Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the udder is mainly caused by infection of various bacteria. One of such mastitis bacteria, Streptococcus agalactiae, is highly infectious and causes mainly subclinical infections, which are not identified by the herdsman. As a result, S. agalactiae can spread widely within a herd, causing immediate loss due to reduced milk. S. agalactiae is a gram-positive bacteria belonging to the Group B streptococci. Traditional cultural identification of S. agalactiae is based on S. agalactiae being beta-hemolytic as well as presence of group B Lancefield antigen and by its ability to hydrolyze sodium hippurate.
Streptococcus agalactiae TaqMan PCR Kit, 100 reactions
Streptococcus agalactiae TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM30650 (100 preps) | Cat. TM30610 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| S. agalactiae Primer & Probe Mix | 280 μL | 280 μL |
| S. agalactiae Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
DBCO-PEG2-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG2-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Opentrons Flex Deck Expansion Set (4 count) allows you to utilize up to 4 more positions on the Opentrons Flex worktable. The expansions slots are installed to the right of the worktable, acting as positions A4, B4, C4, and D4 and accepting plates, tube racks, and other labware that support SBS dimensions.
The deck expansion slots are necessary for all high-throughput workflows and can be paired with the Opentrons Flex Waste Chute to maximize the throughput capabilities of the Opentrons Flex.
The Opentrons Flex Deck Expansion Set (4 count) allows you to utilize up to 4 more positions on the Opentrons Flex worktable. The expansions slots are installed to the right of the worktable, acting as positions A4, B4, C4, and D4 and accepting plates, tube racks, and other labware that support SBS dimensions.
The deck expansion slots are necessary for all high-throughput workflows and can be paired with the Opentrons Flex Waste Chute to maximize the throughput capabilities of the Opentrons Flex.