Permagen’s 12 x 1.5 mL Microfuge Tube Magnetic rack is designed for magnetic bead separations from up to 12 tubes
Detail
Permagen’s 12 x 1.5 mL Microfuge Tube Magnetic rack is designed for magnetic bead separations from up to 12 tubes
Accommodates many common 1.5 mL Microcentrifuge and some 2.0 mL tubes
Tubes are angled and beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Polydisperse silicon based magnetic beads (DNA)Monodisperse carbonyl magnetic beads (RNA)
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type
FFPE slice, FFPE puncture sample, embedded tissue
Sample amount
No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area
Yield
DNA: 1 – 10 μg, RNA: 1 – 25 μg
Principle
The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
Advantages
High quality – high purity total RNA / DNA can be directly used in various downstream applications
Safe – no phenol chloroform extraction required
Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
Post digestion sorting – higher DNA and RNA yields
High RNA yield – monodisperse carbonyl adsorption
Kit Contents
Contents
R632701
R632702
R632703
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagBind Particles
1.1 ml
2.5 ml
11 ml
MagPure Particles N
1.1 ml
2.5 ml
11 ml
Proteinase K
24 mg
48 mg
220 mg
Protease Dissolve Buffer
3 ml
10 ml
15 ml
Buffer DPS
50 ml
100 ml
2 x 250 ml
Buffer ATL
20 ml
30 ml
120 ml
Buffer BST1
20 ml
40 ml
200 ml
Buffer BXW1*
44 ml
110 ml
3 x 110 ml
RNase Free Water
15 ml
30 ml
120 ml
Storage and Stability
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.
Experiment Data
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA from skin, muscle, connective tissue, fibrous tissue sample
Applications
PCR and southern blot, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Cultured cells and tissue samples
Sample amount
Tissue: < 20mg
Principle
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Advantages
High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
Fast – column method can complete the extraction of several samples in 30 minutes
Safe – no phenol chloroform extraction required
Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
Contents
R511402
R511403
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RL
30 ml
150 ml
RNA Digestion Buffer
15 ml
80 ml
Buffer DW1
30 ml
150 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Buffer AE
10 ml
50 ml
Storage and Stability
HiPure DNA/RNA Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
Document
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.