HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.5g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA |
| Applications | PCR, NGS, labeling, ligation and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Agarose gel, PCR products, enzyme products |
| Sample amount | Agarose gel: ≤500mg |
| Recovery | ≥80% |
| Elution volume | ≥15μl |
| Time per run | ≤20 minutes（1-24 samples） |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 35µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D211102 | D211103 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer GDP | 120 ml | 250 ml |
| Buffer DW2 | 50 ml | 2 x 50 ml |
| Elution Buffer | 20 ml | 30 ml |
| HiPure DNA Mini Columns II | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Experiment Data
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
SKU: CB024
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
Usage: For isolating lactose-fermenting Gram-negative enteric bacilli.
Approximative Formula(Per Liter)
Pancreatic Digest of Gelatin 17g
Peptones(meat and casein) 3.0g
Lactose Monohydrate 10g
Sodium Chloride 5g
Bile Salts 1.5g
Agar 13.5g
Neutral Red 30mg
Crystal Violet 1mg
Final pH 7.1±0.2
Directions: Suspend 50g in 1 L of distilled or deionized water. Heat with frequent agitation to dissolve. Boil 1~2min. Distribute into flasks. Cool to 45~50°C and pour into sterile petri dishes.
Storage: Keep container tightly closed, store in a dry place, away from bright light.
500g