1.5 mL 6-tube Magnetic Separation Rack

K-GLNAM

SKU: 700004295

50 assays of each (manual) / 500 assays of each (microplate)

Content:	50 assays of each (manual) / 500 assays of each (microplate)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Ammonia, L-Glutamine
Assay Format:	Spectrophotometer, Microplate
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Decrease
Linear Range:	1 to 40 µg of L-glutamine per assay
Limit of Detection:	0.54 mg/L (L-glutamine), 0.06 mg/L (ammonia)
Reaction Time (min):	~ 10 min
Application examples:	Cell culture media and cultures, dietary supplements, vegetables and other materials (e.g. biological samples, etc.).
Method recognition:	Novel method

This product has been discontinued.

The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

See our full list of assay kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogense 
• All enzymes supplied as stabilised suspensions  
• Only enzymatic kit available 
• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.

Overview

• The preservative provides sample homogeneity eliminating sample variability
• Preserve and transport DNA & RNA safely at ambient temperature
• Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
• No cold shipping/storage needed – hassle-free and cost effective
• Isolate nucleic acids for any application including 16s NGS
• Robust preservation over a range of temperatures
• Customizable with various accessories for easy and safe collection
• Eliminates odor and renders samples safe and non-infectious

Norgen’s Stool Nucleic Acid Collection and Preservation Tubes are designed for the rapid preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended at the arrival to testing facilities until the RNA purification.

Free Download

Extracting Biological Insights from Stool

Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free

Details

Supporting Data

Figure 1 / 7

Previous

Next

Click for expanded view

Kit Specifications
Stool Input	2 g
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA 7 days for RNA*

* The RNA stability will vary depending on the samples

Storage Conditions and Product Stability

All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for 2 years after the date of shipment without any reduction in performance.

Kit Components	Cat. 45660
Stool Nucleic Acid Collection and Preservation Tubes	50
Product Insert	1

Description 

The PM1700 ExcelBand™ All Blue Broad Range Protein Marker is a blue protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 240 kDa in Tris-Glycine buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).

The PM1700 ExcelBand™ All Blue Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Two enhanced bands — 72 kDa and 25 kDa

Contents

Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control

Under suggested conditions, PM1700 ExcelBand™ All Blue Broad Range Protein Marker resolves 12 major bands in SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for long term storage