N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl) is a multi-functional PEG linker with two terminal propargyl groups and a t-butyl ester. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The t-butyl protected carboxyl group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(t-butyl ester-PEG2)-N-bis(PEG2-propargyl) is a multi-functional PEG linker with two terminal propargyl groups and a t-butyl ester. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The t-butyl protected carboxyl group can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Synovial fluid is secreted into the joint cavity from the inner membrane of synovial joints. Synovial fluid is a plasma ultrafiltrate which contains proteins derived from both the blood plasma and produced by cells within the joint tissues. It lubricates the articulating joints as well as supplies oxygen and nutrients while removing carbon dioxide and metabolic wastes from the chondrocytes in the surrounding cartilage. Septic arthritis is usually caused by bacterial, viral or fungal infections to the synovial fluid. Staphylococcus aureus is the most common bacterial infection causing Septic arthritis. Diagnosing Septic arthritis is done through joint fluid (synovial fluid) analysis, blood tests or imaging tests.
Norgen’s Synovial Fluid Bacterial Genomic DNA Isolation Kit provides a fast, reliable and simple procedure for isolating the highest quality and the highest quantity of bacterial genomic DNA from various amounts of synovial fluid ranging from 1 mL up to 10 mL. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all Gram +ve and Gram -ve strains. Moreover, the kit allows the user to elute the purified bacterial genomic DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified bacterial gDNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis and NGS.
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| Kit Specifications | |
| Maximum Input | 2 x 109 bacterial cells |
| Column Binding Capacity | 25 μg |
| Average Yield | up to 20 µg |
| Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 67900 (50 preps) |
|---|---|
| Precipitation Solution B | 15 mL |
| Lysis Buffer T | 6.5 mL |
| Solution BX | 28 mL |
| Wash Solution A | 38 mL |
| Elution Buffer B | 8 mL |
| Micro Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Sample type purification kit guide
The ITS1 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the fungal target ITS1 libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XPMagneticBeads(supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal/swab, plasma/serum, tongue swab, gum swab, and others.
The ITS1 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the ITS1 region of the fungal DNA.The post-PCR reaction is then cleaned up using AMPure XPbeads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XPbeads. The libraries are then ready for quantification, pooling and sequencing.
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s ITS1 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components will remain stable for at least 1 year when stored at the specified storage conditions.
