Permagen’s Dual Volume 0.2 mL PCR Strip separator uses the same magnet configuration as our MSR812 rack above, however, when using the opposite side, the magnets are positioned towards the bottom of the tubes allowing low elution’s to be achieved. Angled tube holes allow clean area at the front of the well for clean, fast aspiration without the concern of disturbing bead pellets Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
MSRLV08
Maximum – .2 mL
Minimum – 5 µL
Permagen’s Dual Volume 0.2 mL PCR Strip separator uses the same magnet configuration as our MSR812 rack above, however, when using the opposite side, the magnets are positioned towards the bottom of the tubes allowing low elution’s to be achieved. Angled tube holes allow clean area at the front of the well for clean, fast aspiration without the concern of disturbing bead pellets Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Specifications
| Features | Specifications |
| Main Functions | Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Serum, plasma |
| Sample amount | 0.6ml |
| Elution volume | |
| Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA
| Contents | 1292750 | 12927200 |
| Purification Times | 50 preps | 200 preps |
| MagPure Particles F | 1.2 ml | 4.5 ml |
| MagBind Particles | 1.2 ml | 4.5 ml |
| Proteinase K | 24 mg | 90 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer MLB | 30 ml | 120 ml |
| Buffer GDP | 15 ml | 60 ml |
| Buffer MAW1 | 40 ml | 250 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 60 ml |
Storage and Stability
Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings