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The PCR Decontamination Kit can remove contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.

• The double-strand specific property of the dsDNase allows decontamination with primers and probe present.
• Efficient for end-point PCR, probe-based qPCR, and some SYBR based qPCR mixes.
• Contaminating bacterial DNA can be reduced to levels below the detection limit.
• Fast and easy protocol.
• Flat NTCs (No Template Controls).

Decontamination of master mixes without reduction of sensitivity has always been a challenge. Especially when minor amounts of DNA are targeted, contaminating DNA is a major problem. Any loss of sensitivity in the qPCR assay caused by the decontamination protocol is unacceptable.

In Figure 1, it is demonstrated that the PCR decontamination kit can remove contaminating DNA from a qPCR mix to non-detectable levels (flat NTC), without affecting the sensitivity of the qPCR.

Kit Contents

• DTT (Inactivation Aid)
• dsDNase

The PCR Decontamination Kit can remove contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.

Introduction

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots
Applications	PCR, qPCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount	Solid tissue : 1-10mg, Anticoagulant blood : 200μl
Yield	1 – 15µg
Elution volume	≥20μl
Time per run	30 – 60 minutes
Liquid carrying volume per column	750µl
Binding yield of column	100µg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast – without separation of leukocytes, organic extraction or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Wide applicability- It can handle various liquid samples, animal tissues and cultured cells

Kit Contents

Contents	IVD3018
Purification Times	100
2ml Collection Tubes	2 x 100
Buffer ATL	60 ml
Buffer AL	60 ml
Buffer GW1	44 ml
Buffer GW2	50 ml
Proteinase K	60 mg
Protease Dissolve Buffer	5 ml
Buffer AE	15 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

Articles

• 【Molecular Cancer 】Malignant clonal evolution drives multiple myeloma cellular ecological diversity and microenvironment reprogramming
• 【BIOSENSORS & BIOELECTRONICS】A multiplexed electrochemical quantitative polymerase chain reaction platform for single-base mutation analysis
• 【eLife】Domain fusion TLR2-4 enhances the autophagy-dependent clearance of Staphylococcus aureus in the genetic engineering goat
• 【TALANTA】Unique dual indexing PCR reduces chimeric contamination and improves mutation detection in cell-free DNA of pregnant women
• 【Microbiology Spectrum】Oral Bacteriome and Mycobiome across Stages of Oral Carcinogenesis | Microbiology Spectrum
• 【Biology of Sex Differences】Integrated chromatin accessibility and DNA methylation analysis to reveal the critical epigenetic modification and regulatory mechanism in gonadal differentiation of the sequentially hermaphroditic fish, Monopterus albus
• 【Cell Reports】Translocational attenuation mediated by the PERK-SRP14 axis is a protective mechanism of unfolded protein response

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

TD6 Table top laboratory centrifuge

TD6 Features:

1. Widely used in hospital, laboratory and blood bank.

2. Brushless DC motor for model TD6 which in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. The flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.

4. Micro computer control system, digital display the RCF、time and speed. There are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.

5. Electric lid lock, compact design, super speed and imbalance protection.

6. The centrifuge body is made of high-quality steel, safe and reliable.

TD6 Technical Parameters:

Max speed	6000rpm	Max RCF：	4430xg
Max volume	6x100ml	Noise：	≤55dBA
Timer	0～99min	Net weight：	32KG
Dimension(HxDxW)：	540×370×280mm	Power supply	AC 110/220V 50HZ/60hz
Speed accuracy	±20rpm	Acc/Dec	10 Kinds
Certifications	CE, ISO & Calibration report is available.	Warranty	1 Year

Matched Rotors for TD6:

Order No.	Rotor	Max Speed   (rpm)	Max Volume(ml)	Max. RCF(×g）
D6-1	Angle Rotor	6000	6×15/10/7/5ml	3660
D6-2	Angle Rotor	6000	12×15/10/7/5ml	4430
D6-3	Angle Rotor	6000	6×50ml	4110
D6-4	Angle Rotor	6000	4×50ml	3620
D6-5	Angle Rotor	6000	4×100ml	3790
D6-6	Angle Rotor	5000	24×15/10/7/5ml	3500
D6-7	Angle Rotor	4000	12×50ml	2470
D6-8	Angle Rotor	5000	6×100ml	3130
D6-9	Swing Rotor	5000	4x100ml	4420
D6-10	Swing Rotor	5000	4x50ml	4420
D6-11	Swing Rotor	5000	4x4x15ml	2820
D6-12	Swing Rotor	4000	4x6x10ml	2580
D6-13	Swing Rotor	4000	4x4x10ml	2580
D6-14	Microplate	4000	2x2x96well	2020