Permagen’s 15 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 15 mL Centrifuge tubes
Detail
Permagen’s 15 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 15 mL Centrifuge tubes
Accommodates any common 15 mL Centrifuge Tubes
Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
Apply to drying in laboratories such as cosmetics, fermentation broth, soil, materials, etc.
product description:
9kg-12kgs water capture capacity, which can meet the needs of laboratories with large freeze-drying requirements for water capture capacity
-70°C/-90°C ultra-low temperature cold trap, which can capture lower temperature organic solvents
Unique refrigeration technology, the cooling time of the cold trap from the start of the low-temperature refrigeration system to the target value is ≤3 minutes
Hemispherical cold trap bottom surface is more conducive to the discharge of condensed water
The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry a large amount of ultra-low temperature organic solvents
One-click hot gas defrost, with defrost compressor protection program.
Large flat flange silicone seal, permanently no need to apply any auxiliary sealing silicone grease
High-precision ultra-wide width detection, vacuum sensor with full-range automatic temperature compensation, making vacuum display more accurate
The cavity vacuum can be read and controlled accurately, and the vacuum control accuracy is ≤0.1mbar
Removable sample shelf with dedicated holes for centrifuge tube placement, making placement and height adjustment easier
Configuration of various types of chambers and T-frames can fully satisfy users’ sample placement
Optional configuration of eutectic point detection system and data export system.
The vacuum pump and sample placement chamber can be combined at will according to user needs, making the configuration more flexible and convenient
Techinical parameter
9-12L Floor tabletop and floor standing one for lab usage
Tabletop
BD1-9
BD2-9
BD1-12
BD2-12
Floor standing
BH1-9
BH2-9
BH1-12
BH2-12
Water Capture capacity
9L
12L
Cold trap temperature
-70 C
-90℃
-70C°
-90℃
Cold trap volume
15L
20L
Freeze dried chamber
High permeability organic glass/multi manifold external valve/stainless steel chamber
T4 DNA Ligase is an ATP and Mg2+ dependent dsDNA ligase which catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids. T4 DNA Ligase is active on both blunt-end and cohesive-end substrates. It is also completely inactivated by incubating at 70°C for 10 minutes.
This is a high-quality (commercial grade) version of the T4 DNA Ligase. T4 DNA Ligase is recombinantly produced in E. coli. ArcticZymes’ T4 DNA Ligase is extensively tested for contaminating DNase and RNase activities as well as residual host-cell gDNA.
Key Features
ATP and Mg2+ dependent dsDNA ligase
Easily heat-inactivated at 70°C for 10 minutes
Extensively tested for contaminating DNase and RNase activities as well as residual host-cell gDNA
Applications
Ligation of dsDNA
NGS library prep
Molecular cloning
Figures
Document
T4 DNA Ligase is an ATP and Mg2+ dependent dsDNA ligase which catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids. T4 DNA Ligase is active on both blunt-end and cohesive-end substrates. It is also completely inactivated by incubating at 70°C for 10 minutes.
Ten discrete fragments ranging from 300 bp to 5000 bp
The Norgen MidRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our MidRanger contains ten discrete fragments ranging from 300 bp to 5000 bp. This Ladder is ideal for sizing larger PCR products (>1kb) and most cloning applications.
Contents: 1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Ladder Properties: • Ten discrete fragments ranging from 300 bp to 5000 bp
Fragment
Size (bp)
Mass (ng)
1
5000
88
2
4000
78
3
3000
67
4
2500
58
5
2000
50
6
1500
43
7
1000
25
8
700
34
9
500
33
10
300
24
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
