Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
N-(Propargyl-PEG2)-N-Boc-PEG3-t-butyl ester is a click chemistry branched linker. The propargyl group can react with azide-bearing compounds through Click Chemistry, the t-butyl protected carboxyl group can also be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Propargyl-PEG2)-N-Boc-PEG3-t-butyl ester is a click chemistry branched linker. The propargyl group can react with azide-bearing compounds through Click Chemistry, the t-butyl protected carboxyl group can also be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format. Trypsin is added to the protein sample and bound to the column. Salts are washed away and the trypsin is then activated to digest proteins. Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry. Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation). The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more.
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| Kit Specifications | |
| Maximum Protein Input | 15 μg |
| Minimum Protein Input | 2 μg |
| Minimum Elution Volume | 30 μL |
| Time to Process 10 Purifications | 20 minutes (Plus a 1-hour incubation) |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 17500 (25 preps) |
|---|---|
| Wash Solution C | 30 mL |
| Binding Buffer A | 4 mL |
| Column Activation Buffer | 3 mL |
| Micro Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution tubes (1.7 mL) | 25 |
| Product Insert | 1 |