1 kb Plus DNA Ladder in Ready-to-load format.

The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.

Features

• • • Optimized condition for using with the Qubit Fluorometer
    • Uses the Qubit RNA High Sensitivity assay setting
    • Cuts costs by 60%

The performance of the BioDynami RNA Quantification HS kit is nearly identical to that of Thermo Fisher’s Qubit RNA HS kit (figure below).

Common contaminants such as detergents, solvents, salts, free nucleotides, or protein are well tolerated in the assay (Table 1).

Qubit is a registered trademark of Thermo Fisher Scientific.

The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.

TG16E Technical Parameter：

Max. Speed	16000rpm
Max. RCF	19040×g
Max. Capacity	6×10ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x D x Hmm)	370×290×215mm
Net Weight(Kg)	16KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for TG16E

Order No	Rotor	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
16E-1	Angle rotor	14000	4×8PCR	12070
16E-2	Angle Rotor	16000	12×1.5/2ml	17940
16E-3	Angle Rotor	14000	24×1.5/2ml	17950
16E-4	Angle Rotor	16000	10×5ml	17880
16E-5	Angle Rotor	13000	6×10ml	14190
16E-6	Angle Rotor	12000	24 pieces capillary vessel	15800
16E-7	Angle Rotor	16000	40×0.2ml	19040
16E-8	Angle Rotor	13000	40×0.5ml	17210
16E-9	Angle Rotor	13000	30×0.5ml	13900

Features:
1. Brushless motor, no pollution, free-maintenance.
2. Microprocessor control, LCD display which indicates the speed, time, RCF in operation, 10 kinds of brake setting, operate simply.
3. Electric lid lock, super speed, imbalance protection. The centrifuge body is made of high quality steel, stainless steel chamber, safe and reliable.
4. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
5. 3 tiers protection steel cover and get the ideal centrifugation result

Product Details

Application:

DNA Nucleic Acid

Format:

NFO Kit

Kit Components:

Reagents,Buffer,Enzymes

Manufacturer:

Amp-future Bio

Product Name:

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

Reaction Time:

5-20mins

Reaction Volume:

50μL

Sensitivity:

500-1000copies/ml

Shelf Life:

14 Months

Specificity:

High

Storage Conditions:

-20℃

Suitability:

Universal

High Light:

Isothermal DNA Amplification Kit

, 

DNA Amplification Kit NFO

, 

20mins nucleic acid amplification

Payment & Shipping Terms

Minimum Order Quantity

48T

Price

USD$3.8/T

Packaging Details

16T/Bag,48T/Box

Delivery Time

6 working days

Payment Terms

T/T, MoneyGram

Supply Ability

100000T/Month

Product Description

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

【Principle Overview】

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

【Primer design】

It is recommended to use primers with a length of 30-35 bp.Too short primers will affect the amplification speed and detection sensitivity;the 5′ end of the reverse primer is labeled with a modification group (commonly used biotin).

Primers are designed to avoid the formation of secondary structures that affect amplification; the length of the amplicon is recommended to be 150-500bp.

【Colloidal gold probe design】

In the middle of the forward and reverse primer,design a sequence of 46-52nt in length that is complementary to the target fragment; modify an antigen marker (typical FAM) at the 5′ end;mark a dSpacer (tetrahydrofuran, THF) in the middle of the 5′ end and the 3′ end ), as the recognition site of nfo;the 3′ end is labeled with a modification group, such as amine group, phosphate group or C3-Spacer, etc.

【Features】

This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 15 minutes),and the reaction components are in dry powder state,which is easy to operate and easy to store.

This reagent has low equipment requirement,and the reaction operation can be carried out in metal baths, water baths, etc.,and there is no need to purchase expensive exclusive equipment such as PCR amplifiers.

Composition	Content
A buffer	1.6mL×1Tube
B buffer	150μL×1Tube
Positive control template-II	100μL×1Tube
Positive control probe and primer mix-II	70μL×1Tube
Reagent	48 T
Guide Manual	1 Copy

【Kit storage】

1. Storage conditions: storage temperature ≤ -20℃constant temperature environment, keep away from light and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for the production date.

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.