooden box

What is Collagen?

Collagen is a fundamental component of the extracellular matrix, and the predominant protein in animals, constituting around 30% of total protein mass. A glycoprotein, it is well known for its triple helical structure. This is formed from three polypeptide α-chains with Gly-X-Y repeating residues (Gly for Glycine, X for proline, and Y for hydroxyproline).

‍Types of Collagen

Over 28 types of collagens have been identified, with Type I collagen being the most abundant. It’s prevalent in ligaments, tendons, skin, and bone tissue. Its mature, insoluble form grants it remarkable strength, making it vital for the mobility of organisms. Collagen also has biochemical functions, influencing cell growth, proliferation, and differentiation.

This version of the kit is designed to detect and measure  SOLUBLE forms of collagen. Chose the Sircol Insoluble collagen kit if you need to analyse INSOLUBLE collagen.

Applications of Collagen

Collagen, with its diverse properties, finds utility in various industries. It plays a role in medicine for wound healing and has an expanding role in tissue engineering and cell culture for biomedical purposes. It’s gaining popularity in the cosmetic industry for skin rejuvenation and is used in chemical formulations and the food industry as a functional food supplement and additive.

How does Sircol 2.0 detect collagen?

The Sircol 2.0 dye reagent includes Sirius Red, a linear anionic dye with sulfonic acid side chains. This reagent is specially formulated to bind to the Gly-X-Yn helical structure of soluble collagen under assay conditions.

*The improved formulation of Sircol 2.0 dye enables a greater degree of dye-collagen specificity (compared to our previous S1000 assay kit).

‍Overview of the Sircol 2.0 assay process:

Step 1. Prepared samples are placed in the wells of the assay microplate, together with Sircol Dye Reagent. After 30 minutes mixing, any collagen-dye complexes will form as a precipitate. These are collected on the base of the microplate wells by centrifugation.

Step 2. Unbound dye is removed by gentle aspiration, followed by a rinse with Plate Wash Reagent.

Step 3. Following further centrifugation, collagen-bound dye is eluted by incubation with a Dye Release Reagent. Eluted dye is detected ‘in-situ’ by spectrophotometric analysis of the microplate at 556nm.

Step 4. The collagen content of unknown samples can be quantified by comparison against a calibration curve, prepared using the Collagen Reference Standard supplied with the kit.

A list of suggested sample types can be found under the ‘Assay Specification‘ tab.

Assay range

2 – 200µg/ml or 0.25 – 20µg/ml (using optional Collagen Concentration Protocol)

Limit of Detection

2 µg/ml

Detection Method

Colorimetric Detection (556nm) (Endpoint), Requires a microplate centrifuge.

Measurements per kit

96 in total (allows a maximum of 41 samples to be run in duplicate alongside a standard curve).

Suitable Samples

Soluble* collagens from mammalian**:

In-vivo: Tissues, cartilages and fluids.

‍In-vitro: Extracellular matrices / Conditioned media from 2D/3D culture environments.

The straightforward sample processing and analysis of Sirco 2.0 make it a good alternative to conventional hydroxyproline analysis.

‍*Prior salt/acid/acid-pepsin extraction may be necessary to release soluble collagen.

**Sircol 2.0 is primarily designed for use with in-vivo / in-vitro samples of mammalian origin. Collagens originating from other taxonomic groups and kingdoms can also be analysed. See note on p6 of manual for further information.‍

Precautions

This kit is designed for research use only. Not for use in diagnostic procedures.
Kit requires access to a microplate centrifuge* (see note below), as well as a spectrophotometer/colorimeter capable of absorbance detection at 556nm.
Specific sample preparation protocols may require customer to provide further reagents, consult assay manual for further information.

‍

*As a minimum, we recommend that the centrifuge can centrifuge a 96-well microplate at 400 x g for 120 minutes. Higher speed centrifuges are recommended (up to a maximum of 2000 x g), allowing a reduction in centrifuge time.

Sircol 2.0 kit contents:

1. Dye Reagent (1x20ml)

2. Collagen Reference Standard (1x5ml, 200µg/ml of soluble Bovine collagen)

3. Plate Wash Reagent (1x28ml)

4. Collagen Concentration Reagent (1x25ml)

5. Neutralisation Reagent (1x8ml)

6. Dye Release Reagent (1x25ml)

7. Assay Microplate (1×96-wells)

8. Microplate Seals (6x)

9. Documentation (QuickStart Guide / Manual / Certificate of Analysis)

NB: Additional reagents may be required for sample preparation prior to assay. Consult manual or contact us for further details. This kit requires the use of a microplate centrifuge, capable of centrifuging a 96-well microplate at 400 x g for 120 minutes. Higher speed centrifuges are recommended (up to a maximum of 2000 x g), allowing a reduction in centrifuge time.

Experience user-friendly detection & measurement of Soluble Collagen with Sircol™ 2.0! Our latest kit simplifies collagen quantification within in-vivo / in-vitro samples. Sircol 2.0 offers enhanced sensitivity and accuracy compared to our previous Sircol kit.