
Introduction
029100 Urea Solution,40%
Usage:Additive Reagent for Urea Agar Medium.
Specification: 2ml*10vials
2ml*1vials
029100 Urea Solution,40%
Usage:Additive Reagent for Urea Agar Medium.
Specification: 2ml*10vials
Aminooxy-PEG1-propargyl is a click chemistry PEG linker containing a propargyl group and an aminooxy group. The aminooxy group is reactive with an aldehyde or ketone group to form a stable oxime linkage. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
Aminooxy-PEG1-propargyl is a click chemistry PEG linker containing a propargyl group and an aminooxy group. The aminooxy group is reactive with an aldehyde or ketone group to form a stable oxime linkage. The propargyl group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Figure 1 / 2
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Kit Specifications | |
Maximum Binding Capacity Per Column | Up to 50 μg RNA |
Maximum Loading Volume Per Spin Column | 650 μL |
Size of RNA Purified | All sizes, including < 200 nt |
Time to Complete 10 Purifications | 25 minutes |
Maximum Amount of Starting Material | |
Liver Tissue | 20 mg |
Heart Tissue | 5 mg |
Kidney Tissue | 20 mg |
Brain Tissue | 25 mg |
Lung Tissue | 20 mg |
Spleen Tissue | 20 mg |
Whole Blood | 100 µL |
Hela Cells | 3 x 106 |
CHO | 3 x 106 |
Yeast | 1 x 108 cells |
E.coli | 1 x 109 CFU/mL |
Bacteria | 1 x 109 cells |
Plasma/Serum | 200 µL |
Fungi | 50 mg |
Plant Tissue | 50 mg |
Viruses | ≤100 µL of viral suspension |
Average Yield | |
HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 48300 (50 preps) | Cat. 48400 (100 preps) |
---|---|---|
Buffer RL | 40 mL | 2 x 40 mL |
Wash Solution A | 38 mL | 2 x 38 mL |
Elution Solution A | 6 mL | 2 x 6 mL |
RNA Purification Columns | 50 | 100 |
Genomic DNA Removal Columns | 50 | 100 |
Collection Tubes | 100 | 200 |
Elution Tubes (1.7 mL) | 50 | 100 |
Product Insert | 1 | 1 |
Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.
Figure 1 / 3
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Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Component | Cat. 61900 (192 preps) |
---|---|
Buffer SK | 30 mL |
Wash Solution A | 2 x 38 mL |
Elution Buffer B | 30 mL |
96-Well Normalization Plate | 2 |
96-Well Collection Plate | 2 |
96-Well Elution Plate | 2 |
Adhesive Tape | 1 |
Product Insert | 1 |
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