Attogene has developed a set of RNase-Free Lateral Assay Buffers specifically designed for developers of Nucleic Acid-based lateral flow tests
Components
Set of 5 RNase-Free Lateral flow running buffers at specifically developed for optimizing nucleic acid based lateral flow assays. Each buffer is certified RNase-Free and tested functionally in RNA and DNA based lateral flow tests.
Features & Benefits
5 bottles of Running Buffer (10mL)
SKU: AU2035
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.
It is special designed for Pathogen RNA extraction from Sputum samples, can be used in tuberculosis (TB) detection.
Specifications
| Features | Specifications |
| Main Function | Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection. |
| Applications | RT-PCR，PCR |
| Products | Pathogen RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant, sputum |
| Sample amount | 200-300μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Kit Contents
| Contents | IVD6672C |
| Purification Times | 200 Preps |
| 2ml Bead Tube | 4 x 50 |
| MagPure Particle | 7.0 ml |
| Proteinase K | 100 mg |
| Protease Dissolve Buffer | 6 ml |
| DTT （Powder) | 2g |
| Buffer SDS | 15 ml |
| Buffer MLBN | 220 ml |
| DNase I | 4 x 0.6 ml |
| DNase Buffer | 60 ml |
| Buffer MW1* | 53 ml |
| Buffer MW2* | 50 ml |
| Buffer NFW | 30 ml |
Storage and Stability
MagPure Particles and Proteinase K, DNase I should be stored at 2–8°C upon arrival. However, short-term storage (up to 2 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified RNA can be used for clinical in vitro detection.