Clone | IHC005 |
Source | Mouse Monoclonal |
Positive Control | Tonsil |
Dilution Range | 1:200 |
PAX-5 is a member of the paired box (PAX) family of transcription factors, which are key regulators in early development. The PAX-5 gene encodes the B-cell lineage specific activator protein (BSAP), whose expression is limited to early stages of B-cell differentiation. Anti-PAX-5 is useful in differentiating between classic Hodgkin’s lymphoma versus multiple myeloma and solitary plasmacytoma, as the protein is expressed in mature and precursor B-cell non-Hodgkin’s lymphomas/leukemias while being absent from the other two conditions. Diffuse large B-cell lymphomas are positive for PAX-5, with the exception of those with terminal B-cell differentiation, and T-cell neoplasms do not stain with Anti-PAX-5.
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
Specifications
Features | Specifications |
Main Functions | Isolation high pure total DNA from FFPE using high bind beads |
Applications | PCR and viral DNA detection, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Paraffin embedded tissue samples |
Sample amount | 1-6 slices of 10-20μm |
Elution volume | ≥30μl |
Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Contents | D632301D | D632302D |
Purification Times | 48 Preps | 96 Preps |
MagPure Particles N | 1.1 ml | 2.5 ml |
RNase A | 10 mg | 20 mg |
Proteinase K | 24 mg | 48 mg |
Protease Dissolve Buffer | 3 ml | 6 ml |
Buffer DPS | 60 ml | 100 ml |
Buffer ATL | 15 ml | 30 ml |
Buffer BST1 | 30 ml | 60 ml |
Buffer BW1 | 13 ml | 44 ml |
Elution Buffer | 15 ml | 30 ml |
Storage and Stability
Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 100-150mg stool samples |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Stool |
Sample amount | 100-150 mg |
Elution volume | |
Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Kit Contents
Contents | D6364 |
Purification Times | 400 Preps |
MagPure Particles N | 14 ml |
2ml Bead Tubes | 400 |
PVP-10 | 6 g |
RNase A | 75 mg |
Proteinase K | 180 mg |
Protease Dissolve Buffer | 20 ml |
Buffer ATL | 300 ml |
Buffer PCI | 300 ml |
Buffer MLE | 180 ml |
Buffer GW1* | 132 ml |
Elution Buffer | 60 ml |
Storage and Stability
MagPure Particles, RNase A and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
MagPure Stool DNA Kit is specially designed for high throughput DNA extraction from stool samples. It can get high purity microbial DNA from stool samples (≤200mg). This kit is based on magnetic beads purification and unique inhibiting factors adsorption technology, no phenol-chloroform extraction or alcohol precipitation. It can adsorb humic acid and other inhibiting factors in the solution efficiently. DNA can be directly used for downstream applications such as PCR, Viral DNA testing, bacterial DNA testing, etc.
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