Introduction

This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.

Details

Specifications

Features	Specifications
Main Functions	Extract Pathogen RNA/DNA from 0.5ml plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for mNGS application, remove host background nucleic acid.
Applications	RT-PCR，Real Time PCR, biochip analysis, NGS
Products	Pathogen DNA / RNA
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	low/cell-free samples such as plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution
Sample amount	0.5 ml
Adaptive instrument	Nucleic acid extractor, pipetting workstation

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.

Kit Contents

Contents	R667200B	R6672-02B
Purification Times	24 Preps	96 Preps
2ml Bead Tubes	24	96
Proteinase K	12 mg	50 mg
Protease Dissolve Buffer	1.8 ml	3 ml
Buffer SDS (20%)	1.8 ml	8 ml
MagBind Particles	0.6 ml	2.5 ml
Buffer MLB	15 ml	60 ml
Buffer MW1*	13 ml	44 ml
Buffer MW2*	6 ml	50 ml
Buffer AVE	5 ml	30 ml

Storage and Stability

MagBind Particles and Proteinase K Solution should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.

This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples (including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.

Overview

• Purification from all sequence cycling by-products
• Purification of all types of enzymatic reactions
• High recovery
• Complete magnetic bead purification
• High integrity product
• Also available in a 96-well format that can be integrated with a robotic automation system
• Clean-up of amplified DNA products from enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations

Norgen’s PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) provides a rapid, simple and efficient procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on magnetic beads without the use of phenol, chloroform or alcohol precipitation.  The kit provides a high quality product with up to 90% recovery.

Norgen’s PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

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Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Component	Cat. 60200 (50 preps)	Cat. 62700 (192 preps)
Binding Buffer C	30 mL	2 x 30 mL
Elution Buffer B	8 mL	15 mL
Magnetic Bead Suspension	1.1 mL	8.5 mL
Elution Tubes (1.7 mL)	50	–
96-Well Elution Plate	–	2
Adhesive Tape	–	2
Product Insert	1	1