Overview

• Reliable and cost-effective
• Non-invasive, user-friendly sample collection and preservation in one convenient kit
• Preserved RNA is stable for up to 2 months at room temperature
• Compatible with most RNA isolation methods including Norgen’s Saliva/Swab RNA Purification Kits
• High quality RNA is suitable for sensitive downstream applications including RT-PCR, RT-qPCR, RNA sequencing and microarray
• Inactivates viruses including SARS-CoV-2 upon mixing – Explore the Application Note
• Shipping accessories can be purchased separately
• CE-IVD marked version available

Norgen’s Saliva RNA Collection and Preservation Devices are designed for simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices consists of 3 components:

• Saliva Collection Funnel and Collection Tube
• Collection Tube Cap
• Norgen’s Saliva RNA Preservative contained within a sealed squeezable ampoule

Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The Saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for epidemiological and population studies.

Saliva RNA Preservative

Norgen’s Saliva RNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of RNA from fresh specimens. The buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for up to 2 months at room temperature.

Nucleic Acid Isolation from Preservative

Saliva RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300).

Details

Supporting Data

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Kit Specifications
Volume of Saliva Collected	2 mL
Volume of Saliva-Preservative Mix	4 mL
Preservation Temperature	Room Temperature
Preservation time	Up to 2 months at room temperature

Shelf Life and Handling

• When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
• Once collected, saliva RNA is stable for up to 2 months when kept tightly sealed and stored at room temperature.
• The Collection Tube, the Collection Funnel and the Device Container of each individual Saliva RNA Collection and Preservation Device are recyclable.

Kit Components	Cat. RU53800 (50 Devices)
Individual Saliva RNA Collection and Preservation Devices	50
Donor Procedure Flowchart	1
Product Insert	1
Individual Saliva RNA Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube	1
Collection Tube Cap	1
Preservative Ampoule	1
Donor Instructions	1

Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.2g), purification of DNA from PCR or enzymatic reaction solution solution
Applications	PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method	Micro spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Agarose gel, PCR products, enzyme products
Sample amount	Agarose gel: ≤200mgDNA products: ≤300µl (≤5µg)
Recovery	100-5000bp：85%, 5-10kb：60-70%
Elution volume	≥10μl
Time per run	≤30 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	20µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – up to 80% DNA recovery
• Low elution volume – elution volume can be as low as 10μl
• General – recover DNA fromgel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method

Kit Contents

Contents	D211002	D211003
Purification Times	100 Preps	250 Preps
Buffer GDP	60 ml	125 ml
Buffer DW1	40 ml	90 ml
Buffer DW2	20 ml	50 ml
Elution Buffer	20 ml	30 ml
HiPure DNA Micro Columns	100	250
2 ml Collection Tubes	100	250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.

Description

Blood Urea Nitrogen Enzymatic Kit is a microplate-based colorimetric
assay for the determination of urea in serum samples produced from blood. Blood urea nitrogen (BUN) is an important marker for normal kidney and liver function. Elevation of BUN levels is often an indication of intestinal and kidney obstruction and cardiac failure. Decreased BUN levels are often associated with kidney and liver damage. BUN is also a very useful tool
for preclinical investigation of experimental drug formulations and BUN levels are commonly used to monitor and attenuate the toxic effects of experimental drug formulations in rodents.

Blood Urea Nitrogen Enzymatic Kit uses an enzyme-based assay to determine urea in liquid samples such as serum. The test is based on a highly proven method for urea determination. The Blood Urea Nitrogen Enzymatic Kit contains sufficient materials to test 42 samples in duplicate. The assay utilizes urease, a metabolic enzyme, to specifically detect urea in serum. The Blood Urea Nitrogen Enzymatic Kit provides rapid, accurate, proven results even in complex liquid mixtures. The limit of detection for the test is 8 ppm urea for serum. The linear range of the assay is 8 – 200 ppm analyte.

96 determinations
Rapid and simple method
Minimal sample prep
Highly accurate and reproducible