Description
Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.
Q-PAGE™ TGN Precast Gels are available in gradient (4 to 15%) and fixed (10%) concentrations of polyacrylamide in 12- and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP4XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP5XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels. Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Q-PAGE™ TGN (Tris-Glycine Novel) Precast Gels are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, Q-PAGE™ TGN Precast Gels perform enhanced speed, better separation, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in Q-PAGE™ TGN series, however, are similar with typical Laemmli Tris-HCl gels, and thus Q-PAGE™ TGN Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses.
endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereus. Purified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.
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| Kit Specifications (Spin Columns) | |
| Input | 2 x 109 bacterial cells |
| Column Binding Capacity | 25 µg |
| Average Yield* | Up to 20 µg |
| Time to Complete 10 Purifications | 1 hour |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
| Component | Cat. 17900 (50 preps) | Cat. 17950 (192 preps) |
|---|---|---|
| Resuspension Solution A | 20 mL | 60 mL |
| Lysis Buffer P | 18 mL | 60 mL |
| Solution BX | 28 mL | 110 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Buffer B | 30 mL | 2 x 30 mL |
| Proteinase K | 12 mg | 50 mg |
| Spin Columns | 50 | – |
| 96-Well Plate | – | 2 |
| Adhesive Tape | – | 4 |
| Collection Plate | – | 2 |
| Collection Tubes | 50 | – |
| Elution Tubes (1.7 mL) | 50 | – |
| Elution Plate | – | 2 |
| Product Insert | 1 | 1 |