Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from bacteria, yeast cells |
| Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Bacteria, yeast cells |
| Sample amount | Bacteria: <10^9；Yeast cells：<2 x10^7 |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Kit Contents
| Contents | R418202 | R418203 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| Glass Beads (0.1-0.6mm) | 30 g | 150 g |
| DNase I | 600 μl | 5 x 600 μl |
| DNase Buffer | 6 ml | 30 ml |
| Protease Dissolve Buffer | 1.8 ml | 15 ml |
| Buffer ATL | 50 ml | 200 ml |
| Buffer PHC | 50 ml | 200 ml |
| Buffer GXP2* | 20 ml | 100 ml |
| Buffer RW1 | 50 ml | 250 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
Buffer PHC should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Buffer PHC up to 12 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components can be stored at roomtemperature (15–25°C) and are stable for at least 18 months under these conditions.
Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Name of Product
IL-6 / Interleukin-6 Control
Catalog Number
MQCO6 1
Short Info
This product is a Interleukin-6 Quality Control (two areas) for Milenia QuickLine IL-6 Test (MQL6 1).
This product is only available within the EU!
Method/Platform
control
Range/Assay Sensivity
two areas
Test Principle
The controls are designed only for internal Quality Control of Milenia QuickLine Il-6 Tests. Test results can only be evaluated with Milenia POCScan Reader.
Brief Instructions
Storage
2°C to 8°C
Components
IL-6 Control 1, IL-6 Control 2
2 x 3 flasks
This product is a Interleukin-6 Quality Control (two areas) for Milenia QuickLine IL-6 Test (MQL6 1).
Attogene’ s Cylindrospermopsin Lateral Flow Kit can be used to detect Cylindrospermopsin in source water samples.
Format: Rapid-Water – Run Time: 30 Minutes, enough to run two samples (undiluted and a 10 fold dilution) and negative control.
Cylindrospermopsin (CYN) is a potent cyanotoxin synthesized by select species of cyanobacteria, prominently including Cylindrospermopsin raciborskii. It belongs to the tricyclic alkaloid class, exhibiting a molecular weight of approximately 415 Da. Structurally, cylindrospermopsin features an uracil ring fused with a hydantoin moiety, alongside a guanidino group, attributes that render it highly soluble and polar in aqueous environments.
Cylindrospermopsin is notorious for its profound toxicity towards aquatic organisms and its potential threat to human health through exposure via contaminated water and food sources. Consequently, rigorous monitoring protocols are essential in regions prone to cyanobacterial blooms, where cylindrospermopsin can accumulate in freshwater reservoirs and other aquatic habitats. In recognition of these risks, regulatory bodies such as the United States Environmental Protection Agency (EPA) have implemented an action level guideline. As of 2019, EPA 10-day drinking water health advisory for cylindrospermopsin recommended a threshold of 0.7 parts per billion (ppb), or 700 parts per trillion (ppt) for children under the age of six, and 3 parts per billion, 3000 parts per trillion for anyone older, to effectively manage cylindrospermopsin levels. This precautionary measure aims to uphold both environmental sustainability and public health integrity by minimizing exposure risks. The EPA has also drafted a human health recreational water quality criterion to protect human health at 8,000ppt.
EPA 10-Day drinking water Health Advisories for Cylindrospermopsin:
Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions
Do not Drink – 3.0 μg/L for school age children to adults
Do Not Use – 20 μg/L
EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L
Screening of Cylindrospermopsin in water samples at or above 30ppt
Format: 15 tests (5 samples at 2 dilutions/5 controls)
5 – 10X Sample Dilution Buffer
5 – 1X Sample Dilution Buffer
5- Negative Control
10- Fixed Volume Pipettes