12×10ml Swing Buckets Rotor

Description

Specifications

Transcription Factor E3 (TFE3) is a transcription factor that binds to the MUE3-type E-box sequences involved in TGF-β signalling. Anti-TFE3 staining is the most sensitive and specific indicator of Xp11 translocation renal cell carcinomas. Since alveolar soft part sarcoma (ASPS) is characterized by a specific chromosomal rearrangement resulting in a chimeric transcription factor (ASPSCR1-TFE3), this TFE3 IVD antibody is also a useful diagnostic tool for recognizing ASPS.

Introduction

Usages:

For the rapid detection and enumeration of Escherichia coli.

Principle:

Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine coli β- glucuronidase enzyme specific reaction, hydrolysis of the substrate, the release of the color groups produce green colonies on the light yellow plate.

Formulation (per liter):

Peptone :15.0g

Yeast extract powder: 3.0g

Sodium chloride: 5.0g

Sodium lauryl sulfate:0.1g

Agar: 12.0g

Chromogenic substrate 6.5g

Final pH 7.0 ± 0.2

How to use:

1. Weigh 41.6g of the product, adding 1.0L distilled or deionized water, heated to boiling stirring until completely dissolved, dispensing into flask, 115 autoclaved for 10minutes.

2. Take 25.0g or 25.0ml of sample with sterile procedures , added  to the flask containing 225.0mL of sterile phosphate buffered saline (or saline) , shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 medium.

3, observe the results.

Quality Control

This product appears light yellow after the pouring on plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.

Bacteria name            Bacteria NO.       Growth Situation         Feature

Escherichia coli          ATCC25922          good            green colonies

Citrobacter              ATCC8090           good           colorless colonies

Salmonella typhimurium   CMCC50115         good            colorless colonies

Enterococcus faecalis     ATCC29212         suppressed                —–

Storage: Store in a dark, cool and dry place, tighten the caps immediately after use. Storage period of two years.

1000mL

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings