Product Description
Unlocking The Potential Of Nucleic Acid Amplification With DNA Amplification Kit (Basic)
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit Basic |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(Basic type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation high pure total DNA from FFPE using high bind beads |
| Applications | PCR and viral DNA detection, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Paraffin embedded tissue samples |
| Sample amount | 1-6 slices of 10-20μm |
| Elution volume | ≥30μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
| Contents | D632301D | D632302D |
| Purification Times | 48 Preps | 96 Preps |
| MagPure Particles N | 1.1 ml | 2.5 ml |
| RNase A | 10 mg | 20 mg |
| Proteinase K | 24 mg | 48 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml |
| Buffer DPS | 60 ml | 100 ml |
| Buffer ATL | 15 ml | 30 ml |
| Buffer BST1 | 30 ml | 60 ml |
| Buffer BW1 | 13 ml | 44 ml |
| Elution Buffer | 15 ml | 30 ml |
Storage and Stability
Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
Cell Culture Plate 6 Wells
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells
12wells, 24wells., 48wells, 96wells