Plasmid Purification Magnetic Beads (RNA Depletion)

Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.

We have developed a simple reagent to completely remove RNA contamination in the isolated plasmid samples using Solid Phase Reversible Immobilization (SPRI) beads. SPRI beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. Our Plasmid Purification Magnetic Beads (RNA Depletion) combines BioDynami’s proprietary chemistries with the reversible DNA-binding properties of SPRI magnetic beads. The reagent removes RNA and recovers the plasmid in the same step.  Moreover, unwanted components such as salts, dNTPs, proteins, enzymes, and other impurities can also be removed simultaneously.

Plasmid can be used for downstream applications such as enzymatic digestion, transformation, transfection and molecular cloning etc.  The beads can be an effective and inexpensive reagent for bacterial RNA depletion for routine plasmid purification.

Features

• Effective depletion of bacterial RNA by RNase
• High recovery rate of plasmid DNA by magnetic beads
• Removal of unwanted components and impurities
• Simple and fast beads-based protocol

Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.

Description 

The IdPath™ COVID-19 Real-Time RT-PCR Kit is a real-time RT-PCR test intended for the detection of SARS-CoV-2 virus RNA which might be extracted from the respiratory tract specimens. The Kit provides reagents for multiplex real-time RT-PCR to detect SARS-CoV-2 by one-step reaction, specifically targeting the E (Envelope), RdRP (RNA-dependent RNA polymerase) and N (Nucleocapsid protein) gene for SARS-CoV-2 virus.

The Kit contains the RT enzyme mix and qPCR master mix for reverse transcription and real-time PCR of virus RNA.  The COVID-19 Control (positive control) and ddWater (negative control) are used as indicators to avoid false negative/positive results across all experimental procedures. The Primers/probes Mix contains multiplex primers and TaqMan probes specific to the N, E/RdRP genes of SARS-CoV-2 and RNase P gene of human, detected by FAM, VIC and ROX channels, respectively.

For Research Use Only

Features

• High Sensitivity：5×10² copies/ml (10 copies/rxn)
• High Inclusivity : >99% of currently available complete virus genomes for SARS-CoV-2 including Omicron variant
• High Accuracy : Clinical validation with 100% accuracy
• High Stability : 37/25°C for 2 weeks ; 4°C for 24 weeks ; 10 times of freeze-thaw cycles
• High Compatibility : Suitable for most laboratory qPCR machines
• Operation Control : Including internal control for quality control of total process
• Convenience : Multiplex (E/RdRP and N) detection by one-step reaction

Storage

Aliquot to avoid multiple freeze-thaw cycles

Protect fluorogenic probes from light

-20°C for 12 months

The IdPath™ COVID-19 Real-Time RT-PCR Kit is a real-time RT-PCR test intended for the detection of SARS-CoV-2 virus RNA which might be extracted from the respiratory tract specimens. The Kit provides reagents for multiplex real-time RT-PCR to detect SARS-CoV-2 by one-step reaction, specifically targeting the E (Envelope), RdRP (RNA-dependent RNA polymerase) and N (Nucleocapsid protein) gene for SARS-CoV-2 virus.
The Kit contains the RT enzyme mix and qPCR master mix for reverse transcription and real-time PCR of virus RNA.  The COVID-19 Control (positive control) and ddWater (negative control) are used as indicators to avoid false negative/positive results across all experimental procedures. The Primers/probes Mix contains multiplex primers and TaqMan probes specific to the N, E/RdRP genes of SARS-CoV-2 and RNase P gene of human, detected by FAM, VIC and ROX channels, respectively.