K-RHAMNOSE

SKU: 700004334

50 / 100 assays (manual) / 550 assays (microplate) / 550 assays (auto-analyser)

Content:	50 / 100 assays (manual) / 550 assays (microplate) / 550 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 1 year under recommended storage conditions

Analyte:	L-Rhamnose
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	5 to 100 µg of L-rhamnose per assay
Limit of Detection:	~ 1.2 mg/L
Reaction Time (min):	~ 5 min at 25oC or ~ 4 min at 37oC
Application examples:	Hydrolysates of plant material and polysaccharides, culture media / supernatants and other materials.
Method recognition:	Novel method

The L-Rhamnose Assay Kit for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials is a simple, rapid method.

L-Rhamnose occurs naturally in the L-form and is commonly present as a component of the carbohydrate moiety of eukaryotic glycoproteins and in plant cell wall polysaccharides. The most abundant occurrence of L-rhamnose is within the pectic fraction of plant cell wall polysaccharides. L-Rhamnose is commonly used as a non-metabolisable marker along with lactulose for dual-permeability testing in the diagnosis of intestinal diseases such as Crohn’s disease or coeliac disease.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View more of our monosaccharide and disaccharide assay kits.

Advantages

• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Only test kit available 
• Simple format 
• Rapid reaction (~ 5 min at 25^oC or ~ 4 min at 37^oC) 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The L-Rhamnose Assay Kit for the measurement of L-rhamnose in plant extracts, culture media/supernatants and other materials is a simple, rapid method.

Introduction

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA and miRNA from cell and tissue without MagZol reagent
Applications	RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Cell, Animal tissue, Plant tissue
Sample amount	Cells: ≤ 5 x 10^6, Animal tissue:<10mg
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.

Advantages

• High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast – several samples can be extracted in 40 minutes
• High applicability – samples including animals, plants, bacteria, cells, etc.
• High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
• Safe – no phenol chloroform extraction and no use of Trizol reagent

Kit Contents

Contents	R431102	R431103
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	100	2 x 250
2ml Collection Tubes	100	2 x 250
Proteinase K	48 mg	240 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer RLC	40 ml	200 ml
Buffer RWC	20 ml	80 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

Introduction

This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from tissue, cell
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor, pipetting workstation
Sample type	Tissues, cells, lymphocytes and other clinical sample
Sample amount	Cells grown in suspension:3~5 x 106Animal tissue: 10~20mgPlant tissue: ≤100mg

Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water

Advantages

• High purity – OD260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – extraction without manual participation, saving time and effort
• Universal – suitable for various clinical samples

Kit Contents

Contents	IVD3020
Purification Times	200 Preps
MagPure RNA Particles	7 ml
DNase I	4 x 600 µl
DNase Buffer	80 ml
RTL Lysis Buffer	150 ml
Buffer MCB*	75 ml
Buffer MW1*	110 ml
Buffer MW2*	50 ml
RNase Free Water	60 ml

Cat.No	Reagent	IVD3020-F-96
DNase Buffer		60 ml
DNase I		2 x 600 μl
RTL Lysis Buffer		80 ml
Buffer MCB		18 ml
96-Tip		1
Sample plate (DW Plate)	500μl Buffer MCB	1
Wash 1 Plate (DW Plate)	700μl Buffer MW130μl MagPure RNA Partilces	1
DNase Plate	Empty
Wash 2 Plate (DW Plate)	700μl Buffer MW1	1
Wash 3 Plate (DW Plate)	900μl Buffer MW2	1
Elution plate (DW Plate)	80μl RNase Free Water	1

Cat.No	Reagent	IVD3020-TL-06
Purification times		96 Preps
DNase I		2 x 600 μl
DNase Buffer		60 ml
RTL Lysis Buffer		60 ml
Buffer MCB		40 ml
96-Tip		12 PCS
2.0ml V-bottom plate	Row 1/7：500μl Buffer MCBRow 2/8：500μl Buffer MW1Row 3/9：emptyRow 4/10：30μl Magpure RNA Particles500μl Buffer MW2 Row 5/11：900μl Buffer MW2 Row 6/12：80μl RNase Free Water	6

Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.