029070 Nitrate Reduction Reagent
Usage:For Nitrate Reduction Test.
Specification:10ml*2vials
10ml*2vials
Clone | IHC506 |
Source/Clonality | Mouse Monoclonal |
Positive Control | Appendix, Uterus, Vessel Wall |
Dilution Range | 1:200 |
Actin is part of the cytoskeletal system of all cell types. Smooth Muscle Actin is found in myofibroblasts and myoepithelium, but not in cardiac and skeletal muscles. Labelling of smooth muscle actin in concert with muscle specific actin staining can allow for differentiation between rhabdomyosarcoma and leiomyosarcoma, as Muscle specific actin is found in rhabdomyoblasts, while smooth muscle actin is found in leiomyosarcomas.
This kit is designed for the rapid preparation and purification of DNA fragments that have been fractionated on agarose gels. The recovered DNA is free from agarose and other impurities, and is compatible with restriction enzyme digestion, ligation into vectors and sequencing. The protocol can be completed in 20 minutes.
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Kit Specifications | |
Column Binding Capacity | 10 μg |
Maximum Weight of Gel Slice | 400 mg |
Average Recovery | 70-90% |
Size of DNA Purified | 100 – 15,000 bp |
Minimum Elution Volume | 30 μL |
Time to Complete 10 Purifications | 30 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component | Cat. 13100 (50 preps) |
---|---|
Binding Buffer G | 80 mL |
Wash Solution A | 12 mL |
Elution Buffer B | 8 mL |
Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
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