This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereus. Purified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.
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| Kit Specifications (96-well) | |
| Maximum Input | 2 x 109 bacterial cells |
| Well Binding Capacity | 25 µg |
| Average Yield* | Up to 20 µg* |
| Time to Complete 10 Purifications | 90 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 17900 (50 preps) | Cat. 17950 (192 preps) |
|---|---|---|
| Resuspension Solution A | 20 mL | 60 mL |
| Lysis Buffer P | 18 mL | 60 mL |
| Solution BX | 28 mL | 110 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Buffer B | 30 mL | 2 x 30 mL |
| Proteinase K | 12 mg | 50 mg |
| Spin Columns | 50 | – |
| 96-Well Plate | – | 2 |
| Adhesive Tape | – | 4 |
| Collection Plate | – | 2 |
| Collection Tubes | 50 | – |
| Elution Tubes (1.7 mL) | 50 | – |
| Elution Plate | – | 2 |
| Product Insert | 1 | 1 |
Propargyl-PEG9-alcohol is a crosslinker that can participate in copper catalyzed azide-alkyne Click Chemistry reactions to form stable triazole linkage. The PEG spacer increases the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG9-alcohol is a crosslinker that can participate in copper catalyzed azide-alkyne Click Chemistry reactions to form stable triazole linkage. The PEG spacer increases the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
