Permagen’s 0.2 mL PCR Strip Magnetic rack is designed for magnetic bead separations from Individual tubes, 8-strip, or 12-strip PCR tubes
Accommodates any common PCR strips or individual PCR tubes
Tubes are angled and beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
MSR812
Maximum – .2 mL
Minimum – 30 µL
Permagen’s 0.2 mL PCR Strip Magnetic rack is designed for magnetic bead separations from Individual tubes, 8-strip, or 12-strip PCR tubes
K-PullG6
SKU: 700004329
100/200 assays per kit
Content: | 100 / 200 assays per kit |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Pullulanase/Limit-Dextrinase |
Assay Format: | Spectrophotometer |
Detection Method: | Absorbance |
Wavelength (nm): | 400 |
Signal Response: | Increase |
Limit of Detection: | 0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt |
Reproducibility (%): | ~ 3% |
Total Assay Time: | ~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase) |
Application examples: | Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts. |
Method recognition: | Novel method |
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
Explore more of our assay kit products for enzyme activity measurement.
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