Introduction
This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 5ml plasma, serum, body fluids |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Serum, plasma |
| Sample amount | 5ml |
| Elution volume | ≥40μl |
| Time per run | ≤50 minutes |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.
Advantages
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – without labour
Kit Contents
| Contents | 1292750 | 12927200 |
| Purification Times | 50 | 200 |
| MagPure Particles G | 20 ml | 80 ml |
| MagBind Particles (selection particles) | 14 ml | 58 ml |
| Selection Solution | 100 ml | 400 ml |
| Proteinase K | 300 mg | 1.2 g |
| Protease Dissolve Buffer | 25 ml | 100 ml |
| Buffer SDS(20%) | 15 ml | 60 ml |
| Buffer MLK | 300 ml | 3 x 450 ml |
| Buffer BST1 | 225 ml | 2x 450 ml |
| Buffer MKW1 | 225 ml | 2x 450 ml |
| Buffer MW2* | 50 ml | 2x 100 ml |
| Buffer AE | 10 ml | 30 ml |
Storage and Stability
MagPure Particles G, MagBind Particles and Proteinase K should bestored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at roomtemperature (15–25°C) and are stable for at least 18 months underthese conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
