1000ml Tube (Low Speed)

Overview

• Detection kits for WNV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.

WNV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM44250 (100 preps)	Cat. TM44210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
WNV Primer & Probe Mix	280 μL	280 μL
WNV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

DBCO-C5-acid is an analog of DBCO-Acid with an extended 5-carbon atom spacer arm. The extended 6-carbon atom spacer arm improves its derivatization efficiency and the stability of the yielded conjugates. This spacer arm also improves solubility in organic solvents such as dichloromethane, chloroform, THF, and ethyl acetate. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.

DBCO-C5-acid is an analog of DBCO-Acid with an extended 5-carbon atom spacer arm. The extended 6-carbon atom spacer arm improves its derivatization efficiency and the stability of the yielded conjugates. This spacer arm also improves solubility in organic solvents such as dichloromethane, chloroform, THF, and ethyl acetate. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research use only.

1 kb Plus DNA Ladder in 1% agarose gel.

• For sizing and quantification of double strand DNA fragments.
• Composed of 13 bands as shown on right.
• The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.

1 kb Plus DNA Ladder in 1% agarose gel.

• For sizing and quantification of double strand DNA fragments.
• Composed of 13 bands as shown on right.
• The 10 kb and 4 kb bands with higher concentration are easily distinguishable from the others.
• Premixed with 6X DNA loading buffer for direct gel loading.