The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
Step
Component
Cat. 70100 (24 preps)
Cat. 70110, 70120, 70130, 70140 (96 preps)
Amplicon PCR (PCR 1)
MGX Master Mix
330 µL
1,320 µL
16S V1-V2 Primer Mix
70 µL
280 µL
Index PCR (PCR 2)
Indexing Master Mix
660 µL
2 x 1,320 µL
N7xx Index Primer
50 µL
50 µL
S5xx Index Primer
70 µL
70 µL
PCR Clean-Up
Resuspension Buffer
2 x 1,250 µL
2 x 5,000 µL
Nuclease-free water
1,250 µL
1 x 6,000 µL
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Product Description
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Product Name: Pigeon group A rotavirus isothermal detetction fluorescence kit
Target Species: Pigeon
Type: Fluorescence Kit
Storage Temperature: -20°C
Sample Type: Serum, Plasma, Whole Blood
Technical Parameters:
Product Name
Pigeon group A rotavirus isothermal isothermal detetction fluorescence kit
Type
Fluorescence Kit
Sample Volume
50 μl
Sample Type
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Storage Temperature
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Target Disease
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Target Species
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Applications:
Amp-future bio Livestock Disease Kit is an innovative animal health care product designed to detect and diagnose livestock diseases. Developed by a Chinese research team, this kit is a simple and reliable solution to test and treat livestock diseases. With its detection limit of 0.1 ng/ml, users can obtain accurate results quickly and accurately. The test time takes from 5-20 minutes, and the kit typically contains 48 tests/kit. Furthermore, it is easy to store with its cold storage temperature of -20°C.
The Amp-future bio Livestock Disease Kit provides an innovative solution for animal health care. It is an essential product for veterinary professionals, pet owners, and animal health researchers to detect and diagnose livestock diseases. With its simple and reliable detection and diagnosis, this kit is a must-have for anyone who wants to ensure the health of their animals.
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
Our technical support staff is available to answer any questions you may have about the product.
We offer online tutorials and user guides to help you understand the product better.
We provide technical assistance to help troubleshoot any issues you may be having with the product.
We offer a warranty on our products.
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FAQ:
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Q: How does Livestock Disease Kit work? A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
Q: How long does it take to get results from Livestock Disease Kit? A: Livestock Disease Kit can give a result within 5-20 mins.
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Q: Does Livestock Disease Kit require professional equipment? A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine
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Staphylococcus Aureus PCR Detection Kit for Isothermal Fluorescence Detector
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We use a reputable shipping company with tracking options and signature confirmation. Depending on the shipping destination, the package may require additional documentation, such as a Certificate of Origin or an invoice.
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Exceptional value for money
Rapid detection of all clinically relevant subtypes
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
