Description
The DM3200 ExcelBand™ 1 KB Plus (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM3200 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 10,000 bp
Concentration
87 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months
4°C for 12 months
-20°C for 36 months
The DM3200 ExcelBand™ 1 KB Plus (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder DM3200 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
| TGL20 Technical Parameters | |||
| Max Speed | 21000r/min | Max Volume | 6×100ml |
| Timer | 1~99h59min | Dimension | 570×620×380mm |
| Speed Accuracy | ±20r/min | Max RCF | 30910×g |
| Noise | ≤58dBA | Net Weight | 84KG |
| Power supply | AC 220V, 50HZ 10A | Temperature Range | -20℃~40℃ |
| Temperature Accuracy | ±1℃ | ||
Features:
1. Digital display indicates the speed,time,temperature and RCF.
2. Brushless DC motor in great torque, free maintenance,no powder pollution.quick in speed up and down.
3. There are many kinds of rotors for choice.
4. Automatically electric lid lock,super speed,over temperature protection and imbalance protection.
5. The centrifuge body is made of high-quality steel,safe and reliable.
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 100-150mg stool samples |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Stool |
| Sample amount | 100-150 mg |
| Elution volume | |
| Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
| Contents | D636401 | D636402 | D636404 |
| Purification Times | 48 Preps | 96 Preps | 400 Preps |
| MagPure Particles N | 1.7 ml | 3.4 ml | 14 ml |
| 2ml Bead Tubes | 48 | 96 | 400 |
| RNase A | 10 mg | 20 mg | 75 mg |
| Proteinase K | 24 mg | 48 mg | 180 mg |
| Protease Dissolve Buffer | 3 ml | 5 ml | 20 ml |
| Buffer ATL | 60 ml | 110 ml | 300 ml |
| PVP-10 | 1.2 g | 2.2 g | 6 g |
| Buffer PCI | 50 ml | 100 ml | 300 ml |
| Buffer MLE | 30 ml | 60 ml | 180 ml |
| Buffer GW1* | 22 ml | 44 ml | 132 ml |
| Elution Buffer | 20 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.