The Norgen PCR Ranger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCR Ranger DNA Ladder contains eleven discrete fragments ranging from 50 bp to 1000 bp with a higher intensity reference band at 500 bp. This Ladder is ideal for assessment of a range of PCR product sizes.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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PCR Ranger 100 bp DNA Ladder (Cat# 11300) – 100 loads
Ladder Properties:
• Eleven discrete bands, ranging from 50 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference
Fragment | Size (bp) | Mass (ng) |
1 | 1000 | 91 |
2 | 900 | 66 |
3 | 800 | 61 |
4 | 700 | 51 |
5 | 600 | 43 |
6 | 500 | 70 |
7 | 400 | 28 |
8 | 300 | 23 |
9 | 200 | 30 |
10 | 100 | 22 |
11 | 50 | 15 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
Features | Specifications |
Main Functions | Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture. Recommend for High copy vector, Low elution volume, High concentration |
Applications | Cell transfection, animal injection, etc. |
Purification method | Midi spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | High copy plasmid vector |
Sample amount | 25-50ml LB |
Yield | 10-500μg |
Elution volume | ≥0.1ml |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 250μg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
Kit Contents
Contents | P123102 | P123102B |
Purification Times | 50 Preps | 50 Preps |
RNase A | 30 mg | 30 mg |
Buffer E1 | 150 ml | 150 ml |
Buffer E2 | 150 ml | 150 ml |
Buffer E3 | 150 ml | 150 ml |
Buffer E4 | 150 ml | 150 ml |
Buffer E5 | 150 ml | 150 ml |
Buffer PW2* | 50 ml | 50 ml |
Elution Buffer | 30 ml | 30 ml |
MaxPure EF Mini Column | 50 | 50 |
2 ml Collection Tubes | 50 | 50 |
Lysate Clear Midi Syringe | 50 | 50 |
Extend Tubes | 50 | 50 |
50ml Centrifuge Tubes | 50 | |
Support Tubes | 50 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2–8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from <10 mg insect tissue |
Applications | PCR, southern bolt and virus detection, etc |
Purification method | Midi spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Insect tissue samples |
Sample amount | <10 mg |
Elution volume | ≥15μl |
Time per run | ≤30 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Contents | D312902 | D312903 |
Purification Times | 50 | 250 |
HiPure DNA Mini Columns I | 50 | 250 |
2ml Collection Tubes | 50 | 250 |
Buffer ITL | 30 ml | 120 ml |
Buffer IL* | 30 ml | 120 ml |
Buffer GW1* | 22 ml | 110 ml |
Buffer GW2* | 20 ml | 2 x 50 ml |
Proteinase K | 24 mg | 120 mg |
Protease Dissolve Buffer | 1.8 ml | 15 ml |
Buffer AE | 15 ml | 60 ml |
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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