DBCO-PEG5-DBCO is a bifunctional click chemistry reagent. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG5-DBCO is a bifunctional click chemistry reagent. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-GCROL
SKU: 700004292
70 assays (manual) / 700 assays (microplate)
Content: | 70 assays (manual) / 700 assays (microplate) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Glycerol |
Assay Format: | Spectrophotometer, Microplate |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Decrease |
Linear Range: | 0.8 to 35 µg of glycerol per assay |
Limit of Detection: | 0.34 mg/L |
Reaction Time (min): | ~ 5 min |
Application examples: | Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices, soft drinks, toothpaste, honey, tobacco, paper (and cardboard), cosmetics, pharmaceuticals, soap and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Methods based on this principle have been accepted by OIV and MEBAK |
The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Browse our wide range of alcohol assay kit products.
Advantages
The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 150 mg plant and fungal tissue |
Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Fresh / frozen plant samples, dried plant / seed samples |
Sample amount | Fresh / frozen plant samples: 50-150 mgDried plant / seed samples: 15-40 mg |
Elution volume | ≥40μl |
Time per run | 30-50 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Kit Contents
Contents | D318702 | D318703 |
Purification Times | 50 Preps | 250 Preps |
Buffer PAL | 60 ml | 200 ml |
Buffer GWP | 60 ml | 200 ml |
Buffer DW1 | 30 ml | 150 ml |
Buffer GW2* | 20 ml | 50 ml |
Buffer AE | 20 ml | 60 ml |
HiPure DNA Mini Columns II | 50 | 250 |
2 ml Collection Tubes | 50 | 250 |
Storage and Stability
This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Name | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Elution volume | Yield (muscle) |
HiPure HP Plant DNA Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100μl | 3-70μg |
HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250μg |
HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100μl | 3-50μg |
HiPure SF Plant DNA 96 Kit | D3167 | 50mg | 15mg | 96 well plate | 75 – 150μl | 3-30μg |
HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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