DBCO-PEG12-NHS Ester is a monodisperse PEG linker which enable free copper click chemistry with N3 group. NHS ester moiety can react specifically and efficiently with primary amines to form a covalent amide bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG12-NHS Ester is a monodisperse PEG linker which enable free copper click chemistry with N3 group. NHS ester moiety can react specifically and efficiently with primary amines to form a covalent amide bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Magnetic Beads (tRNA Purification)
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
The Magnetic Beads (tRNA Purification) solves the tRNA and short DNA/RNA purification problem. The beads with our proprietary technology purify tRNA effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents can be used for oligo (>70 nt) applications.
Workflow without large RNA/DNA contamination
In the case of samples contaminated with RNA/DNA such as rRNA and DNA, our magnetic beads can effectively remove RNA/DNA that are 180 nt and larger. Purified tRNA are ideal for applications requiring high quality, as the fragments are free of impurities and contaminants.
Workflow with large RNA/DNA contamination
Comparison of tRNA and 70 nt oligos recovery. BioDynami beads (tRNA purification) successfully recovered DNA fragments both yeast tRNA and 70 nt oligos.
Recovery of tRNA and 70 nt oligos with BioDynami magnetic Beads (tRNA Purification). Yeast tRNA and 70 nt oligos were used as input. Input and recovered oligos were quantified with ssDNA Quantification kit (BioDynami Cat. # 40043) and RNA Quantification kit (BioDynami Cat. # 40044).
Depletion of larger RNAs. Left panel: depletion of 28S rRNA and 18S rRNA. Right panel: depletion of RNA of 180 nt and larger.
Features
Removal of unwanted components and other impurities
Purification of tRNA and oligos (>70 nt)
tRNA
RNA fragments 70 nt or longer
DNA/RNA hybrid fragments 70 nt or longer
Oligo and chimeric oligo 70 nt or longer
dsDNA fragments 70 bp or longer
ssDNA fragments 70 nt or longer
Removal of larger RNA/DNA contamination:
18S rRNA
28S rRNA
RNA/DNA> 180 nt
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
