Description
The DM3160 FluoroBand™ 1KB (0.25-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA ladder DM3160 is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
250 ~ 10,000 bp
Concentration
50 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light
Room temperature for 6 months
4°C for 12 months
-20°C for 24 months
The DM3160 FluoroBand™ 1KB (0.25-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA ladder DM3160 is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
K-FRGLQR
SKU: 700004283
1100 assays (microplate) / 1100 assays (auto-analyser)
| Content: | 1100 assays (microplate) / 1100 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 6 months under recommended storage conditions |
| Analyte: | D-Fructose, D-Glucose |
| Assay Format: | Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.4 to 20 µg of D-glucose plus D-fructose per assay |
| Limit of Detection: | 133 mg/L |
| Reaction Time (min): | ~ 13 min |
| Application examples: | Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC |
This product has been discontinued, you may be interested in the following replacement product K-FGLQR; 700007621)
The D-Fructose/D-Glucose (Liquid Ready) test kit is a rapid, reliable and accurate method for the specific measurement and analysis of D-fructose and D-glucose in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for auto-analyser and microplate formats.
Find more of our monosaccharide and oligosaccharide assay kits.
Advantages
This product has been discontinued, you may be interested in the following replacement product K-FGLQR; 700007621)
N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Propargyl-PEG4-carbonyl)-N-bis(PEG1-methyl ester) is a crosslinker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.