
Introduction
029080 Glycerin
Usage:Additive Reagent for Culture Media.
Specification:1ml*10vials
1ml*10vials
029080 Glycerin
Usage:Additive Reagent for Culture Media.
Specification:1ml*10vials
These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
---|---|---|---|---|---|
55000 | Plasma/Serum RNA Purification Mini Kit | 10 – 25 µL | 50 µL – 1 mL | 250 µL – 1 mL | 5 – 10 mL |
35300 | Total RNA Purification Micro Kit | 20 – 50 µL | 1 – 4 mL | 2 – 10 mL | 10 – 20 mL |
17200 | Total RNA Purification Kit | 50 – 100 µL | 4 – 10 mL | 11 – 30 mL | 20 – 35 mL |
Figure 1 / 13
Click for expanded view
Kit Specifications | |
Maximum Binding Capacity Per Well | 50 μg |
Maximum Loading Volume Per Well | 500 μL |
Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
Maximum Amount of Starting Material: | |
Animal Cells | 1 x 106 cells |
Animal Tissues | 10 mg |
Blood | 100 μL |
Plasma/Serum | 150 μL |
Bacteria | 1 x 109 cells |
Yeast | 1 x 108 cells |
Fungi | 40 mg |
Plant Tissues | 40 mg |
Time to Complete 96 Purifications | 30 minutes |
Average Yields* | |
HeLa Cells (1 x 106 cells) | 15 μg |
E. coli (1 x 109 cells) | 50 μg |
* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 17200 (50 preps) | Cat. 37500 (100 preps) | Cat. 17250 (250 preps) | Cat. 17270 (500 preps) | Cat. 24300 (192 preps) | Cat. 24370 (576 preps) | Cat. 24350 (192 preps) | Cat. 24380 (576 preps) |
---|---|---|---|---|---|---|---|---|
Buffer RL | 40 mL | 2 x 40 mL | 175 mL | 350 mL | 2 x 40 mL | 350 mL | 2 x 40 mL | 350 mL |
Wash Solution A | 38 mL | 2 x 38 mL | 148 mL | 1 x 148 mL 1 x 74 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL |
Elution Solution A | 6 mL | 2 x 6 mL | 30 mL | 60 mL | 2 x 20 mL | 60 mL | 2 x 20 mL | 60 mL |
Mini Spin Columns | 50 | 100 | 250 | 500 | – | – | – | – |
96-Well Isolation Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Isolation Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Adhesive Tape | – | – | – | – | 4 | 12 | 4 | 12 |
Collection Tubes | 50 | 100 | 250 | 500 | – | – | ||
96-Well Collection Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Collection Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Elution Tubes (1.7 mL) | 50 | 100 | 250 | 500 | – | – | ||
96-Well Elution Plate | – | – | – | – | 2 | 6 | – | – |
96-Well Elution Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
endo-BCN-PEG3-Boc-amine is a click chemistry linker containing a Boc-protected amine. The protected amine can be deprotected under mild acidic conditions. The BCN group enable copper free click chemistry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG3-Boc-amine is a click chemistry linker containing a Boc-protected amine. The protected amine can be deprotected under mild acidic conditions. The BCN group enable copper free click chemistry with azide-tagged molecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.
Cells and Tissue DNA Isolation Kit (Spin Column)
Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Micro Kit (Micro)
Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.
Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.
Figure 1 / 9
Click for expanded view
Kit Specifications | |
Maximum Cells and Tissue Input | 20 mg of animal tissue 3 x 106 cells |
Average Yield* | 8-10 µg (20 mg of animal tissue) 8-12 µg (3 x 106 cells) |
Average Purity (OD260/280) | 1.8 – 1.9 |
Time to Complete 10 Purifications | 40 minutes hands-on time (Cat. 59100) 50 minutes hands on time (Cat. 62500) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
Component | Cat. 53100 (50 preps) | Cat. 57300 (50 preps) | Cat. 59100 (50 preps) | Cat. 62500 (192 preps) |
---|---|---|---|---|
Lysis Buffer B | 20 mL | 20 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
Solution WN | 18 mL | 18 mL | 18 mL | 55 mL |
Wash Solution A | 18 mL | 18 mL | – | – |
Elution Buffer B | 30 mL | 8 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
Proteinase K | 1.2 mL | 1.2 mL | 1.2 mL | – |
Proteinase K in Sotrage Buffer | – | – | – | 4 mL |
Magnetic Bead Suspension | – | – | 2 x 1.1 mL | 8.5 mL |
96-Well Plate | – | – | – | 2 |
Spin Columns | 50 | – | – | – |
Micro Spin Columns | – | 50 | – | – |
Collection Tubes | 50 | 50 | – | – |
Elution Tubes (1.7 mL) | 50 | 50 | 50 | – |
96-Well Elution Plate | – | – | – | 2 |
Adhesive Tape | – | – | – | 2 |
Product Insert | 1 | 1 | 1 | 1 |
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