1000ml Tube (Low Speed)

NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.

100 count, 200 uL max volume per well

Labware definition is available for immediate use in Opentron’s Labware Library

NEST 96 Well Plate Flat, polystyrene, sterile, DNase, RNase, pyrogen, and endotoxin free. Individually wrapped.

100 count, 200 uL max volume per well

Labware definition is available for immediate use in Opentron’s Labware Library

Overview

• Rapid and simple procedure to generate digested peptides
• Simultaneous digestion, purification and concentration at once
• Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry
• Peptides are ready for applications such as mass spectrometry and SDS-PAGE
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format.  Trypsin is added to the protein sample and bound to the column.  Salts are washed away and the trypsin is then activated to digest proteins.  Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry.  Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation).   The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more.

Details

Supporting Data

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Kit Specifications
Maximum Protein Input	15 μg
Minimum Protein Input	2 μg
Minimum Elution Volume	30 μL
Time to Process 10 Purifications	20 minutes (Plus a 1-hour incubation)

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17500 (25 preps)
Wash Solution C	30 mL
Binding Buffer A	4 mL
Column Activation Buffer	3 mL
Micro Spin Columns	25
Collection Tubes	25
Elution tubes (1.7 mL)	25
Product Insert	1

Product Description

Amp-Future RNA Isothermal Amplification Kit,Stable & Easy To Operate At ≤-20℃

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	RNA Isothermal Amplification Kit Basic
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(Basic type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.