16S V3-V5 Library Preparation Kit for Illumina

Description 

The DM3260 FluoroBand™ 1 KB Plus (0.1-10 kb) Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM3260 is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easier reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank. 

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months
• Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 10,000 bp 

Concentration 

87 µg/ 500 µl  

Recommended loading volume 

5 µl/ well

Storage

Protected from light 
Room temperature for 6 months
4°C for 12 months 
-20°C for 24 months 

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Overview

• Rapid and simple procedure
• Excellent quality and yield of DNA
• Process a broad spectrum of plant species and filamentous fungi
• Isolate total DNA including pathogen DNA without phenol 
• Available in spin column format and 96-well format for high throughput applications

These kits provide a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing.

Plant/Fungi DNA Isolation Kit (Spin Column)

Complete 10 purifications in 45 minutes. This kit offers a maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

Plant/Fungi DNA Isolation 96-Well Kit (High Throughput)

For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Complete 96 purifications in 50 minutes. This kit offers a maximum loading volume of 500 μL per well, and a maximum binding capacity of 50 μg per well.

Plant/Fungi DNA Isolation Kit (Magnetic Bead System)

The DNA is bound to the surface of the magnetic beads under optimized buffer conditions and released using a low salt buffer system. The Plant DNA Isolation Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.

Plant/Fungi DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

For high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.

Details

Supporting Data

Figure 1 / 7

Previous

Next

Click for expanded view

Kit Specifications – Spin Column
Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material:Plant TissuesFungi (wet weight)	100 mg 100 mg
Average Yields* 50 mg Tomato Leaves 50 mg Grape Leaves 50 mg Peach Leaves 50 mg Plum Leaves 50 mg Pine Needles Botrytis cinerea (50 mg wet weight) Fusarium sp. (50 mg wet weight) Aspergillus niger (50 mg wet weight)	18 µg 10 µg 10 µg 10 µg 5 µg 1.5 µg 2 µg 4 µg
Time to Complete 10 Purifications	45 minutes

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
 

* Average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage. 

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature, except for the RNAse which should be stored at -20°C. This kit is stable for 1 year after the date of shipment.

Select Plants and Fungi that can be used with the Plant/Fungi DNA Purification Kits

Plants	Plants (Cont’d)	Fungi
Tomato	Turnip	Aspergillus niger
Grape	Chinese Cabbage	Mucor racemosus
Peach	Radish	Cladosporium cladosporioides
Plum	Komatsuna	Fusarium oxysporum
Pine Needles	Apricot	Penicillium sp.
Raspberry	Sweet Potato	Botrytis cinerea (Botryotinia fuckeliana)
Strawberry	Hydrangea	Pichia sp.
Legumes	Fig	Rhizopus oryzae
Prosopis cineraria (Ghaf)	Turf	Alternaria tenuissima
Sorghum grass	Cherry	Fusarium sp.
Tobacco	Saintpaulia
Arabidopsis	Lotus
Lichen	Carrot
Corn seed	Hansen
Sunflower seed	Pistachio
Olive seed
Soybean seed

Component	Cat. 26200 (50 preps)	Cat. 26250 (250 preps)	Cat. 26900 (192 preps)	Cat. 58200 (50 preps)	Cat. 62400 (192 preps)
Lysis Buffer L	30 mL	1 x 30 mL 2 x 60 mL	2 x 60 mL	60 mL	2 x 60 mL
Binding Buffer I	7 mL	1 x 7 mL 1 x 25 mL	25 mL	7 mL	25 mL
Solution WN	18 mL	1 x 18 mL 1 x 55 mL	55 mL	18 mL	55 mL
Wash Solution A	38 mL	2 x 38 mL	2 x 38 mL	–	–
Elution Buffer B	15 mL	30 mL	30 mL	8 mL	30 mL
RNAse A	1 vial (80 μL)	5 vials (5 x 80 μL)	1 vial	1 vial	1 vial
Magnetic Bead Suspension	–	–	–	4 x 1.1 mL	2 x 8.5 mL
Filter Columns	50	250	–	–	–
Spin Columns	50	250	–	–	–
Collection Tubes	100	500	–	–	–
96-Well Plate	–	–	2	–	2
96-Well Collection Plate	–	–	2	–	–
Adhesive Tape	–	–	4	–	2
Elution Tubes (1.7 mL)	50	250	–	50	–
96-Well Elution Plate	–	–	2	–	2
Product Insert	1	1	1	1	1