K-ETOHLQR
SKU: 700004280
60 assays (manual) / 600 assays (microplate) / 600 assays (auto-analyser)
| Content: | 60 assays (manual) / 600 assays (microplate) / 600 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 6 months under recommended storage conditions |
| Analyte: | Ethanol |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.25 to 30 µg of ethanol per assay |
| Limit of Detection: | 1.6 mg/L |
| Reaction Time (min): | ~ 7 min |
| Application examples: | Wine, beer, cider, alcoholic fruit juices, spirits, liqueurs, low-alcoholic / non-alcoholic beverages, pickles, fruit and fruit juice, chocolate products, vinegar, jam, bread and bakery products, honey, soy sauce, dairy products, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by AOAC (AOAC© Official MethodSM 2017.07) |
The Ethanol (Liquid Ready) assay kit is a rapid (~5 min), method for the specific measurement and analysis of ethanol in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual auto-analyser and microplate formats and is simple, reliable and accurate.
Suitable for manual, auto-analyser and microplate formats.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See more of our assay kits for alcohols.
Advantages
Usages:
For isolating lactose-fermenting Gram-negative enteric bacilli.
Principle:
Peptones are sources of nitrogen and other nutrients. Lactose is a fermentable carbohydrate. When lactose is fermented, alocal pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts,bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Agar is the solidifying agent.
Formulation（per liter）:
| Pancreatic Digest of Gelatin | 17.0 g |
| Peptones (meat and casein) | 3.0 g |
| Lactose Monohydrate | 10.0 g |
| Sodium Chloride | 5.0 g |
| Bile Salts | 1.5 g |
| Agar | 13.5 g |
| Neutral Red | 30.0 mg |
| Crystal Violet | 1 mg |
| Final pH | 7.1±0.2 |
How to use：
1.Suspend 50 g in 1 L of distilled or deionized water. Heat to boiling to dissolve completely. Autoclave at 121°C for 15 minutes.
2.Transfer 1 mL of Soybean–Casein Digest Broth to 100 mL of MacConkey Broth, and incubate at 42 to 44 for 24 to 48 hours. Subculture on a plate of MacConkey Agar at 30 to 35 deg.C for 18 to 72 hours.
Quality control：
| Item | The name and number of strain | PR/G | Reaction |
| Growth rate | E.Coli ATCC8739 | PR≥0.7 | Rose-red |
| Characteristic difference | Proteus mirabilis CMCC(b)49005 | PR≥0.7 | Colorless, no swarming |
| Selective | Staphylococcus aureus ATCC6538 | G≤1 | － |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of three years.
Specifications: 250g/bottle
250g
DBCO-PEG1-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG1-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.