1.5 mL 12-tube Magnetic Separation Rack

Permagen’s 15 mL / 50 mL Adapter Plate was designed with automation in mind. The SBS/ SLAS footprint (127.75mm x 85.50mm) will act as an insert on your automated liquid handler allowing any of our Centrifuge Tube racks to essentially become ready for automation

Allows the transfer from large volumes into microplates etc. where magnetic separation cannot be achieved

Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, and simple integration

SKU #

MSR1550ADP

Features

• Aluminum alloy design for years of trouble-free use
• Held to tighter tolerances for more consistent results
• SBS/ SLAS footprint (127.75 x 85.5mm) 
• Will fit into any standard robot with enough room to fit a Centrifuge tube into

Compatibility

Works with any of our six position Centrifuge Tube Racks

Permagen’s 15 mL / 50 mL Adapter Plate was designed with automation in mind. The SBS/ SLAS footprint (127.75mm x 85.50mm) will act as an insert on your automated liquid handler allowing any of our Centrifuge Tube racks to essentially become ready for automation

Name of Product

HAMA – Human Anti Mouse
Antibodies Detection Kit

Catalog Number

MQHM Z

Short Info

Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.

This product is only available in the EU!

Method/Platform

Lateral Flow

Range/Assay Sensivity

97%

Test Principle

The HAMA Detection Kit is an immunoassay designed for qualitative determination of Human Anti Mouse Antibodies in human serum.

HAMA from patient’s sample bind first to mouse antibodies which are conjugated to gold nanoparticles.

In a second step the HAMA will be caught by mouse IgG antibodies, coated on the Test Line (T) of the nitrocellulose membrane.

In the presence of the HAMA-conjugat-complex  a band becomes visible at the location of the Test Line (T).

The surplus of gold particles continues to migrate through the membrane and is captured at the Control Line (C) by specific antibodies.

Color’s intensitiy of the test line is directly proportional to the HAMA concentration in the sample.

Brief Instructions

Storage

2-8°C

Components

HAMA Test Unit, Chase Buffer, Evaluation Cards

5 tests
Lateral Flow Assay designed for the detection of Human Anti Mouse Antibodies (HAMA) in human serum.

Overview

• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
• Versatile sample input ranges
• Isolate all sizes of free-circulating RNA, including microRNA​
• Bind and elute all RNA irrespective of size or GC content, without bias
• The purified exosomal RNA is free from any circulating RNA-binding proteins
• No phenol extractions, Proteinase K treatment nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes are required
• No precipitation reagents nor overnight incubation required
• Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome and Free-Circulating RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Details

Supporting Data

Figure 1 / 6

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*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.