12x7ml Angle Rotor 

Checkit Go enables you to easily measure the volumes dispensed by a robot or multi-channel pipette, in under 10 seconds. These models measure up to 8 channels at a time and are offered in volume ranges covering 2μL to 50μL.

Please choose the model that matches your volume range:

Model	Volume Range
5 μL Volumetric Verification Kit	2-5 μL
10 μL Volumetric Verification Kit	4-10 μL
20 μL Volumetric Verification Kit	8-20 μL
50 μL Volumetric Verification Kit	20-50 μL

Checkit Go enables you to easily measure the volumes dispensed by a robot or multi-channel pipette, in under 10 seconds. These models measure up to 8 channels at a time and are offered in volume ranges covering 2μL to 50μL.

Please choose the model that matches your volume range:

Model Volume Range
5 μL Volumetric Verification Kit 2-5 μL
10 μL Volumetric Verification Kit 4-10 μL
20 μL Volumetric Verification Kit 8-20 μL
50 μL Volumetric Verification Kit 20-50 μL

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• 10 discrete bands from 50 bp to 500 bp
• Higher intensity reference band at 250 bp

The 50 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains ten discrete fragments ranging from 50 bp to 500 bp with a higher intensity reference band at 250 bp. This Ladder is ideal for quick sizing of PCR products

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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50 bp DNA Ladder (Cat# 13525) – 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 50 bp to 500 bp
• Higher intensity band at 250 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	500	79
2	450	67
3	400	59
4	350	55
5	300	49
6	250	76
7	200	33
8	150	22
9	100	31
10	50	29

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Description 

The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease. 

Composition 

• 0.03% Xylene cyanol FF 
• 0.03% Bromophenol blue
• 0.15% Orange G 
• 10 mM Tris-HCl (pH 8.0) 
• 60% glycerol 
• 60 mM EDTA

Storage 

4°C for 12 months
-20°C for 36 months

The ExcelDye™ 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.