This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Extract total pathogen nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant. |
| Applications | RT-PCR，PCR |
| Products | Pathogen DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant |
| Sample amount | 200-300μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Kit Contents
| Contents | IVD6672-50 | IVD6672 |
| Purification Times | 50 Preps | 200 Preps |
| Bead Tube C | 50 | 4 x 50 |
| MagPure Particle | 1.6 ml | 7.0 ml |
| Proteinase K | 24 mg | 100 mg |
| Protease Dissolve Buffer | 1.8 ml | 6 ml |
| Buffer MLBN | 30 ml | 120 ml |
| Buffer MW1* | 22 ml | 53 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Buffer NFW | 10 ml | 30 ml |
Storage and Stability
MagPure Particles and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
Norgen’s Saliva Exosome Collection and Preservation Kit is an all-in-one solution designed for 1) simple and non-invasive saliva collection; 2) preservation of exosomes in saliva samples at ambient temperature; and 3) isolation of high quality intact exosomes and exosomal RNA within a laboratory setting. The Saliva Exosome Collection, Preservation and Isolation Kit contains 50 Individual Saliva Exosome Collection and Preservation Devices. Each of the 50 individual Saliva Exosome Collection and Preservation Devices consists of 3 components:
Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed, the Collection Tube is securely capped with Collection Tube Cap. The collected saliva is mixed by gentle inversion for several times till the saliva colour changes into yellow. The Saliva Collection Tube is subsequently sent to the laboratory for the purification of intact exosomes and for the subsequent isolation and analysis of saliva exosomal RNA. The saliva exosomes in preserved samples are stable for 2 years at room temperature.
| Kit Specifications | |
| Volume of Saliva Collected | 2 mL |
| Preservation Temperature | Room Temperature |
| Preservation time | 2 years at room temperature |
Shelf Life and Handling
| Kit Components | Cat. 65400 (50 Devices) |
|---|---|
| Individual Saliva Exosome Collection and Preservation Devices | 50 |
| Donor Procedure Flowchart | 1 |
| Product Insert | 1 |
| Individual Saliva Exosome Collection and Preservation Device Contents | |
| Saliva Collection Funnel and Collection Tube | 1 |
| Collection Tube Cap | 1 |
| Donor Instructions | 1 |
Description
1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ TGN Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.
Features
Contents
0.025 M Tris, 0.192 M glycine, 0.10% SDS
Applications
Running buffer for Laemmli Tris-HCl gel electrophoresis
Storage
Room temperature for 24 months
1X Tris-Glycine-SDS Running Buffer Powder is used for electrophoresis on Q-PAGE™ TGN Precast Gel or Laemmli Tris-HCl gels in Tris-Glycine buffer system. It is convenient and universal for electrophoresis in Tris-Glycine buffer system.