Norgen’s Milk DNA Preservation and Isolation Kit is an all-in-one solution designed for 1) preservation of DNA in milk samples at ambient temperature; and 2) isolation of high quality DNA within a laboratory setting. The kit is provided with a Preservation Solution that allows for either immediate processing of milk samples, or allows for the storage of the milk samples for up to 1 month at room temperature prior to DNA isolation. Genomic DNA extracted from somatic cells and bacteria found in milk can be used in various applications. The isolated DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Milk DNA can also be used to diagnose particular types of infections. Milk DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Milk DNA Preservative
Norgen’s Milk DNA Preservation Solution is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the preservative allow samples to be stored for 1 month without any detectable DNA degradation.
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| Kit Specifications | |
| Maximum Milk Input | 0.5 mL |
| Time to Complete 10 Purifications | 80 minutes (20 minutes hands on) |
Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Storage in Preservation Buffer
1) Storage at -20°C is recommended for archival samples and will provide optimal preservation. The preservation buffer will freeze at -20°C. Samples can be stored indefinitely at -70°C.
2) Samples can be stored at room temperature (25°C) for up to 1 month without significant loss of DNA quality.
3) DNA has also been successfully isolated from samples stored at 37°C 1 for one week.
| Kit Components | Cat. 44800 (25 preps) |
|---|---|
| Preservation Solution E | 30 mL |
| Proteinase K | 0.6 mL |
| Purification Additive | 0.5 mL |
| Buffer SK | 40 mL |
| Wash Solution A | 18 mL |
| Elution Buffer B | 8 mL |
| Mini Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
Lactulose Assay Kit
| Content: | 50 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Lactulose |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.65 to 65 µg of lactulose per “Enzymatic Determination Reaction |
| Limit of Detection: | 4.8 mg/L |
| Reaction Time (min): | ~ 120 min |
| Application examples: | Milk, dairy products and foods containing milk. |
| Method recognition: | Novel method |
The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.
Check out our other monosaccharide and disaccharide assay kits.
Advantages
The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.
Propargyl-PEG5-alcohol enables Click Chemistry reactions with azide compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG5-alcohol enables Click Chemistry reactions with azide compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.