029120 Lugol Iodine

K-MBGL

SKU: 700004320

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 1 year under recommended storage conditions

Analyte:	endo-Cellulase, β-Glucanase/Lichenase
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	590
Signal Response:	Increase
Limit of Detection:	100 U/kg of malt
Reproducibility (%):	~ 7%
Reaction Time (min):	~ 35 min
Application examples:	Malt extracts, wort, beer and other materials.
Method recognition:	RACI Standard Method

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.

Looking for other assay kits? See our full range of enzyme assay kits.

Advantages

• Very cost effective 
• All reagents stable for > 2 years during use 
• Only kit available 
• Very specific 
• Simple format 
• Standard included

β-Glucanase (Malt and Microbial) Assay Kit is suitable for the measurement and analysis of malt and bacterial β-glucanase and endo-1,4-β-glucanase.

Introduction

Intended Use

For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products.

Principle and Interpretation

Pancreatic digest of gelatin provide protein, vitamins and amino acids; glucose carbon source; disodium hydrogen phosphate and potassium dihydrogen phosphate as a buffer; ox bile and brilliant green as selective antibacterial agent, inhibiting the growth of non-Enterobacteriaceae.

Formulation

Ingredients	/liter
Pancreatic digest of gelatin	10g
Glucose monohydrate	5g
Dehydrated ox bile	20g
Potassium dihydrogen phosphate	2g
Disodium hydrogen phosphate dihydrate	8g
Brilliant green	15mg
pH7.2±0.2 at 25°C

Preparation

Suspend 45g in 1L of distilled water,stir until completely dissolved and dispense 100 mL into test tubes , heat at 100 °C for 30 min in a waterbath or flowing steam.

Quality Control

The following quality control strains were inoculated and cultured at 30-35℃ for 24h-48h. The results are as follows:

Quality control strains	Inoculum (CFU)	Growth
Escherichia coli ATCC8739	< 100	Good growth
Pseudomonas aeruginosa ATCC9027
Staphylococcus aureus ATCC6538	> 100	Inhibited

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for

30 minutes.

Intended Use For enriching bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. Principle and Interpretation Pancreatic digest of gelatin pro……

Overview

Low strength adhesive film that is transparent, peelable and suitable for short term storage.

• This transparent polyester-based film has a low strength adhesive
• It is designed as a low-cost sealing option, and useful for temporary storage and as a cover for applications such as centrifugation
• End tabs allow for easy application and removal
• This seal is removable without leaving residue on the plate
• For all adhesive seals, the best sealing results are achieved using our Hand Roller or KAPS 500 Auto Sealer

Low strength adhesive film that is transparent, peelable and suitable for short term storage.