Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
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* Yield will vary depending on the type of sample processed.
| Kit Specifications – 96-well | |
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material:FungiPlant Tissues | 40 mg40 mg |
| Average Yield* 40 mg of Grape Leaves 40 mg Tomato Leaves 40 mg Tobacco Leaves 40 mg Peach Leaves | 5-7 μg 20-30 μg 20-30 μg 15-20 μg |
| Time to Complete 96 Purifications | 30 minutes |
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Aspergillus niger
Mucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
| Component | Cat. 25800 (50 preps) | Cat. 31350 (100 preps) | Cat. 25850 (250 preps) | Cat. 31900 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer C | 60 mL | 1 x 30 mL, 1 x 60 mL | 3 x 60 mL | 2 x 60 mL |
| Wash Solution A | 38 mL | 38 mL | 1 x 18 mL, 2 x 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 6 mL | 20 mL | 20 mL |
| Filter Columns | 50 | 100 | 250 | – |
| Spin Columns | 50 | 100 | 250 | – |
| 96-Well Plate | – | – | – | 2 |
| Adhesive Tape | – | – | – | 4 |
| Collection Tubes | 100 | 200 | 500 | – |
| 96-Well Collection Plate | – | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 | – |
| 96-Well Elution Plate | – | – | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.
Background
RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 50 μg |
| Volume of Urine Processed | 1-50 mL |
| Maximum Input of Exfoliated Cells | 1 x 106 |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Time to Complete 10 Purifications | 20 minutes (for Exfoliated Cells)30 minutes (for Bacteria) |
| Average Yield | ~1μg RNA per 1 x 105 cells (For Exfoliated Cells – Varies due to cell density sample)~0.5μg RNA per 1 x 107 cells(For Bacteria – Varies due to cell density sample) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 22550 (25 preps) |
|---|---|
| Buffer SK | 15 mL |
| Wash Solution A | 18 mL |
| Elution Buffer E | 6 mL |
| Micro Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
