12x10ml PRP Centrifuge Rotor

K-LOLAC

SKU: 700004314

65 assays per kit

Content:	65 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Lactose
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose)
Limit of Detection:	1.62 mg/L
Reaction Time (min):	~ 10 min
Method recognition:	AOAC Method 2020.08

The K-LOLAC test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products. This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.

We offer more monosaccharide and oligosaccharide assay kit products.

View Lactose Assay Kit (K-LOLAC) Flyer

View Product Poster Presentation – Single Lab Validation for K-LOLAC.

Validation of Methods

Advantages

• World’s first sequential assay for lactose, i.e. improves accuracy and efficiency 
• Contains a specific β-galactosidase for the selective measurement of lactose in dairy products 
• Efficient pre-treatment step allows for accurate measurement of lactose in “low-lactose” and “lactose-free” dairy products 
• Lower limit of detection (LOD) than any other commercially available enzymatic lactose detection method. LOD at 1.62 mg/L 
• Very competitive price (cost per test)

Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

K-ACET

SKU: 700004254

53 assays per kit

Content:	53 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Acetic Acid
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	0.3 to 20 µg of acetic acid per assay
Limit of Detection:	0.14 mg/L
Reaction Time (min):	~ 14 min
Application examples:	Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by EN, ISO,ICUMSA, IFU and MEBAK

The Acetic Acid (ACS Manual Format) test kit is a simple method for the rapid and reliable measurement and analysis of acetic acid/acetate in foods, beverages and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

See our full range of organic acid test kits.

Advantages

• No wasted ACS solution (stable suspension supplied) 
• PVP incorporated to prevent tannin inhibition 
• All reagents stable for > 2 years after preparation
• Very competitive price (cost per test) 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing

The Acetic Acid (ACS Manual Format) test kit is a simple method for the rapid and reliable measurement and analysis of acetic acid/acetate in foods, beverages and other materials.