• For sizing and quantification of double strand DNA fragments.
• Composed of 13 bands as shown on right.
• The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.
Detail
1 kb Plus DNA Ladder in 1% agarose gel.
• For sizing and quantification of double strand DNA fragments. • Composed of 13 bands as shown on right. • The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others. • Premixed with 6X DNA loading buffer for direct gel loading.
Other Products
R6613 MagPure Blood RNA Kit Ⅱ
Product Info
Document
Product Info
Introduction
This product is suitable for extracting RNA from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents. It can specially extract high quality RNA from frozen blood samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents.
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount
1ml whole blood (fresh or frozen blood)
Principles
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR, NGS and virus detection.
Kit Contents: Bottle
Contents
R661301
R661302
R661303
Purification Times
48 Preps
96 Preps
480 preps
DNase I
600 μl
2 x 600 μl
10 x 600 µl
DNase Buffer
20 ml
30 ml
150 ml
MagPure Particles N
2.5 ml
5.0 ml
28 ml
MagZol 3BD
65 ml
140 ml
3 x 200 ml
Buffer ALB2
40 ml
60 ml
350 ml
Buffer BCP2
10 ml
15 ml
80 ml
Buffer MW2*
20 ml
50 ml
2 x 100 ml
RNase Free Water
10 ml
20 ml
60 ml
Storage and Stability
DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N, MagZol 3BD and Buffer BCP2 should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This product is suitable for extracting RNA from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents. It can specially extract high quality RNA from frozen blood samples.
ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits
Product Info
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Product Info
Overview
Columns bind proteins of interest while endotoxins flow through
Reduce endotoxin levels to less than 0.01 EU/µg of protein
Proteins are desalted
Greater than 95% protein recovery
Concentrate protein samples and remove detergents at the same time
Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.
Mini Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Maxi Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Including Triton® X-100, CHAPS, NP-40 and Tween 20
Minimum Elution Volume
50 μL
Time to Complete 10 Purifications
20 minutes
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
Component
Cat. 22800 (25 preps)
Cat. 22200 (4 preps)
Binding Buffer J
8 mL
8 mL
Binding Buffer N
4 mL
20 mL
Wash Solution M
50 mL
130 mL
Wash Solution CIP
20 mL
60 mL
Wash Solution N
30 mL
130 mL
Wash Solution NIP
20 mL
60 mL
Elution Buffer G
6 mL
20 mL
Endotoxin Removal Solution
1.5 mL
1.5 mL
Protein Neutralizer EF
2 mL
2 mL
Maxi Spin Columns (assembled with Collection Tubes)
–
4
Mini Spin Columns
25
–
Collection Tubes
25
–
Maxi Spin Columns (assembled with Collection Tubes)
Accurate Results RNA Amplification Kit High Sensitivity
Product Info
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Product Info
Product Description
RNA Amplification Kit: Accurate Results, Superior Quality
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex;perform a homology search and bind the target homology domain,at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation,the polymerase also binds to the 3′ end of the primer, initiating chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters
Details
Product Name
RNA Isothermal Amplification Kit EXO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(Fluorescent type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.