This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from tissue / blood / body fluid / swab /dry blood spots |
| Applications | PCR, qPCR, southern bolt and virus detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples |
| Sample amount | Solid tissue : 1-10mg, Anticoagulant blood : 200µl |
| Yield | 1 – 15µg |
| Elution volume | ≥20μl |
| Time per run | 30 – 60 minutes |
| Liquid carrying volume per column | 750µl |
| Binding yield of column | 100µg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | IVD3018 |
| Purification Times | 100 |
| HiPure DNA Mini Columns I | 100 |
| 2ml Collection Tubes | 2 x 100 |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer GW1 | 44 ml |
| Buffer GW2 | 50 ml |
| Proteinase K | 60 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer AE | 15 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
| Contents | D312402 | D312403 |
| Purification Times | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Buffer BGL | 40 ml | 180 ml |
| Buffer GXP | 30 ml | 150 ml |
| Buffer GW1* | 13 ml | 53 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| DTT Powder | 235 mg | 2 x 235 mg |
| Proteinase K | 48 mg | 240 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Elution Buffer | 15 ml | 60 ml |
Storage and Stability
This product can be stored at room temperature (15~25°C) for 18 months. Proteinase K/DTT dry powder can be transported and stored at room temperature. For long-term storage (>6 months), it is recommended to store at -20~8°C. Dissolved Proteinase K should be stored at -20~8°C. The dissolved DTT should be stored at -20°C.
This product provides fast and easy method for purification of high purity DNA from bone samples. The obtained DNA can be directly used for PCR and STR detection down stream application.
N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.