10 Layer Cell Factory One Wide Mouth And One Narrow Mouth
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Huayi Cell Factory is a robust and easy-to-use cell
culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
10 Layer Cell Factory One Wide Mouth And One Narrow Mouth
Huayi Cell Factory is a robust and easy-to-use cell
culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.
To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.
The product assembled with ultrasonic welded technology
Versatile port design facilitates both pouring and aseptic filling techniques
Gamma radiation sterilization
the cell culture surface area
of one 10-layer Cell Factory unit is equivalent to the area
of 36 T-175 flasks
Other Products
R4144 HiPure FFPE RNA Plus Kit
Product Info
Document
Product Info
Introduction
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from FFPE tissue and section samples (with DNase)
Applications
RT-PCR, quantitative RT-PCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology, DNase
Process method
Manual (centrifugation or vacuum)
Sample type
FFPE tissue sample
Sample amount
6mg
Yield
20μg
Elution volume
≥10μl
Time per run
≤60 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis with proteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer.
Advantages
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 60 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Efficient DNA removal – unique method to effectively remove genomic DNA
Kit Contents
Contents
R414402
D414403
Purification Times
50 Preps
250 Preps
HiPure RNA Micro Columns
50
250
2ml Collection Tubes
50
250
Buffer DPS
60 ml
250 ml
Buffer FRL
15 ml
60 ml
Buffer RLC
15 ml
60 ml
Buffer RWC*
10 ml
50 ml
Buffer RW2*
20 ml
2 x 50 ml
DNase I
600 µl
5 x 600 µl
DNase Booster Buffer
1.5 ml
6 ml
Protease Dissolve Buffer
1.8 ml
10 ml
Proteinase K
24 mg
120 mg
RNase Free Water
10 ml
20 ml
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Experiment Data
Document
HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.
Excellent separation and purification of cytoplasmic and nuclear RNA
Convenient and fast spin column format
High quality and yield of RNA
Isolate full diversity of RNA (including microRNA) without phenol
Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol. Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. Ten samples can be processed in approximately 45 minutes. This kit is also available in a 100 prep size.
Background
In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA.
RNA Yield HeLa (1 x 106) – Cytoplasmic RNA HeLa (1 x 106) – Nuclear RNA
15 µg ≤ 3.5 µg
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
RNA/microRNA/DNA/Proteins are preserved for more than 2 years at room temperature in Norgen’s Urine Preservative
Compatible with most DNA, Total RNA, microRNA and protein isolation methods
Preservative is available in a single dose liquid format (ampule)
Preservative is also available in a dried format in tubes – Urine Collection and Preservation Tubes
Urine Collection and Preservation Devices are available in 4 convenient sizes: 5 cc tubes, 15 cc tubes, 50 cc tubes, and 120 cc cups
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
1. Urine Preservative Single Dose Ampules With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.
2. Urine Collection and Preservation Tubes Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:
Select Urine Tested with the Norgen Urine Collection and Preservation Tubes
Human Mouse Lynx Wolf Urine collected from snow
Available Sizes and Formats
Urine Collection and Preservation Tubes (5 cc) – Urine inputs from 1 – 5 mL
Storage Conditions and Product Stability All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
Kit Components
Cat. 18118 (50 tubes)
Cat. 18122 (50 tubes)
Cat. 18113 (50 tubes)
Cat. 18126 (50 tubes)
Cat. 18129 (1 cup)
Urine Collection and Preservation Tubes (5 cc)
50
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Urine Collection and Preservation Tubes (15 cc)
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50
–
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Urine Collection and Preservation Tubes (50 cc)
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50
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Urine Preservative Single Dose
–
–
–
50
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Urine Collection and Preservation Cup (120 cc)
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–
–
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1
ID Labels
50
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50
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Product Insert
1
1
1
1
1
Document
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
