This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples |
Applications | Second generation sequencing, PCR, real timePCR, etc. |
Purification method | Monodisperse magnetic beads |
Purification technology | Magnetic beads technology |
Process method | Manual or automatic |
Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
Sample amount | Body fluid : 10 – 200μl, Tissue : ≤10mg |
Yield | 10ng – 15μg |
Elution volume | 80 -100μl |
Time per run |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Contents | IVD3101 |
Purification Times | 200 |
MagBind Particles | 4.5 ml |
Proteinase K | 90 mg |
Protease Dissolve Buffer | 10 ml |
Buffer ATL | 60 ml |
Buffer AL | 60 ml |
Buffer BD* | 20 ml |
Buffer BXW1* | 110 ml |
Elution Buffer | 30 ml |
Storage and Stability
Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
As this is a 2 gene kit, we recommend purchase of 2 of the accompanying RT-qPCR master mix reagent: oasig Lyophilised OneStep RT-qPCR Master Mix 150 reactions.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Norgen’s Cells and Tissue DNA Isolation Kits are designed for the rapid preparation of genomic DNA from cultured cells as well as various tissue samples and urine. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with PCR and Southern Blot analysis.
Cells and Tissue DNA Isolation Kit (Spin Column)
Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The protocol can be completed in approximately 30 minutes for cells and within 90 minutes for tissues. Each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Micro Kit (Micro)
Optimized for small inputs of cells and tissues, such as Laser-Captured Microdissection (LCM). Purification is based on spin column chromatography as the separation matrix. Norgen’s columns bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. Preparation time for a single sample is approximately 60 minutes, and each kit contains sufficient materials for 50 preparations.
Cells and Tissue DNA Isolation Kit (Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. Preparation for 10 purifications is approximately 40 minutes of hands-on time.
Cells and Tissue DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
Purification is based on the use of magnetic beads that bind DNA under optimized binding conditions. Norgen’s Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) allows for the isolation of genomic DNA from various types of animal tissues or cell samples. The Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) also can be integrated with a robotic automation system.
Figure 1 / 9
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Kit Specifications | |
Maximum Cells and Tissue Input | 20 mg of animal tissue 3 x 106 cells |
Average Yield* | 8-10 µg (20 mg of animal tissue) 8-12 µg (3 x 106 cells) |
Average Purity (OD260/280) | 1.8 – 1.9 |
Time to Complete 10 Purifications | 40 minutes hands-on time (Cat. 59100) 50 minutes hands on time (Cat. 62500) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). This kit is stable for 1 year after the date of shipment.
Component | Cat. 53100 (50 preps) | Cat. 57300 (50 preps) | Cat. 59100 (50 preps) | Cat. 62500 (192 preps) |
---|---|---|---|---|
Lysis Buffer B | 20 mL | 20 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
Solution WN | 18 mL | 18 mL | 18 mL | 55 mL |
Wash Solution A | 18 mL | 18 mL | – | – |
Elution Buffer B | 30 mL | 8 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
Proteinase K | 1.2 mL | 1.2 mL | 1.2 mL | – |
Proteinase K in Sotrage Buffer | – | – | – | 4 mL |
Magnetic Bead Suspension | – | – | 2 x 1.1 mL | 8.5 mL |
96-Well Plate | – | – | – | 2 |
Spin Columns | 50 | – | – | – |
Micro Spin Columns | – | 50 | – | – |
Collection Tubes | 50 | 50 | – | – |
Elution Tubes (1.7 mL) | 50 | 50 | 50 | – |
96-Well Elution Plate | – | – | – | 2 |
Adhesive Tape | – | – | – | 2 |
Product Insert | 1 | 1 | 1 | 1 |
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Tel : 081-875-1869 , 02-328-7179
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