Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry (Lee et al., 2005). S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulasepositive, which forms the basis of traditional identification methodology.
Staphylococcus aureus TaqMan PCR Kit, 100 reactions
Staphylococcus aureus TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Component | Cat. TM29350 (100 preps) | Cat. TM29310 (100 preps) |
---|---|---|
MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
S. aureus Primer & Probe Mix | 280 μL | 280 μL |
S. aureus Positive Control | 150 μL | 150 μL |
Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
Product Insert | 1 | 1 |
K-GLOX
SKU: 700004296
200 assays (manual) / 2000 assays (microplate) / 1960 assays (auto-analyser)
Content: | 200 assays (manual) / 2000 assays (microplate) / 1960 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Glucose Oxidase |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 510 |
Signal Response: | Increase |
Linear Range: | 0.01 to 0.08 U/mL of glucose oxidase per assay |
Limit of Detection: | 10 U/L |
Reaction Time (min): | ~ 20 min |
Application examples: | Enzyme preparations, and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Novel method |
The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.
View more of our assay kits for enzyme activities.
Advantages
The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures.
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