Permagen’s 12 x 1.5 mL Microfuge Tube Magnetic rack is designed for magnetic bead separations from up to 12 tubes
Detail
Permagen’s 12 x 1.5 mL Microfuge Tube Magnetic rack is designed for magnetic bead separations from up to 12 tubes
Accommodates many common 1.5 mL Microcentrifuge and some 2.0 mL tubes
Tubes are angled and beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by infection of various bacteria. Streptococcus dysgalactiae, a Lancefield group C α-hemolytic gram-positive bacteria, is a common pathogen in subclinical and clinical mastitis causing substantial economic losses in dairy herds. Streptococcus dysgalactiae has been isolated from infected mammary glands and teat injuries and is transmitted primarily during milking. However, detection of S. dysgalactiae in other cow tissues such as tonsils, mouth and vagina, and occurrence of S. dysgalactiae mastitis during the non-lactating period in herds, suggests that the organism also behaves as an environmental pathogen.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
Used for the selective isolation and culture of Listeria monocytogenes.
Principle and Interpretation
Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and growth factors，maintains balanced osmotic pressure; Listeria hydrolyzes aesculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.
Formulation
Ingredients
/liter
Columbia blood agar base
39.0 g
Aesculin
1.0 g
Ferric ammonium citrate
0.5 g
Lithium chloride
15.0 g
pH7.0±0.2 at 25°C
Preparation
Weigh 55.5 g of this product, add 1000 mL of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15 min. Cool to about 50℃, add 1 bottle of SR0500 supporting reagent A and 1 bottle of B per 100 mL, mix well and set aside.
Quality Control
The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:
Quality control strains
Growth
Listeria monocytogenes ATCC19115
Gray-green colonies with a black depression in the center and black surrounding
Enterococcus faecalis ATCC29212
–
Escherichia coli ATCC25922
–
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use Used for the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and……
Short term stability: Ambient, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Reaction Time (min):
~ 15 min
pH Range:
7.5-8 (recommended)
Application examples:
Dairy samples including milk, cream, whey protein, cheese, yoghurt and infant formula. Other foodstuffs such as meat, fish may also be possible.
The Carrez Clarification Kit provides easy and ready to use reagents for the removal of interfering compounds such as proteins and fats from samples, allowing improved handling and more accurate measurement of target analytes such as carbohydrates and alcohols.
This product is intended to be used as an accessory kit to the K-LOLAC, K-LACTUL, K-LACGAR, K-LATE, K-DLATE and K-FRHYD assay kits. It may also be suitable for other assay methods where sample clarification is required to remove fats or proteins before sample determination steps.
Note: this method is unsuitable for sample preparation prior to the determination of ascorbic acid (vitamin C), citrate and urea/ammonia.
Advantages
User-friendly procedure
Ready to use reagents
Allows easy sample clarification of complex matrices with high protein and fat content
Document
The Carrez Clarification Kit provides easy and ready to use reagents for the removal of interfering compounds such as proteins and fats from samples, allowing improved handling and more accurate measurement of target analytes such as carbohydrates and alcohols.
