Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.
This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.
High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.
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Kit Specifications | |
Sample Volume Range | 250 µL |
Size of RNA purified | All sizes, including small RNA (<200 nt) |
Minimum Elution Volume | 75 µL |
Maximum Elution Volume | 100 µL |
Time to complete 96 purifications | 30 minutes |
Average Yield | ≥ 1 µg **varies from sample to sample |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Kit Component | Cat. 69100 (50 preps) | Cat. 69300 (192 preps) |
---|---|---|
Lysis Buffer A | 30 mL | 100 mL |
Solution WN | 18 mL | 55 mL |
Wash Solution A | 18 mL | 2 x 38 mL |
Elution Solution A | 6 mL | 20 mL |
Mini Spin Columns | 50 | – |
Collection Tubes | 50 | – |
Elution Tubes (1.7 mL) | 50 | – |
96-Well Isolation Plate | – | 2 |
96-Well Collection Plate | – | 2 |
96-Well Elution Plate | – | 2 |
Adhesive Tape | – | 4 |
Product Insert | 1 | 1 |
Usages:
For cultivating, enumerating and preserving nonfastidious bacteria.
Principle:
Peptone and beef extract powder to provide nitrogen, vitamins, amino acids and carbon; agar as medium coagulant.
Formulation(per liter):
Peptone: 5g
Beef extract :3g
Agar :15g
Final pH 6.8 ± 0.2
How to use:
1. Suspend 23g of product, adding 1L of distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, autoclave at 121 ℃ for 15min, set aside.
2.Diluted and treated samples.
Quality control:
Item | The name and number of strain | Growth | Colony Color |
1 | Escherichia coli ATCC25922 | Good | Dark red |
2 | Aerogenes CMCC (B) 45103 | Good | red |
3 | Staphylococcus aureus ATCC6538 | Suppressed | — |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g
Clone | IHC072 |
Source | Mouse Monoclonal |
Positive Control | Lung Adenocarcinoma |
Dilution Range | 1:200 |
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