Methyltetrazine-PEG5-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Methyltetrazine-PEG5-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 250-500mg soil sample |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Soil |
| Sample amount | 250-500mg |
| Elution volume | ≥50μl |
| Time per run | ≤70 minutes |
This product is based on the purification method of high binding magnetic particles. Soil sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our propietary Absorber Solution. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing buffer to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D635601 | D635602 | D635603 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles | 2.5 ml | 5 ml | 22 ml |
| 2ml Bead Tubes | 48 | 96 | 400 |
| Buffer SOL | 60 ml | 100 ml | 500 ml |
| Buffer SDS | 6 ml | 10 ml | 50 ml |
| Reagent DX | 1 ml | 1 ml | 5 ml |
| Buffer PS | 10 ml | 20 ml | 90 ml |
| Absorber Solution | 10 ml | 20 ml | 90 ml |
| Buffer GDP | 70 ml | 150 ml | 2 x 350 ml |
| Buffer GW1* | 22 ml | 44 ml | 220 ml |
| Elution Buffer | 20 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particles and Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
This product allows rapid and reliable isolation of high-quality genomic DNA from various soil, stool, and other environmental samples. Up to 500mg soil, 100mg stool, or 0.5g environmental samples can be processed in 60 minute. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
