12×20ml Angle Rotor 

The Opentrons 24-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block acts as a 24 position rack to keep 2mL tubes, 1.5mL tubes, and the NEST 0.5mL tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).

The Opentrons 24-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block acts as a 24 position rack to keep 2mL tubes, 1.5mL tubes, and the NEST 0.5mL tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).

Description

The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl₂, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.

Features

• 5’→3’ DNA polymerase activity
• No detectable 3’→5′ exonuclease (proofreading) activity
• Generates PCR products with 3′-dA overhangs
• High yield PCR 
• High reproducibility
• Reduced  pipetting errors
• Includes tracking dye for direct loading after PCR

Applications 

• Routine PCR
• Colony PCR
• High throughput PCR
• Amplification of DNA fragments up to 8 kb
• Generation of PCR products for TA cloning
• DNA labeling

Storage

4°C for 6 months
-20°C for 24 months

The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.

• All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
  + Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback). 
  + Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
  + Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.   
  + Lyo ready. Contains all necessary excipients needed for freeze-drying. Can be used for lyophilization. Enables RT storage and shipping once dried. Can be freeze-dried by us. Learn MoreFor research use and further manufacturing. Designed and manufactured under ISO13485.

Exemplary tetraplex PCR assay

Detection of three specific pathogen targets and an internal control. Total input DNA per reaction was 10^6 (red), 10^5 (yellow), 10^4 (blue), 10^3 (green), 10^2 (purple), 10 (light blue) and 0 copies (grey) and 8000 copies/reaction for the internal control (shown in the CY5 plot).

5x Multiplex – robust PCR perforemance for a wide range of qPCR application

PlexTaq 5x qPCR Multiplex Master Mix contains all components necessary for rapid, sensitive and reproducible quantification of DNA and cDNA. An engineered DNA polymerase and an optimized buffer including ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents aptamer prevents false amplification and provides a fast start function. 

PlexTaq®´s formulation allows to use it also for direct PCRs from crude samples.

All in One. All the flexibility you need in a 5x Master Mix for multiplexing applications. NOW LYO READY!

+ Sensitive. More free volume. 5x concentration allows more volume for target specific primers and probes (multiplexing).
+ Robust. Uniform amplification. Up to 30 target multiplexing in real-time (customer feedback).
+ Fast TTR. No extraction needed. Reliable results with crude samples without extraction step, like blood.
+ Specific. No false amplification. Engineered Taq DNA polymerase more stable at room temperature. Aptamer-based hot-start prevents false amplification and provides a fast-start function.