12×50ml Angle Rotor 

Name of Product

Echinococcus multilocularis (Em2-Em18) – IgG ELISA

Catalog Number

AF 9300

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus multilocularis in human Serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample bind to Echinococcus multilocularis Em2-Em18 antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.

Brief Instructions

Storage

2-8°C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

12 x 8 strips (96 tests)

This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus multilocularis in human Serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Overview

• Compatible with PAXgene Blood RNA Tubes
• Isolate true total RNA including siRNA and microRNA
• No phenol extractions
• Purify high-quality RNA in 30 minutes
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Preserved Blood RNA Purification Kit II provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.

* PAXgene Blood RNA Tubes are a product of PreAnalytiX GmbH.  PAXgene Blood RNA Tubes are not provided.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	30 minutes
Average Yield	5 – 20 μg per 2.5 mL preserved human blood

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The DNase I should be stored at -20°C.

Component	Cat. 43500 (50 preps)
NPX1	2 x 110
NPX2	40 mL
NPX3	22 mL
NPX4	6 mL
NPX5	6 mL
DNase I	1 vial
Mini Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Overview

• Isolate all sizes of circulating and exosomal RNA, including microRNA
• Versatile plasma and serum input volumes
• Concentrate circulating and exosomal RNA into small elution volumes
• Isolate inhibitor-free circulating and exosomal RNA
• Bind and elute all RNA irrespective of size or GC content, without bias
• Available in Spin Column or 96-well plate formats
• Process 96-well plates using vacuum or centrifugation
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Compatible with Streck Cell-Free RNA BCT® Tubes
• Purification is based on Norgen’s proprietary resin matrix

These kits are able to isolate all sizes of circulating and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR. The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.

Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL.

Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Mini Slurry)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL.

Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (High Throughput Slurry)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating circulating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation.

Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Maxi Slurry)

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from various amounts of plasma/serum ranging from 2 mL to 5 mL.

Details

Supporting Data

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Kit Specifications – 96-well
Minimum Plasma/Serum Input	0.25 mL
Maximum Plasma/Serum Input	2 mL
Size of RNA Purified	All sizes, including microRNA
Time to Complete Purification	< 1 hour

Cat.29500 Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 51000 (50 preps)	Cat. 29500 (96 preps)	Cat. 42800 (50 preps)	Cat. 50900 (25 preps)
Slurry C1	–	–	–	6 mL
Slurry C2	12 mL	–	12 mL	–
Slurry C3	–	20 mL	–	–
Lysis Buffer A	2 x 130 mL	4 x 100 mL	2 x 130 mL	2 x 130 mL
Wash Solution A	38 mL	2 x 38 mL	38 mL	18 mL
Elution Solution A	6 mL	20 mL	6 mL	6 mL
Mini Filter Spin Columns	50	–	50	25
96-Well Filter Plate	–	1	–	–
Adhesive Tape	–	1	–	–
Collection Tubes	50	–	50	25
96-Well Collection Plate	–	1	–	–
Elution Tubes (1.7 mL)	50	–	50	25
96-Well Elution Plate	–	1	–	–
Product Insert	1	1	1	1