The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).
ChIP-Seq Library Prep Kit Workflow
ChIP-Seq is the combination of chromatin immunoprecipitation (ChIP) with next generation sequencing. It is a powerful tool for the analysis of global transcription factors and other proteins in diseases and biological pathways, and characterization of histone modifications in a genome-wide level at single-base resolution. ChIP-Seq delivers whole genome level of functional profiling of global transcription factors, and provides better understanding of epigenetic modifications.
Three index types are available for the ChIP-Seq Library Prep Kit of the illumina platform:
Non-index (Cat.# 30032): Libraries do not have index.
Index (Cat.# 30034): Each index primer contains a unique 6-base index sequence can be used for identification. 48 samples can be pooled together. Index information can be downloaded here.
Unique dual index (Cat.# 30036): The ChIP-Seq library multiplexing for 96 samples is possible. Our unique 4-Base Difference Index System have 8 bases index length and at least 4 bases are different from each other for better library identification. Our unique dual indexing primers remove sequencing errors such as index hopping, index contamination, mis-assignment, and other errors. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34034).
Kit advantages:
Comparison of library conversion efficiency under the same condition. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.
Comparison of aligned reads, aligned rate and duplication rate. Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used.
Data comparison: Input DNA amounts are 5 ng and 30 ng. BioDynami ChIP-Seq Library Prep Kit (Cat.# 30034) was used. Sequencing peak regions are shown.
The ChIP-Seq Library Prep Kit (illumina and MGI Platform) was developed for the construction of high quality ChIP-Seq libraries using 5 ng to 400 ng of ChIP DNA as input. The kit is compatible with ChIP DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.).
These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.
Plant/Fungi DNA Isolation Kit (Spin Column)
Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.
Plant/Fungi DNA Isolation Kit (Magnetic Bead System)
The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications – Spin Column | |
| Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Maximum Amount of Starting Material:Plant TissuesFungi (wet weight) | 100 mg 100 mg |
| Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight) | 18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.
Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits
| Plants | Plants (Cont’d) | Fungi |
| Tomato | Turnip | Aspergillus niger |
| Grape | Chinese Cabbage | Mucor racemosus |
| Peach | Radish | Cladosporium cladosporioides |
| Plum | Komatsuna | Fusarium oxysporum |
| Pine Needles | Apricot | Penicillium sp. |
| Raspberry | Sweet Potato | Botrytis cinerea (Botryotinia fuckeliana) |
| Strawberry | Hydrangea | Pichia sp. |
| Legumes | Fig | Rhizopus oryzae |
| Prosopis cineraria (Ghaf) | Turf | Alternaria tenuissima |
| Sorghum grass | Cherry | Fusarium sp. |
| Tobacco | Saintpaulia | |
| Arabidopsis | Lotus | |
| Lichen | Carrot | |
| Corn seed | Hansen | |
| Sunflower seed | Pistachio | |
| Olive seed | ||
| Soybean seed |
| Component | Cat. 26200 (50 preps) | Cat. 26250 (250 preps) | Cat. 26900 (192 preps) | Cat. 58200 (50 preps) | Cat. 62400 (192 preps) |
|---|---|---|---|---|---|
| Lysis Buffer L | 30 mL | 1 x 30 mL 2 x 60 mL | 2 x 60 mL | 60 mL | 2 x 60 mL |
| Binding Buffer I | 7 mL | 1 x 7 mL 1 x 25 mL | 25 mL | 7 mL | 25 mL |
| Solution WN | 18 mL | 1 x 18 mL 1 x 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 15 mL | 30 mL | 30 mL | 8 mL | 30 mL |
| RNAse A | 1 vial (80 μL) | 5 vials (5 x 80 μL) | 1 vial | 1 vial | 1 vial |
| Magnetic Bead Suspension | – | – | – | 4 x 1.1 mL | 2 x 8.5 mL |
| Filter Columns | 50 | 250 | – | – | – |
| Spin Columns | 50 | 250 | – | – | – |
| Collection Tubes | 100 | 500 | – | – | – |
| 96-Well Plate | – | – | 2 | – | 2 |
| 96-Well Collection Plate | – | – | 2 | – | – |
| Adhesive Tape | – | – | 4 | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 250 | – | 50 | – |
| 96-Well Elution Plate | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
This product provides high quality purification of total DNA from whole blood, plasma, serum, buffy coat, or other body fluids, lymphocytes and cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 60 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
This kit can use on manual protocol or 96 channel automated extraction system.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 200μl whole blood |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
| Sample amount | 200μl |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
| Contents | D631101 | D631102 | D631103 |
| Purification Times | 48 | 96 | 480 |
| MagPure Particles | 1.2 ml | 2.5 ml | 11 ml |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 1.8 ml | 5 ml | 15 ml |
| Buffer AL | 15 ml | 30 ml | 120 ml |
| Buffer GW1* | 22 ml | 53 ml | 220 ml |
| Elution Buffer | 15 ml | 30 ml | 100 ml |
Storage and Stability
Proteinase K, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
This product provides high quality purification of total DNA from whole blood, plasma, serum, buffy coat, or other body fluids, lymphocytes and cultured cells. There is no need to use toxic phenol chloroform extraction or time-consuming alcohol precipitation. The extraction process finish in 60 minutes. Purified DNA includes genomic DNA, mitochondrial DNA, viral DNA (e.g. HBV), or DNA from other parasitic microorganisms. The obtained DNA can be directly used in PCR, viral DNA detection and other experiments.
This kit can use on manual protocol or 96 channel automated extraction system.