Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
SKU: CB024
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
The Norgen UltraRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our UltraRanger contains fifteen discrete fragments ranging from 300 bp to 24000 bp with higher intensity reference bands at 5000 bp and 10000 bp. This ladder is ideal for sizing digested DNA of high molecular weight.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Figure 1 / 1
Click for expanded view
UltraRanger 1kb DNA Ladder (Cat# 12100) – 100 loads
Ladder Properties:
• Fifteen discrete fragments ranging from 300 bp to 24000 bp
• Higher intensity reference bands at 5000 bp and 10000 bp
| Fragment | Size (bp) | Mass (ng) |
| 1 | 24000 | 30 |
| 2 | 14000 | 27 |
| 3 | 10000 | 60 |
| 4 | 8000 | 44 |
| 5 | 6000 | 36 |
| 6 | 5000 | 55 |
| 7 | 4000 | 49 |
| 8 | 3000 | 39 |
| 9 | 2500 | 35 |
| 10 | 2000 | 30 |
| 11 | 1500 | 33 |
| 12 | 1000 | 10 |
| 13 | 700 | 21 |
| 14 | 500 | 18 |
| 15 | 300 | 11 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
Description
The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct PCR Master Mix Kit is a unique blend of 5X concentrated mixture containing all the essential components of a PCR reaction. The ExcelTaq™ Blood Direct PCR Master Mix Kit also contains the PCR-friendly loading and tracking dye solution Orange G. The inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is capable of tolerating the presence of PCR interfering/ inhibiting substances in blood. The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is ideal for high-throughput screening of blood samples for high reproducibility. ExcelTaq™ 5X Blood Direct DNA PCR Master Mix Kit includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.
Features
Applications
Storage
Caution: Avoid Multiple Freeze/Thaw Cycles
4°C for 6 months
-20°C for 24 months
The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct PCR Master Mix Kit is a unique blend of 5X concentrated mixture containing all the essential components of a PCR reaction. The ExcelTaq™ Blood Direct PCR Master Mix Kit also contains the PCR-friendly loading and tracking dye solution Orange G. The inclusion of Orange G tracking dye allows for a more convenient post-PCR gel electrophoresis analysis. ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is capable of tolerating the presence of PCR interfering/ inhibiting substances in blood. The ExcelTaq™ 5X Blood Direct PCR Master Mix Kit is ideal for high-throughput screening of blood samples for high reproducibility. ExcelTaq™ 5X Blood Direct DNA PCR Master Mix Kit includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.
Biotin-PEG8-DBCO is a PEG linker containing a DBCO moiety and a biotin group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal biotinylation can react with amine molecules in the presence of activator EDC or HATU. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Biotin-PEG8-DBCO is a PEG linker containing a DBCO moiety and a biotin group. The DBCO groups is commonly used for Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal biotinylation can react with amine molecules in the presence of activator EDC or HATU. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
